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Literature summary for 1.1.1.159 extracted from
- Purification and properties of three kinds of alpha-hydroxysteroid dehydrogenase from Brevibacterium fuscum DC33 (1988), J. Ferment. Technol., 66, 145-152.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.22 | - |
cholic acid | - |
Brevibacterium fuscum |  |
| 0.25 | - |
NADH | with dehydrocholic acid | Brevibacterium fuscum | |
| 0.27 | - |
NAD+ | with cholic acid | Brevibacterium fuscum | |
| 0.38 | - |
12-oxolithocholic acid | - |
Brevibacterium fuscum | |
| 0.4 | - |
NAD+ | with 12-ketolithocholic acid | Brevibacterium fuscum | |
| 0.7 | - |
Dehydrocholic acid | - |
Brevibacterium fuscum | |
| 0.99 | - |
7,12-Diketolithocholic acid | - |
Brevibacterium fuscum | |
| 1.3 | - |
NADH | with 7,12-diketolithocholic acid | Brevibacterium fuscum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
4 * 27000, SDS-PAGE | Brevibacterium fuscum |
| 104000 | - |
gel filtration | Brevibacterium fuscum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brevibacterium fuscum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Brevibacterium fuscum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 400 | - |
7,12-diketolithocholic acid, NADH | Brevibacterium fuscum |
| 1600 | - |
cholic acid, NAD+ | Brevibacterium fuscum |
| 1700 | - |
12-ketolithocholic acid, NAD+ | Brevibacterium fuscum |
| 2900 | - |
dehydrocholic acid, NADH | Brevibacterium fuscum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 12-ketochenodeoxycholic acid + NAD+ | - |
Brevibacterium fuscum | 7,12-diketochenodeoxycholic acid + NADH | - |
r | |
| 12-oxolithocholic acid + NAD+ | - |
Brevibacterium fuscum | ? | - |
r | |
| 7,12-diketolithocholic acid + NADH | - |
Brevibacterium fuscum | 12-ketochenodeoxycholic acid + NAD+ | - |
r | |
| chenodeoxycholic acid + NAD(P)+ | i.e. 3alpha,7alpha-dihydroxy-5beta-cholan-24-oic acid | Brevibacterium fuscum | 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NAD(P)H | - |
? | |
| cholic acid + NAD(P)+ | i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate | Brevibacterium fuscum | 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oic acid + NADH | - |
r | |
| dehydrocholic acid + NADH | - |
Brevibacterium fuscum | 3,12-diketolithocholic acid + NAD+ | - |
r | |
| glycochenodeoxycholic acid + NAD(P)+ | - |
Brevibacterium fuscum | N-[3alpha-hydroxy-7,24-dioxocholan-24-yl]glycine + NAD(P)H | - |
? | |
| glycocholic acid + NAD+ | i.e. N-[3alpha,7alpha,12alpha-trihydroxy-24-oxocholan-24-yl]glycine | Brevibacterium fuscum | N-[3alpha,12alpha-dihydroxy-7,24-dioxo-5beta-cholan-24-yl]glycine + NADH | - |
? | |
| taurocholic acid + NAD+ | i.e. 2[[3alpha,7alpha,12alpha-trihydroxy-24-oxo-5beta-cholan-24-yl]amino]ethane sulfonic acid | Brevibacterium fuscum | 2[[3alpha,12alpha-dihydroxy-7,24-dioxo-5beta-cholan-24-yl]amino]ethane sulfonic acid + NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 27000, SDS-PAGE | Brevibacterium fuscum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 30 | - |
Brevibacterium fuscum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 45 | - |
Brevibacterium fuscum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
stable below | Brevibacterium fuscum |
| 40 | - |
inactivation at | Brevibacterium fuscum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
value for reduction of dehydrocholic acid below 5.2, not measurable due to insolubility of substrate | Brevibacterium fuscum |
| 5.3 | - |
dehydrocholic acid reduction | Brevibacterium fuscum |
| 10.5 | - |
oxidation of cholic acid | Brevibacterium fuscum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.7 | 10.5 | 5°C overnight | Brevibacterium fuscum |
| 6 | 11 | - |
Brevibacterium fuscum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Brevibacterium fuscum | |
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