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Literature summary for 1.1.1.159 extracted from

  • Baron, S.F.; Franklund, C.V.; Hylemon, P.B.
    Cloning, sequencing, and expression of the gene coding for bile acid 7alpha-hydroxysteroid dehydrogenase from Eubacterium sp. strain VPI 12708 (1991), J. Bacteriol., 173, 4558-4569.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.159

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli DH5alpha, amino acid sequence analysis and comparison with other dehydrogenases Eubacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
 wild type and recombinant proteins, non-denaturing PAGE Eubacterium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
bile acids + NAD(P)+ Eubacterium sp.
-
 ?
-
 r
bile acids + NAD(P)+ Eubacterium sp. VPI 12708
-
 ?
-
 r
additional information Eubacterium sp. cholic acid inducible ?
-
 ?
additional information Eubacterium sp. VPI 12708 cholic acid inducible ?
-
 ?

Organism

Organism UniProt Comment Textmining
Eubacterium sp.
-
-
-
Eubacterium sp. VPI 12708
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and recombinant from Escherichia coli Eubacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
332
-
 NADP+, recombinant enzyme from Escherichia coli Eubacterium sp.
374
-
 NADP+ Eubacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bile acids + NAD(P)+
-
 Eubacterium sp. ?
-
 r
bile acids + NAD(P)+
-
 Eubacterium sp. VPI 12708 ?
-
 r
cholic acid + NAD(P)+ inducible by cholic acid Eubacterium sp. 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oic acid + NADH
-
 r
cholic acid + NAD(P)+ inducible by cholic acid Eubacterium sp. VPI 12708 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oic acid + NADH
-
 r
glycochenodeoxycholic acid + NAD(P)+
-
 Eubacterium sp. N-[3alpha-hydroxy-7,24-dioxocholan-24-yl]glycine + NAD(P)H
-
 r
glycochenodeoxycholic acid + NAD(P)+
-
 Eubacterium sp. VPI 12708 N-[3alpha-hydroxy-7,24-dioxocholan-24-yl]glycine + NAD(P)H
-
 r
additional information cholic acid inducible Eubacterium sp. ?
-
 ?
additional information cholic acid inducible Eubacterium sp. VPI 12708 ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 32000-33000 Eubacterium sp.

Cofactor

Cofactor Comment Organism Structure
NAD+ nearly no activity with Eubacterium sp.
NADP+
-
 Eubacterium sp.