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show all sequences of 1.1.1.156

Purification and properties of glycerol:NADP+ 2-oxidoreductase from Schizisaccharomyces pombe

Marshall, J.H.; Kong, Y.C.; Sloan, J.; May, J.W.; J. Gen. Microbiol. 135, 697-701 (1989)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
80% inhibition at 1 mM
Schizosaccharomyces pombe
8-hydroxyquinoline
complete inhibition at 10 mM
Schizosaccharomyces pombe
dithiothreitol
25% inhibition at 10 mM
Schizosaccharomyces pombe
L-cysteine
40% inhibition at 10 mM
Schizosaccharomyces pombe
p-chloromercuribenzoate
strong inhibition
Schizosaccharomyces pombe
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
NADP+
-
Schizosaccharomyces pombe
0.34
-
NADPH
-
Schizosaccharomyces pombe
0.67
-
dihydroxyacetone
-
Schizosaccharomyces pombe
51
-
glycerol
-
Schizosaccharomyces pombe
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
-
1 * 30000 + 1 * 25000, SDS-PAGE
Schizosaccharomyces pombe
30000
-
1 * 30000 + 1 * 25000, SDS-PAGE
Schizosaccharomyces pombe
57000
-
gel filtration
Schizosaccharomyces pombe
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glycerol + NADP+
Schizosaccharomyces pombe
-
dihydroxyacetone + NADPH
-
Schizosaccharomyces pombe
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Schizosaccharomyces pombe
-
-
-
Purification (Commentary)
Commentary
Organism
to homogeneity, 1step chromatography
Schizosaccharomyces pombe
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture condition:dihydroxyacetone-grown cell
-
Schizosaccharomyces pombe
-
culture condition:glycerol-grown cell
-
Schizosaccharomyces pombe
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
15.7
-
-
Schizosaccharomyces pombe
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2,3-butanetriol + NADP+
18% of the activity compared to glycerol
286040
Schizosaccharomyces pombe
1,3-dihydroxybutan-2-one + NADPH
-
286040
Schizosaccharomyces pombe
?
1,2-butanediol + NADP+
19% of the activity compared to glycerol
286040
Schizosaccharomyces pombe
1-hydroxybutan-2-one + NADPH
-
286040
Schizosaccharomyces pombe
?
1,2-propanediol + NADP+
30% of the activity compared to glycerol, low reverse reaction rate
286040
Schizosaccharomyces pombe
hydroxyacetone + NADPH
-
286040
Schizosaccharomyces pombe
r
2,3-butanediol + NADP+
25% of the activity compared to glycerol, low reverse reaction rate
286040
Schizosaccharomyces pombe
3-hydroxybutan-2-one + NADPH
-
286040
Schizosaccharomyces pombe
r
glycerol + NADP+
-
286040
Schizosaccharomyces pombe
dihydroxyacetone + NADPH
-
286040
Schizosaccharomyces pombe
r
propan-2-ol + NADP+
very low activity
286040
Schizosaccharomyces pombe
acetone + NADPH
-
286040
Schizosaccharomyces pombe
?
pyruvaldehyde + NADPH
very low activity
286040
Schizosaccharomyces pombe
lactaldehyde + NADP+
-
286040
Schizosaccharomyces pombe
?
Subunits
Subunits
Commentary
Organism
dimer
1 * 30000 + 1 * 25000, SDS-PAGE
Schizosaccharomyces pombe
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
dihydroxyacetone reduction
Schizosaccharomyces pombe
9.5
-
glycerol oxidation
Schizosaccharomyces pombe
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
can not be replaced by NAD+
Schizosaccharomyces pombe
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
can not be replaced by NAD+
Schizosaccharomyces pombe
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
80% inhibition at 1 mM
Schizosaccharomyces pombe
8-hydroxyquinoline
complete inhibition at 10 mM
Schizosaccharomyces pombe
dithiothreitol
25% inhibition at 10 mM
Schizosaccharomyces pombe
L-cysteine
40% inhibition at 10 mM
Schizosaccharomyces pombe
p-chloromercuribenzoate
strong inhibition
Schizosaccharomyces pombe
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
NADP+
-
Schizosaccharomyces pombe
0.34
-
NADPH
-
Schizosaccharomyces pombe
0.67
-
dihydroxyacetone
-
Schizosaccharomyces pombe
51
-
glycerol
-
Schizosaccharomyces pombe
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
-
1 * 30000 + 1 * 25000, SDS-PAGE
Schizosaccharomyces pombe
30000
-
1 * 30000 + 1 * 25000, SDS-PAGE
Schizosaccharomyces pombe
57000
-
gel filtration
Schizosaccharomyces pombe
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glycerol + NADP+
Schizosaccharomyces pombe
-
dihydroxyacetone + NADPH
-
Schizosaccharomyces pombe
r
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity, 1step chromatography
Schizosaccharomyces pombe
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture condition:dihydroxyacetone-grown cell
-
Schizosaccharomyces pombe
-
culture condition:glycerol-grown cell
-
Schizosaccharomyces pombe
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
15.7
-
-
Schizosaccharomyces pombe
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2,3-butanetriol + NADP+
18% of the activity compared to glycerol
286040
Schizosaccharomyces pombe
1,3-dihydroxybutan-2-one + NADPH
-
286040
Schizosaccharomyces pombe
?
