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show all sequences of 1.1.1.156

Purification and properties of an NADPH-dependent dihydroxyacetone reductase from Phycomyces spores

Van Laere, A.; FEMS Microbiol. Lett. 30, 377-381 (1985)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
KCl
inhibition above 100 mM
Phycomyces blakesleeanus
Mg2+
40% inhibition at 5 mM
Phycomyces blakesleeanus
Mn2+
25% inhibition at 5 mM
Phycomyces blakesleeanus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
NADPH
-
Phycomyces blakesleeanus
0.167
-
NADP+
-
Phycomyces blakesleeanus
3.9
-
dihydroxyacetone
-
Phycomyces blakesleeanus
2437
-
glycerol
-
Phycomyces blakesleeanus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
39000
gel filtration, SDS-PAGE
Phycomyces blakesleeanus
39000
-
1 * 39000, SDS-PAGE
Phycomyces blakesleeanus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glycerol + NADP+
Phycomyces blakesleeanus
involved in synthesis of glycerol during early spore germination
dihydroxyacetone + NADPH
-
Phycomyces blakesleeanus
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Phycomyces blakesleeanus
-
-
-
Purification (Commentary)
Commentary
Organism
to homogeneity, chromatography techniques
Phycomyces blakesleeanus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
sporangiospore
-
Phycomyces blakesleeanus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
31.1
-
-
Phycomyces blakesleeanus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dihydroxyacetonephosphate + NADPH
very low activity
286045
Phycomyces blakesleeanus
glycerol 3-phosphate + NADP+
-
286045
Phycomyces blakesleeanus
?
glycerol + NADP+
involved in synthesis of glycerol during early spore germination
286045
Phycomyces blakesleeanus
dihydroxyacetone + NADPH
-
286045
Phycomyces blakesleeanus
r
L-arabitol + NADP+
11% of the activity compared to glycerol
286045
Phycomyces blakesleeanus
? + NADPH
-
286045
Phycomyces blakesleeanus
?
L-glyceraldehyde + NADPH
very low activity
286045
Phycomyces blakesleeanus
L-glycerol + NADP+
-
286045
Phycomyces blakesleeanus
?
meso-erythritol + NADP+
35% of the activity compared to glycerol
286045
Phycomyces blakesleeanus
? + NADPH
-
286045
Phycomyces blakesleeanus
r
Subunits
Subunits
Commentary
Organism
monomer
1 * 39000, SDS-PAGE
Phycomyces blakesleeanus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
6.5
dihydroxyacetone reduction
Phycomyces blakesleeanus
9.4
-
glycerol oxidation
Phycomyces blakesleeanus
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
can not be replaced by NAD+
Phycomyces blakesleeanus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
can not be replaced by NAD+
Phycomyces blakesleeanus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
KCl
inhibition above 100 mM
Phycomyces blakesleeanus
Mg2+
40% inhibition at 5 mM
Phycomyces blakesleeanus
Mn2+
25% inhibition at 5 mM
Phycomyces blakesleeanus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
NADPH
-
Phycomyces blakesleeanus
0.167
-
NADP+
-
Phycomyces blakesleeanus
3.9
-
dihydroxyacetone
-
Phycomyces blakesleeanus
2437
-
glycerol
-
Phycomyces blakesleeanus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
39000
gel filtration, SDS-PAGE
Phycomyces blakesleeanus
39000
-
1 * 39000, SDS-PAGE
Phycomyces blakesleeanus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glycerol + NADP+
Phycomyces blakesleeanus
involved in synthesis of glycerol during early spore germination
dihydroxyacetone + NADPH
-
Phycomyces blakesleeanus
r
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity, chromatography techniques
Phycomyces blakesleeanus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
sporangiospore
-
Phycomyces blakesleeanus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
31.1
-
-
Phycomyces blakesleeanus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dihydroxyacetonephosphate + NADPH
very low activity
286045
Phycomyces blakesleeanus
glycerol 3-phosphate + NADP+
-
286045
Phycomyces blakesleeanus
?
glycerol + NADP+
involved in synthesis of glycerol during early spore germination
286045
Phycomyces blakesleeanus
dihydroxyacetone + NADPH
-
286045
Phycomyces blakesleeanus
r
L-arabitol + NADP+
11% of the activity compared to glycerol
286045
Phycomyces blakesleeanus
? + NADPH
-
286045
Phycomyces blakesleeanus
?
L-glyceraldehyde + NADPH
very low activity
286045
Phycomyces blakesleeanus
L-glycerol + NADP+
-
286045
Phycomyces blakesleeanus
?
meso-erythritol + NADP+
35% of the activity compared to glycerol
286045
Phycomyces blakesleeanus
? + NADPH
-
286045
Phycomyces blakesleeanus
r
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 39000, SDS-PAGE
Phycomyces blakesleeanus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
6.5
dihydroxyacetone reduction
Phycomyces blakesleeanus
9.4
-
glycerol oxidation
Phycomyces blakesleeanus
Other publictions for EC 1.1.1.156
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723568
Chen
Comparative analysis on the ke ...
Dunaliella salina
PLoS ONE
7
e37578
2012
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-
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-
1
-
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-
2
-
-
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-
1
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-
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2
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
1
1
-
-
-
696816
Adachi
Purification and properties of ...
Gluconobacter oxydans, Gluconobacter oxydans IFO
Biosci. Biotechnol. Biochem.
72
2124-2132
2008
-
-
-
-
-
-
-
1
1
-
1
-
-
4
-
-
1
-
-
-
2
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
1
-
-
-
-
1
-
-
2
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673558
Liepins
Enzymes for the NADPH-dependen ...
Trichoderma reesei
FEBS J.
273
4229-4235
2006
-
-
1
-
-
-
-
4
-
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286040
Marshall
Purification and properties of ...
Schizosaccharomyces pombe
J. Gen. Microbiol.
135
697-701
1989
-
-
-
-
-
-
5
4
-
-
3
1
-
4
-
-
1
-
-
2
1
-
7
1
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
5
-
4
-
-
3
1
-
-
-
1
-
2
1
-
7
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
286041
Vasiliadis
-
Glycerol and dihydroxyacetone ...
Schizosaccharomyces octosporus, Schizosaccharomyces pombe, Schizosaccharomyces slooffiae
Arch. Microbiol.
147
263-267
1987
-
-
-
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
6
2
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
6
2
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286045
Van Laere
Purification and properties of ...
Phycomyces blakesleeanus
FEMS Microbiol. Lett.
30
377-381
1985
-
-
-
-
-
-
3
4
-
-
2
1
-
1
-
-
1
-
-
1
1
-
5
1
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
3
-
4
-
-
2
1
-
-
-
1
-
1
1
-
5
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
587013
Kong
-
Glycerol oxidation and triose ...
Schizosaccharomyces pombe
J. Gen. Microbiol.
131
1571-1579
1985
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
1
-
-
2
1
-
2
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
2
1
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
81028
Gimmler
-
The intracellular distribution ...
Dunaliella parva
Z. Naturforsch. C
37
1107-1114
1982
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286042
Amotz-Ben
NADP specific dihydroxyacetone ...
Dunaliella parva
FEBS Lett.
29
153-155
1973
-
-
-
-
-
-
-
4
-
-
-
1
-
2
-
-
-
-
-
2
-
-
11
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
4
-
-
-
1
-
-
-
-
-
2
-
-
11
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-