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show all sequences of 1.1.1.156

Purification and properties of two different dihydroxyacetone reductases in Gluconobacter suboxydans grown on glycerol

Adachi, O.; Ano, Y.; Shinagawa, E.; Matsushita, K.; Biosci. Biotechnol. Biochem. 72, 2124-2132 (2008)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0083
-
dihydroxyacetone
at pH 6.5, in 50 mM potassium phosphate buffer, at 25°C
Gluconobacter oxydans
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Gluconobacter oxydans
5737
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
SDS-PAGE
Gluconobacter oxydans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Gluconobacter oxydans
-
strain IFO 3255
-
Gluconobacter oxydans IFO
-
strain IFO 3255
-
Purification (Commentary)
Commentary
Organism
DEAE-cellulose column chromatography, hydroxyapatite column chromatography, BD Sepharose column chromatography, and DEAE-Sephadex A-50 gel filtration
Gluconobacter oxydans
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.06
-
crude extract, at 25°C
Gluconobacter oxydans
13.71
-
after 228.5fold purification, at 25°C
Gluconobacter oxydans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dihydroxyacetone + NADH + H+
less than 10% of the enzyme activity of NADPH-DHAR with NADPH is observed with NADH
696816
Gluconobacter oxydans
glycerol + NAD+
-
-
-
r
dihydroxyacetone + NADH + H+
less than 10% of the enzyme activity of NADPH-DHAR with NADPH is observed with NADH
696816
Gluconobacter oxydans IFO
glycerol + NAD+
-
-
-
r
dihydroxyacetone + NADPH + H+
-
696816
Gluconobacter oxydans
glycerol + NADP+
-
-
-
r
dihydroxyacetone + NADPH + H+
-
696816
Gluconobacter oxydans IFO
glycerol + NADP+
-
-
-
r
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
dependent on
Gluconobacter oxydans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
dependent on
Gluconobacter oxydans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0083
-
dihydroxyacetone
at pH 6.5, in 50 mM potassium phosphate buffer, at 25°C
Gluconobacter oxydans
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Gluconobacter oxydans
5737
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
SDS-PAGE
Gluconobacter oxydans
Purification (Commentary) (protein specific)
Commentary
Organism
DEAE-cellulose column chromatography, hydroxyapatite column chromatography, BD Sepharose column chromatography, and DEAE-Sephadex A-50 gel filtration
Gluconobacter oxydans
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.06
-
crude extract, at 25°C
Gluconobacter oxydans
13.71
-
after 228.5fold purification, at 25°C
Gluconobacter oxydans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dihydroxyacetone + NADH + H+
less than 10% of the enzyme activity of NADPH-DHAR with NADPH is observed with NADH
696816
Gluconobacter oxydans
glycerol + NAD+
-
-
-
r
dihydroxyacetone + NADH + H+
less than 10% of the enzyme activity of NADPH-DHAR with NADPH is observed with NADH
696816
Gluconobacter oxydans IFO
glycerol + NAD+
-
-
-
r
dihydroxyacetone + NADPH + H+
-
696816
Gluconobacter oxydans
glycerol + NADP+
-
-
-
r
dihydroxyacetone + NADPH + H+
-
696816
Gluconobacter oxydans IFO
glycerol + NADP+
-
-
-
r
Other publictions for EC 1.1.1.156
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723568
Chen
Comparative analysis on the ke ...
Dunaliella salina
PLoS ONE
7
e37578
2012
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1
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2
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1
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-
1
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2
-
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-
-
1
1
-
-
-
696816
Adachi
Purification and properties of ...
Gluconobacter oxydans, Gluconobacter oxydans IFO
Biosci. Biotechnol. Biochem.
72
2124-2132
2008
-
-
-
-
-
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-
1
1
-
1
-
-
4
-
-
1
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-
-
2
-
4
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-
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1
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1
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1
1
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1
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1
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2
-
4
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673558
Liepins
Enzymes for the NADPH-dependen ...
Trichoderma reesei
FEBS J.
273
4229-4235
2006
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-
1
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4
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1
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2
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1
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1
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1
1
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4
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1
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1
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286040
Marshall
Purification and properties of ...
Schizosaccharomyces pombe
J. Gen. Microbiol.
135
697-701
1989
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5
4
-
-
3
1
-
4
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-
1
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-
2
1
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7
1
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2
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1
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-
1
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5
-
4
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3
1
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1
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2
1
-
7
1
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2
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286041
Vasiliadis
-
Glycerol and dihydroxyacetone ...
Schizosaccharomyces octosporus, Schizosaccharomyces pombe, Schizosaccharomyces slooffiae
Arch. Microbiol.
147
263-267
1987
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3
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3
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6
2
-
3
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-
3
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3
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3
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6
2
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3
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-
286045
Van Laere
Purification and properties of ...
Phycomyces blakesleeanus
FEMS Microbiol. Lett.
30
377-381
1985
-
-
-
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3
4
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2
1
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1
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1
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1
1
-
5
1
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-
2
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1
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1
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3
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4
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2
1
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1
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1
1
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5
1
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-
2
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-
-
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-
-
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-
587013
Kong
-
Glycerol oxidation and triose ...
Schizosaccharomyces pombe
J. Gen. Microbiol.
131
1571-1579
1985
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-
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2
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1
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1
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2
1
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2
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2
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1
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1
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2
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1
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2
1
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2
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-
2
-
-
-
-
-
-
-
-
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-
81028
Gimmler
-
The intracellular distribution ...
Dunaliella parva
Z. Naturforsch. C
37
1107-1114
1982
-
-
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1
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1
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2
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1
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1
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1
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1
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2
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1
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286042
Amotz-Ben
NADP specific dihydroxyacetone ...
Dunaliella parva
FEBS Lett.
29
153-155
1973
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4
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1
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2
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2
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11
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2
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1
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1
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4
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1
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2
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11
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2
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