BRENDA - Enzyme Database show
show all sequences of 1.1.1.156

NADP specific dihydroxyacetone reductase from Dunaliella parva

Amotz-Ben, A.; Avron, M.; FEBS Lett. 29, 153-155 (1973)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.004
-
NADPH
-
Dunaliella parva
0.015
-
NADP+
-
Dunaliella parva
0.8
-
dihydroxyacetone
-
Dunaliella parva
1400
-
glycerol
-
Dunaliella parva
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glycerol + NADP+
Dunaliella parva
low affinity for glycerol and high affinity for dihydroxyacetone promotes synthesis and accumulation of glycerol as the major osmotic regulator of the cell
dihydroxyacetone + NADPH
-
Dunaliella parva
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Dunaliella parva
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture condition:dihydroxyacetone-grown cell
-
Dunaliella parva
-
culture condition:glycerol-grown cell
-
Dunaliella parva
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2-ethanediol + NADP+
0.3% of the activity compared to glycerol
286042
Dunaliella parva
hydroxyacetaldehyde + NADPH
-
286042
Dunaliella parva
r
1,2-propanediol + NADP+
10% of the activity compared to glycerol
286042
Dunaliella parva
hydroxyacetone + NADPH
-
286042
Dunaliella parva
r
2,3-butanediol + NADP+
12% of the activity compared to glycerol
286042
Dunaliella parva
3-hydroxybutan-2-one + NADPH
-
286042
Dunaliella parva
r
dihydroxyacetonephosphate + NADPH
very low activity
286042
Dunaliella parva
glycerol 3-phosphate + NADP+
-
286042
Dunaliella parva
?
glycerol + NADP+
low affinity for glycerol and high affinity for dihydroxyacetone promotes synthesis and accumulation of glycerol as the major osmotic regulator of the cell
286042
Dunaliella parva
dihydroxyacetone + NADPH
-
286042
Dunaliella parva
r
iso-erythritol + NADP+
0.9% of the activity compared to glycerol
286042
Dunaliella parva
? + NADPH
-
286042
Dunaliella parva
r
L-glyceraldehyde + NADPH
very low activity
286042
Dunaliella parva
L-glycerol + NADP+
-
286042
Dunaliella parva
?
L-ribulose + NADPH
very low activity
286042
Dunaliella parva
? + NADP+
-
286042
Dunaliella parva
?
meso-erythritol + NADP+
0.4% of the activity compared to glycerol
286042
Dunaliella parva
? + NADPH
-
286042
Dunaliella parva
r
methylglyoxal + NADPH
very low activity
286042
Dunaliella parva
2-hydroxypropanal + NADP+
-
286042
Dunaliella parva
?
sedoheptulose + NADPH
very low activity
286042
Dunaliella parva
? + NADP+
-
286042
Dunaliella parva
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
dihydroxyacetone reduction
Dunaliella parva
9
-
glycerol oxidation
Dunaliella parva
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
can not be replaced by NAD+
Dunaliella parva
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
can not be replaced by NAD+
Dunaliella parva
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.004
-
NADPH
-
Dunaliella parva
0.015
-
NADP+
-
Dunaliella parva
0.8
-
dihydroxyacetone
-
Dunaliella parva
1400
-
glycerol
-
Dunaliella parva
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glycerol + NADP+
Dunaliella parva
low affinity for glycerol and high affinity for dihydroxyacetone promotes synthesis and accumulation of glycerol as the major osmotic regulator of the cell
dihydroxyacetone + NADPH
-
Dunaliella parva
r
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture condition:dihydroxyacetone-grown cell
-
Dunaliella parva
-
culture condition:glycerol-grown cell
-
Dunaliella parva
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2-ethanediol + NADP+
0.3% of the activity compared to glycerol
286042
Dunaliella parva
hydroxyacetaldehyde + NADPH
-
286042
Dunaliella parva
r
1,2-propanediol + NADP+
10% of the activity compared to glycerol
286042
Dunaliella parva
hydroxyacetone + NADPH
-
286042
Dunaliella parva
r
2,3-butanediol + NADP+
12% of the activity compared to glycerol
286042
Dunaliella parva
3-hydroxybutan-2-one + NADPH
-
286042
Dunaliella parva
r
dihydroxyacetonephosphate + NADPH
very low activity
286042
Dunaliella parva
glycerol 3-phosphate + NADP+
-
286042
Dunaliella parva
?
