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show all sequences of 1.1.1.153

Sepiapterin Reductase Mediates Chemical Redox Cycling in Lung Epithelial Cells

Yang, S.; Jan, Y.H.; Gray, J.P.; Mishin, V.; Heck, D.E.; Laskin, D.L.; Laskin, J.D.; J. Biol. Chem. 288, 19221-19237 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli
Homo sapiens
Engineering
Amino acid exchange
Commentary
Organism
D257H
mutant shows completely inhibited sepiapterin reduction. Mutation has only minimal effects on redox cycling
Homo sapiens
DELTA257-261
deletion of the C-terminal 5 amino acids almost completely eliminates enzyme activity. For redox cycling, the catalytic efficacy decreases to less than 1% of the wild type enzyme
Homo sapiens
G14S
mutations in Gly14 and Gly18 in the NADPH binding motif of sepiapterin reductase results in almost complete loss of the ability to reduce sepiapterin, and a 65-75% decrease in redox cycling. For both of these mutations, the catalytic efficiencies for redox cycling decreases to 0.2% of wild type sepiapterin reductase
Homo sapiens
G18D
mutations in Gly14 and Gly18 in the NADPH binding motif of sepiapterin reductase results in almost complete loss of the ability to reduce sepiapterin, and a 65-75% decrease in redox cycling. For both of these mutations, the catalytic efficiencies for redox cycling decreases to 0.2% of wild type sepiapterin reductase
Homo sapiens
K174L
catalytic efficiencies (Kcat/Km) for sepiapterin reduction of S157A mutant and K174L mutant decreases to 1.8% and 0.8% of wild type sepiapterin reductase, respectively, and for redox cycling to 6.8% and 1.4%, respectively
Homo sapiens
M205G
mutation leads to marked reductions in the activities of both sepiapterin reduction and redox cycling. The catalytic efficiency of N99A and M205G for sepiapterin reduction decreases to approximately 1% and 5%, respectively, and for redox cycling, 5% and 25%, respectively, when compared to the wild type enzyme
Homo sapiens
N99A
mutation leads to marked reductions in the activities of both sepiapterin reduction and redox cycling. The catalytic efficiency of N99A and M205G for sepiapterin reduction decreases to approximately 1% and 5%, respectively, and for redox cycling, 5% and 25%, respectively, when compared to the wild type enzyme
Homo sapiens
R42G
mutation leads to a 90% reduction in sepiapterin reduction activity and a 50% reduction in redox cycling activity. The catalytic efficiencies for this mutant decreases to 2% and 7% of wild type sepiapterin reductase for sepiapterin reduction and redox cycling, respectively
Homo sapiens
S157A
catalytic efficiencies (Kcat/Km) for sepiapterin reduction of S157A mutant and K174L mutant decreases to 1.8% and 0.8% of wild type sepiapterin reductase, respectively, and for redox cycling to 6.8% and 1.4%, respectively
Homo sapiens
Y259A
mutation of Tyr259, a unique potential phosphorylation site in the C-terminal substrate transfer motif, has no major effects on sepiapterin reduction and redox cycling activity
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,2-naphthoquinone
-
Homo sapiens
9,10-phenanthrenequinone
-
Homo sapiens
benzoquinone
-
Homo sapiens
dicoumarol
-
Homo sapiens
indomethacin
-
Homo sapiens
N-acetylserotonin
-
Homo sapiens
phenylquinone
-
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0019
-
9,10-phenanthrenequinone
mutant D257H, pH 6.4, 37C
Homo sapiens
0.0021
-
1,2-naphthoquinone
wild-type, pH 6.4, 37C