1,2-butanediol + NADP+
19% of the activity compared to glycerol
286040
Schizosaccharomyces pombe
1-hydroxybutan-2-one + NADPH
-
286040
Schizosaccharomyces pombe
?
1,2-propanediol + NADP+
30% of the activity compared to glycerol, low reverse reaction rate
286040
Schizosaccharomyces pombe
hydroxyacetone + NADPH
-
286040
Schizosaccharomyces pombe
r
2,3-butanediol + NADP+
25% of the activity compared to glycerol, low reverse reaction rate
286040
Schizosaccharomyces pombe
3-hydroxybutan-2-one + NADPH
-
286040
Schizosaccharomyces pombe
r
glycerol + NADP+
-
286040
Schizosaccharomyces pombe
dihydroxyacetone + NADPH
-
286040
Schizosaccharomyces pombe
r
propan-2-ol + NADP+
very low activity
286040
Schizosaccharomyces pombe
acetone + NADPH
-
286040
Schizosaccharomyces pombe
?
pyruvaldehyde + NADPH
very low activity
286040
Schizosaccharomyces pombe
lactaldehyde + NADP+
-
286040
Schizosaccharomyces pombe
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
1 * 30000 + 1 * 25000, SDS-PAGE
Schizosaccharomyces pombe
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
dihydroxyacetone reduction
Schizosaccharomyces pombe
9.5
-
glycerol oxidation
Schizosaccharomyces pombe
Other publictions for EC 1.1.1.156
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723568
Chen
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Dunaliella salina
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7
e37578
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-
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1
-
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-
2
-
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1
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2
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1
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-
1
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2
-
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-
1
1
-
-
-
696816
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Purification and properties of ...
Gluconobacter oxydans, Gluconobacter oxydans IFO
Biosci. Biotechnol. Biochem.
72
2124-2132
2008
-
-
-
-
-
-
-
1
1
-
1
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-
4
-
-
1
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-
2
-
4
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-
-
-
-
-
-
1
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-
1
-
-
-
-
-
-
1
1
-
1
-
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-
1
-
-
2
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673558
Liepins
Enzymes for the NADPH-dependen ...
Trichoderma reesei
FEBS J.
273
4229-4235
2006
-
-
1
-
-
-
-
4
-
-
1
-
-
2
-
-
-
-
-
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-
-
1
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-
-
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-
1
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1
1
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4
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-
1
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-
-
1
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-
-
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-
-
-
-
-
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-
-
-
286040
Marshall
Purification and properties of ...
Schizosaccharomyces pombe
J. Gen. Microbiol.
135
697-701
1989
-
-
-
-
-
-
5
4
-
-
3
1
-
4
-
-
1
-
-
2
1
-
7
1
-
-
-
-
2
-
-
1
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-
1
-
-
-
-
5
-
4
-
-
3
1
-
-
-
1
-
2
1
-
7
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
286041
Vasiliadis
-
Glycerol and dihydroxyacetone ...
Schizosaccharomyces octosporus, Schizosaccharomyces pombe, Schizosaccharomyces slooffiae
Arch. Microbiol.
147
263-267
1987
-
-
-
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
6
2
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
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-
3
-
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-
6
2
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286045
Van Laere
Purification and properties of ...
Phycomyces blakesleeanus
FEMS Microbiol. Lett.
30
377-381
1985
-
-
-
-
-
-
3
4
-
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2
1
-
1
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-
1
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1
1
-
5
1
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-
2
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-
1
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-
1
-
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-
3
-
4
-
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2
1
-
-
-
1
-
1
1
-
5
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
587013
Kong
-
Glycerol oxidation and triose ...
Schizosaccharomyces pombe
J. Gen. Microbiol.
131
1571-1579
1985
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
1
-
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2
1
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2
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2
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-
1
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1
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2
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1
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2
1
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2
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2
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81028
Gimmler
-
The intracellular distribution ...
Dunaliella parva
Z. Naturforsch. C
37
1107-1114
1982
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1
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1
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2
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1
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1
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1
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1
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2
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1
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286042
Amotz-Ben
NADP specific dihydroxyacetone ...
Dunaliella parva
FEBS Lett.
29
153-155
1973
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4
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1
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2
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2
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11
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2
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1
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1
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4
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1
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2
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11
-
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2
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