Application | Comment | Organism |
---|---|---|
medicine | siRNA-mediated knock-down of sepiapterin reductase expression significantly reduces endogenous ornithine decarboxylase enzyme activity in neuroblastoma cells. In a cohort of 88 human neuroblastoma tumors, high SPR mRNA expression correlates significantly with poor survival prognosis | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
silico protein-protein docking simulations to define the individual amino acid residues involved in the interaction between native ornithine decarboxylase and sepiapterin reductase | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | siRNA-mediated knockdown of SPR expression, significantly reduces endogenous ornithine decarboxylase enzyme activity in neuroblastoma cells and leads to a significant and consistent decrease in cellular proliferation | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | native ornithine decarboxylase, EC 4.1.1.17, and sepiapterin reductase physically interact, in silico protein-protein docking simulations | ? | - |
? | |
sepiapterin + NADPH + H+ | Homo sapiens | - |
tetrahydrobiopterin + NADP+ | no stereochemic specification in the publication | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P35270 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293 cell | - |
Homo sapiens | - |
MYCN2 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | native ornithine decarboxylase, EC 4.1.1.17, and sepiapterin reductase physically interact, in silico protein-protein docking simulations | Homo sapiens | ? | - |
? | |
sepiapterin + NADPH + H+ | - |
Homo sapiens | tetrahydrobiopterin + NADP+ | no stereochemic specification in the publication | ? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Homo sapiens |
More | in silico protein-protein docking simulationsof enzyme SPR with ornithine decraboxylase, ODC, structure analysis, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
sepiapterin reductase | - |
Homo sapiens |
SPR | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | the knockdown of SPR leads to a significant and consistent decrease in cellular proliferation of neuroblastoma cells | Homo sapiens |
physiological function | native ornithine decarboxylase and sepiapterin reductase physically interact. The resulting heterocomplex is a compact structure, featuring two energetically and structurally equivalent binding modes both in monomer and dimer conformations. siRNA-mediated knock-down of sepiapterin reductase expression significantly reduces endogenous ornithine decarboxylase enzyme activity in neuroblastoma cells | Homo sapiens |
physiological function | sepiapterin reductase (SPR) catalyzes the last step in the biosynthesis of tetrahydrobiopterin (BH4) by converting 6-pyruvoyl tetrahydropterin to tetrahydrobiopterin. BH4 is an essential cofactor of nitric oxide synthase (NOS), which converts arginine to nitric oxide (NO) and citrulline in the urea cycle. Native ornithine decarboxylase, ODC, EC 4.1.1.17, and sepiapterin reductase physically interact, SPR is a regulator of ODC activity. SPR is implicated in neurological diseases and in cancer. SPR contributes to neuroblastoma cell proliferation and regulates ODC enzyme activity in neuroblastoma cells, ODC-SPR interaction controls polyamine-driven cellular proliferation | Homo sapiens |