glycerol + NADP+
low affinity for glycerol and high affinity for dihydroxyacetone promotes synthesis and accumulation of glycerol as the major osmotic regulator of the cell
286042
Dunaliella parva
dihydroxyacetone + NADPH
-
286042
Dunaliella parva
r
iso-erythritol + NADP+
0.9% of the activity compared to glycerol
286042
Dunaliella parva
? + NADPH
-
286042
Dunaliella parva
r
L-glyceraldehyde + NADPH
very low activity
286042
Dunaliella parva
L-glycerol + NADP+
-
286042
Dunaliella parva
?
L-ribulose + NADPH
very low activity
286042
Dunaliella parva
? + NADP+
-
286042
Dunaliella parva
?
meso-erythritol + NADP+
0.4% of the activity compared to glycerol
286042
Dunaliella parva
? + NADPH
-
286042
Dunaliella parva
r
methylglyoxal + NADPH
very low activity
286042
Dunaliella parva
2-hydroxypropanal + NADP+
-
286042
Dunaliella parva
?
sedoheptulose + NADPH
very low activity
286042
Dunaliella parva
? + NADP+
-
286042
Dunaliella parva
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
dihydroxyacetone reduction
Dunaliella parva
9
-
glycerol oxidation
Dunaliella parva
Other publictions for EC 1.1.1.156
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723568
Chen
Comparative analysis on the ke ...
Dunaliella salina
PLoS ONE
7
e37578
2012
-
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
1
1
-
-
-
696816
Adachi
Purification and properties of ...
Gluconobacter oxydans, Gluconobacter oxydans IFO
Biosci. Biotechnol. Biochem.
72
2124-2132
2008
-
-
-
-
-
-
-
1
1
-
1
-
-
4
-
-
1
-
-
-
2
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
1
-
-
-
-
1
-
-
2
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673558
Liepins
Enzymes for the NADPH-dependen ...
Trichoderma reesei
FEBS J.
273
4229-4235
2006
-
-
1
-
-
-
-
4
-
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286040
Marshall
Purification and properties of ...
Schizosaccharomyces pombe
J. Gen. Microbiol.
135
697-701
1989
-
-
-
-
-
-
5
4
-
-
3
1
-
4
-
-
1
-
-
2
1
-
7
1
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
5
-
4
-
-
3
1
-
-
-
1
-
2
1
-
7
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
286041
Vasiliadis
-
Glycerol and dihydroxyacetone ...
Schizosaccharomyces octosporus, Schizosaccharomyces pombe, Schizosaccharomyces slooffiae
Arch. Microbiol.
147
263-267
1987
-
-
-
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
6
2
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
6
2
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286045
Van Laere
Purification and properties of ...
Phycomyces blakesleeanus
FEMS Microbiol. Lett.
30
377-381
1985
-
-
-
-
-
-
3
4
-
-
2
1
-
1
-
-
1
-
-
1
1
-
5
1
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
3
-
4
-
-
2
1
-
-
-
1
-
1
1
-
5
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
587013
Kong
-
Glycerol oxidation and triose ...
Schizosaccharomyces pombe
J. Gen. Microbiol.
131
1571-1579
1985
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
1
-
-
2
1
-
2
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
2
1
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
81028
Gimmler
-
The intracellular distribution ...
Dunaliella parva
Z. Naturforsch. C
37
1107-1114
1982
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286042
Amotz-Ben
NADP specific dihydroxyacetone ...
Dunaliella parva
FEBS Lett.
29
153-155
1973
-
-
-
-
-
-
-
4
-
-
-
1
-
2
-
-
-
-
-
2
-
-
11
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
4
-
-
-
1
-
-
-
-
-
2
-
-
11
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-