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show all sequences of 1.1.1.154

S-ureidoglycolate dehydrogenase: Purification and properties

Van der Drift, C.; van Helvoort, P.E.M.; Vogels, G.D.; Arch. Biochem. Biophys. 145, 465-469 (1971)

Data extracted from this reference:

General Stability
General Stability
Organism
incubation with 30% glycerol at 4°C for 3 h stabilizes activity
Arthrobacter allantoicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
glycolate
18% inhibition at 17 mM
Arthrobacter allantoicus
glyoxylate
non-competitive inhibition, Ki: 83 mM
Arthrobacter allantoicus
L-lactate
10% inhibition at 17 mM
Arthrobacter allantoicus
Zn2+
40% inhibition at 0.5 mM
Arthrobacter allantoicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
NADP+
-
Arthrobacter allantoicus
2.16
-
NAD+
-
Arthrobacter allantoicus
9.1
-
(R,S)-ureidoglycolate
-
Arthrobacter allantoicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-ureidoglycolate + NAD(P)+
Arthrobacter allantoicus
strong substrate specificity, involved in the degradation of allantoin to glyoxylate in microbes and lower animals
oxalureate + NAD(P)H
-
Arthrobacter allantoicus
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arthrobacter allantoicus
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Arthrobacter allantoicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Arthrobacter allantoicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
10.6
-
-
Arthrobacter allantoicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-ureidoglycolate + NAD(P)+
strong substrate specificity, involved in the degradation of allantoin to glyoxylate in microbes and lower animals
286034
Arthrobacter allantoicus
oxalureate + NAD(P)H
-
286034
Arthrobacter allantoicus
ir
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
-
unstable above
Arthrobacter allantoicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
8.4
sensitive to ionic strength
Arthrobacter allantoicus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
-
decrease of activity below
Arthrobacter allantoicus
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Arthrobacter allantoicus
NADP+
-
Arthrobacter allantoicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Arthrobacter allantoicus
NADP+
-
Arthrobacter allantoicus
General Stability (protein specific)
General Stability
Organism
incubation with 30% glycerol at 4°C for 3 h stabilizes activity
Arthrobacter allantoicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
glycolate
18% inhibition at 17 mM
Arthrobacter allantoicus
glyoxylate
non-competitive inhibition, Ki: 83 mM
Arthrobacter allantoicus
L-lactate
10% inhibition at 17 mM
Arthrobacter allantoicus
Zn2+
40% inhibition at 0.5 mM
Arthrobacter allantoicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
NADP+
-
Arthrobacter allantoicus
2.16
-
NAD+
-
Arthrobacter allantoicus
9.1
-
(R,S)-ureidoglycolate
-
Arthrobacter allantoicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-ureidoglycolate + NAD(P)+
Arthrobacter allantoicus
strong substrate specificity, involved in the degradation of allantoin to glyoxylate in microbes and lower animals
oxalureate + NAD(P)H
-
Arthrobacter allantoicus
ir
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Arthrobacter allantoicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Arthrobacter allantoicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
10.6
-
-
Arthrobacter allantoicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-ureidoglycolate + NAD(P)+
strong substrate specificity, involved in the degradation of allantoin to glyoxylate in microbes and lower animals
286034
Arthrobacter allantoicus
oxalureate + NAD(P)H
-
286034
Arthrobacter allantoicus
ir
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
-
unstable above
Arthrobacter allantoicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
8.4
sensitive to ionic strength
Arthrobacter allantoicus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
-
decrease of activity below
Arthrobacter allantoicus
Other publictions for EC 1.1.1.154
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
726324
Rothe
Novel insights into E. colis h ...
Escherichia coli
PLoS ONE
8
e56906
2013
-
-
1
1
11
-
-
20
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
1
-
-
20
1
-
-
1
-
-
-
-
-
1
1
1
11
-
-
-
-
20
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
-
-
20
1
-
-
-
-
-
-
-
19
19
286034
Van der Drift
S-ureidoglycolate dehydrogenas ...
Arthrobacter allantoicus
Arch. Biochem. Biophys.
145
465-469
1971
-
-
-
-
-
1
4
3
-
-
-
1
-
1
-
-
1
-
-
1
1
-
1
-
-
-
1
-
1
-
1
2
-
-
-
-
-
-
2
-
-
1
-
4
-
3
-
-
-
1
-
-
-
1
-
1
1
-
1
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-