Homo sapiens
0.0032
-
9,10-phenanthrenequinone
mutant S157A, pH 6.4, 37C
Homo sapiens
0.0088
-
9,10-phenanthrenequinone
wild-type, pH 6.4, 37C
Homo sapiens
0.0133
-
9,10-phenanthrenequinone
mutant M205G, pH 6.4, 37C
Homo sapiens
0.0196
-
9,10-phenanthrenequinone
mutant Y259A, pH 6.4, 37C
Homo sapiens
0.025
-
sepiapterin
mutant Y259A, pH 6.4, 37C; wild-type, pH 6.4, 37C
Homo sapiens
0.027
-
1,4-Naphthoquinone
wild-type, pH 6.4, 37C
Homo sapiens
0.027
-
9,10-phenanthrenequinone
mutant K174L, pH 6.4, 37C
Homo sapiens
0.045
-
sepiapterin
mutant R42G, pH 6.4, 37C
Homo sapiens
0.0501
-
9,10-phenanthrenequinone
mutant G14S, pH 6.4, 37C; mutant R42G, pH 6.4, 37C
Homo sapiens
0.0503
-
sepiapterin
mutant S157A, pH 6.4, 37C
Homo sapiens
0.0606
-
sepiapterin
mutant D257H, pH 6.4, 37C
Homo sapiens
0.086
-
2,3-dimethoxynaphthalene-1,4-dione
wild-type, pH 6.4, 37C
Homo sapiens
0.0872
-
menadione
wild-type, pH 6.4, 37C
Homo sapiens
0.094
-
sepiapterin
mutant N99A, pH 6.4, 37C
Homo sapiens
0.116
-
9,10-phenanthrenequinone
mutant DELTA257-261, pH 6.4, 37C
Homo sapiens
0.126
-
9,10-phenanthrenequinone
mutant N99A, pH 6.4, 37C
Homo sapiens
0.133
-
sepiapterin
mutant M205G, pH 6.4, 37C
Homo sapiens
0.169
-
sepiapterin
mutant K174L, pH 6.4, 37C
Homo sapiens
0.464
-
9,10-phenanthrenequinone
mutant G18D, pH 6.4, 37C
Homo sapiens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
SDS-PAGE
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
lung epithelial cell
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.8
-
pH 6.7, 37C
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2-naphthoquinone + NADPH
-
725527
Homo sapiens
?
-
-
-
?
1,4-naphthoquinone + NADPH
-
725527
Homo sapiens
?
-
-
-
?
2,3-dimethoxynaphthalene-1,4-dione + NADPH
-
725527
Homo sapiens
?
-
-
-
?
9,10-phenanthrenequinone + NADPH
-
725527
Homo sapiens
?
-
-
-
?
menadione + NADPH
-
725527
Homo sapiens
?
-
-
-
?
sepiapterin + NADPH
-
725527
Homo sapiens
7,8-dihydrobiopterin + NADP+
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Homo sapiens
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0048
-
9,10-phenanthrenequinone
mutant S157A, pH 6.4, 37C
Homo sapiens
0.0053
-
sepiapterin
mutant N99A, pH 6.4, 37C
Homo sapiens
0.0056
-
9,10-phenanthrenequinone
mutant G14S, pH 6.4, 37C
Homo sapiens
0.0058
-
9,10-phenanthrenequinone
mutant DELTA257-261, pH 6.4, 37C
Homo sapiens
0.0058
-
sepiapterin
mutant S157A, pH 6.4, 37C
Homo sapiens
0.027
-
9,10-phenanthrenequinone
mutant K174L, pH 6.4, 37C
Homo sapiens
0.048
-
9,10-phenanthrenequinone
mutant G18D, pH 6.4, 37C
Homo sapiens
0.053
-
sepiapterin
mutant R42G, pH 6.4, 37C
Homo sapiens
0.09
-
sepiapterin
mutant K174L, pH 6.4, 37C
Homo sapiens
0.098
-
9,10-phenanthrenequinone
mutant R42G, pH 6.4, 37C
Homo sapiens
0.107
-
9,10-phenanthrenequinone
mutant D257H, pH 6.4, 37C
Homo sapiens
0.146
-
9,10-phenanthrenequinone
wild-type, pH 6.4, 37C
Homo sapiens
0.156
-
sepiapterin
mutant D257H, pH 6.4, 37C
Homo sapiens
0.22
-
9,10-phenanthrenequinone
mutant M205G, pH 6.4, 37C
Homo sapiens
0.29
-
9,10-phenanthrenequinone
mutant N99A, pH 6.4, 37C
Homo sapiens
0.428
-
sepiapterin
mutant M205G, pH 6.4, 37C
Homo sapiens
0.45
-
1,4-Naphthoquinone
wild-type, pH 6.4, 37C
Homo sapiens
0.6
-
sepiapterin
mutant Y259A, pH 6.4, 37C
Homo sapiens
0.627
-
1,2-naphthoquinone
wild-type, pH 6.4, 37C
Homo sapiens
0.627
-
9,10-phenanthrenequinone
wild-type, pH 6.4, 37C
Homo sapiens
0.805
-
9,10-phenanthrenequinone
mutant Y259A, pH 6.4, 37C
Homo sapiens
1.43
-
2,3-dimethoxynaphthalene-1,4-dione
wild-type, pH 6.4, 37C
Homo sapiens
1.45
-
menadione
wild-type, pH 6.4, 37C
Homo sapiens
1.61
-
sepiapterin
wild-type, pH 6.4, 37C
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.4
-
assay at
Homo sapiens
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0005
-
phenylquinone
wild-type, pH 6.7, 37C
Homo sapiens
0.0009
-
benzoquinone
wild-type, pH 6.7, 37C
Homo sapiens
0.0009
-
N-acetylserotonin
wild-type, pH 6.7, 37C
Homo sapiens
0.0027
-
indomethacin
wild-type, pH 6.7, 37C
Homo sapiens
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.0002
-
wild-type, pH 6.7, 37C
Homo sapiens
dicoumarol
0.0016
-
wild-type, pH 6.7, 37C
Homo sapiens
phenylquinone
0.0026
-
wild-type, pH 6.7, 37C
Homo sapiens
N-acetylserotonin
0.0028
-
wild-type, pH 6.7, 37C
Homo sapiens
benzoquinone
0.0036
-
wild-type, pH 6.7, 37C
Homo sapiens
9,10-phenanthrenequinone
0.0081
-
wild-type, pH 6.7, 37C
Homo sapiens
indomethacin
0.0089
-
wild-type, pH 6.7, 37C
Homo sapiens
1,2-naphthoquinone
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli
Homo sapiens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D257H
mutant shows completely inhibited sepiapterin reduction. Mutation has only minimal effects on redox cycling
Homo sapiens
DELTA257-261
deletion of the C-terminal 5 amino acids almost completely eliminates enzyme activity. For redox cycling, the catalytic efficacy decreases to less than 1% of the wild type enzyme
Homo sapiens
G14S
mutations in Gly14 and Gly18 in the NADPH binding motif of sepiapterin reductase results in almost complete loss of the ability to reduce sepiapterin, and a 65-75% decrease in redox cycling. For both of these mutations, the catalytic efficiencies for redox cycling decreases to 0.2% of wild type sepiapterin reductase
Homo sapiens
G18D
mutations in Gly14 and Gly18 in the NADPH binding motif of sepiapterin reductase results in almost complete loss of the ability to reduce sepiapterin, and a 65-75% decrease in redox cycling. For both of these mutations, the catalytic efficiencies for redox cycling decreases to 0.2% of wild type sepiapterin reductase
Homo sapiens
K174L
catalytic efficiencies (Kcat/Km) for sepiapterin reduction of S157A mutant and K174L mutant decreases to 1.8% and 0.8% of wild type sepiapterin reductase, respectively, and for redox cycling to 6.8% and 1.4%, respectively
Homo sapiens
M205G
mutation leads to marked reductions in the activities of both sepiapterin reduction and redox cycling. The catalytic efficiency of N99A and M205G for sepiapterin reduction decreases to approximately 1% and 5%, respectively, and for redox cycling, 5% and 25%, respectively, when compared to the wild type enzyme
Homo sapiens
N99A
mutation leads to marked reductions in the activities of both sepiapterin reduction and redox cycling. The catalytic efficiency of N99A and M205G for sepiapterin reduction decreases to approximately 1% and 5%, respectively, and for redox cycling, 5% and 25%, respectively, when compared to the wild type enzyme
Homo sapiens
R42G
mutation leads to a 90% reduction in sepiapterin reduction activity and a 50% reduction in redox cycling activity. The catalytic efficiencies for this mutant decreases to 2% and 7% of wild type sepiapterin reductase for sepiapterin reduction and redox cycling, respectively
Homo sapiens
S157A
catalytic efficiencies (Kcat/Km) for sepiapterin reduction of S157A mutant and K174L mutant decreases to 1.8% and 0.8% of wild type sepiapterin reductase, respectively, and for redox cycling to 6.8% and 1.4%, respectively
Homo sapiens
Y259A
mutation of Tyr259, a unique potential phosphorylation site in the C-terminal substrate transfer motif, has no major effects on sepiapterin reduction and redox cycling activity
Homo sapiens
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.0002
-
wild-type, pH 6.7, 37C
Homo sapiens
dicoumarol
0.0016
-
wild-type, pH 6.7, 37C
Homo sapiens
phenylquinone
0.0026
-
wild-type, pH 6.7, 37C
Homo sapiens
N-acetylserotonin
0.0028
-
wild-type, pH 6.7, 37C
Homo sapiens
benzoquinone
0.0036
-
wild-type, pH 6.7, 37C
Homo sapiens
9,10-phenanthrenequinone
0.0081
-
wild-type, pH 6.7, 37C
Homo sapiens
indomethacin
0.0089
-
wild-type, pH 6.7, 37C
Homo sapiens
1,2-naphthoquinone
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,2-naphthoquinone
-
Homo sapiens
9,10-phenanthrenequinone
-
Homo sapiens
benzoquinone
-
Homo sapiens
dicoumarol
-
Homo sapiens
indomethacin
-
Homo sapiens
N-acetylserotonin
-
Homo sapiens
phenylquinone
-
Homo sapiens
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0005
-
phenylquinone
wild-type, pH 6.7, 37C
Homo sapiens
0.0009
-
benzoquinone
wild-type, pH 6.7, 37C
Homo sapiens
0.0009
-
N-acetylserotonin
wild-type, pH 6.7, 37C
Homo sapiens
0.0027
-
indomethacin
wild-type, pH 6.7, 37C
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0019
-
9,10-phenanthrenequinone
mutant D257H, pH 6.4, 37C
Homo sapiens
0.0021
-
1,2-naphthoquinone
wild-type, pH 6.4, 37C
Homo sapiens
0.0032
-
9,10-phenanthrenequinone
mutant S157A, pH 6.4, 37C
Homo sapiens
0.0088
-
9,10-phenanthrenequinone
wild-type, pH 6.4, 37C
Homo sapiens
0.0133
-
9,10-phenanthrenequinone
mutant M205G, pH 6.4, 37C
Homo sapiens
0.0196
-
9,10-phenanthrenequinone
mutant Y259A, pH 6.4, 37C
Homo sapiens
0.025
-
sepiapterin
mutant Y259A, pH 6.4, 37C; wild-type, pH 6.4, 37C
Homo sapiens
0.027
-
1,4-Naphthoquinone
wild-type, pH 6.4, 37C
Homo sapiens
0.027
-
9,10-phenanthrenequinone
mutant K174L, pH 6.4, 37C
Homo sapiens
0.045
-
sepiapterin
mutant R42G, pH 6.4, 37C
Homo sapiens
0.0501
-
9,10-phenanthrenequinone
mutant G14S, pH 6.4, 37C; mutant R42G, pH 6.4, 37C
Homo sapiens
0.0503
-
sepiapterin
mutant S157A, pH 6.4, 37C
Homo sapiens
0.0606
-
sepiapterin
mutant D257H, pH 6.4, 37C
Homo sapiens
0.086
-
2,3-dimethoxynaphthalene-1,4-dione
wild-type, pH 6.4, 37C
Homo sapiens
0.0872
-
menadione
wild-type, pH 6.4, 37C
Homo sapiens
0.094
-
sepiapterin
mutant N99A, pH 6.4, 37C
Homo sapiens
0.116
-
9,10-phenanthrenequinone
mutant DELTA257-261, pH 6.4, 37C
Homo sapiens
0.126
-
9,10-phenanthrenequinone
mutant N99A, pH 6.4, 37C
Homo sapiens
0.133
-
sepiapterin
mutant M205G, pH 6.4, 37C
Homo sapiens
0.169
-
sepiapterin
mutant K174L, pH 6.4, 37C
Homo sapiens
0.464
-
9,10-phenanthrenequinone
mutant G18D, pH 6.4, 37C
Homo sapiens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
SDS-PAGE
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
lung epithelial cell
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.8
-
pH 6.7, 37C
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2-naphthoquinone + NADPH
-
725527
Homo sapiens
?
-
-
-
?
1,4-naphthoquinone + NADPH
-
725527
Homo sapiens
?
-
-
-
?
2,3-dimethoxynaphthalene-1,4-dione + NADPH
-
725527
Homo sapiens
?
-
-
-
?
9,10-phenanthrenequinone + NADPH
-
725527
Homo sapiens
?
-
-
-
?
menadione + NADPH
-
725527
Homo sapiens
?
-
-
-
?
sepiapterin + NADPH
-
725527
Homo sapiens
7,8-dihydrobiopterin + NADP+
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Homo sapiens
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0048
-
9,10-phenanthrenequinone
mutant S157A, pH 6.4, 37C
Homo sapiens
0.0053
-
sepiapterin
mutant N99A, pH 6.4, 37C
Homo sapiens
0.0056
-
9,10-phenanthrenequinone
mutant G14S, pH 6.4, 37C
Homo sapiens
0.0058
-
9,10-phenanthrenequinone
mutant DELTA257-261, pH 6.4, 37C
Homo sapiens
0.0058
-
sepiapterin
mutant S157A, pH 6.4, 37C
Homo sapiens
0.027
-
9,10-phenanthrenequinone
mutant K174L, pH 6.4, 37C
Homo sapiens
0.048
-
9,10-phenanthrenequinone
mutant G18D, pH 6.4, 37C
Homo sapiens
0.053
-
sepiapterin
mutant R42G, pH 6.4, 37C
Homo sapiens
0.09
-
sepiapterin
mutant K174L, pH 6.4, 37C
Homo sapiens
0.098
-
9,10-phenanthrenequinone
mutant R42G, pH 6.4, 37C
Homo sapiens
0.107
-
9,10-phenanthrenequinone
mutant D257H, pH 6.4, 37C
Homo sapiens
0.146
-
9,10-phenanthrenequinone
wild-type, pH 6.4, 37C
Homo sapiens
0.156
-
sepiapterin
mutant D257H, pH 6.4, 37C
Homo sapiens
0.22
-
9,10-phenanthrenequinone
mutant M205G, pH 6.4, 37C
Homo sapiens
0.29
-
9,10-phenanthrenequinone
mutant N99A, pH 6.4, 37C
Homo sapiens
0.428
-
sepiapterin
mutant M205G, pH 6.4, 37C
Homo sapiens
0.45
-
1,4-Naphthoquinone
wild-type, pH 6.4, 37C
Homo sapiens
0.6
-
sepiapterin
mutant Y259A, pH 6.4, 37C
Homo sapiens
0.627
-
1,2-naphthoquinone
wild-type, pH 6.4, 37C
Homo sapiens
0.627
-
9,10-phenanthrenequinone
wild-type, pH 6.4, 37C
Homo sapiens
0.805
-
9,10-phenanthrenequinone
mutant Y259A, pH 6.4, 37C
Homo sapiens
1.43
-
2,3-dimethoxynaphthalene-1,4-dione
wild-type, pH 6.4, 37C
Homo sapiens
1.45
-
menadione
wild-type, pH 6.4, 37C
Homo sapiens
1.61
-
sepiapterin
wild-type, pH 6.4, 37C
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.4
-
assay at
Homo sapiens
Other publictions for EC 1.1.1.153
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725527
Yang
Sepiapterin Reductase Mediates ...
Homo sapiens
J. Biol. Chem.
288
19221-19237
2013
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7
21
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24
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10
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7
4
21
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1
1
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6
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1
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24
1
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-
695487
Gao
Sepiapterin reductase regulati ...
Bos taurus
Am. J. Physiol. Heart Circ. Physiol.
297
H331-H339
2009
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1
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698671
Hirakawa
Expression analysis of the ald ...
Homo sapiens, Mus musculus
J. Biochem.
146
51-60
2009
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2
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5
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4
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698883
Meng
The silkworm mutant lemon (lem ...
Bombyx mori
J. Biol. Chem.
284
11698-11705
2009
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1
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2
1
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1
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4
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1
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1
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2
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1
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1
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1
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1
1
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-
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-
700603
McHugh
Polymorphisms of sepiapterin r ...
Homo sapiens
Pharmacogenet. Genomics
19
330-337
2009
-
1
1
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-
-
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1
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1
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684899
Takazawa
A brain-specific decrease of t ...
Mus musculus
Biochem. Biophys. Res. Commun.
367
787-792
2008
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1
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2
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4
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689027
Sawabe
Cellular uptake of sepiapterin ...
Homo sapiens
Mol. Genet. Metab.
94
410-416
2008
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1
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3
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1
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4
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695294
Supangat
Role of Phe-99 and Trp-196 of ...
Chlorobaculum tepidum
Acta Biochim. Biophys. Sin. (Shanghai)
40
513-518
2008
-
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1
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2
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3
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3
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1
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2
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1
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685875
Tobin
Sepiapterin reductase expressi ...
Homo sapiens
Brain Res.
1139
42-47
2007
-
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1
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1
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3
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2
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2
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2
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4
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686117
Widder
Regulation of tetrahydrobiopte ...
Homo sapiens
Circ. Res.
101
830-838
2007
1
-
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1
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3
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1
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3
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686747
Kim
Tetrahydropteridine deficiency ...
Dictyostelium discoideum
FEBS Lett.
581
5430-5434
2007
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1
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3
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3
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667828
Choi
Functional role of sepiapterin ...
Dictyostelium discoideum, Dictyostelium discoideum AX2
Biochim. Biophys. Acta
1760
877-882
2006
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7
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11
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1
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11
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7
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11
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1
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668883
Thoeny
Mutations in the BH4-metaboliz ...
Homo sapiens
Hum. Mutat.
27
870-878
2006
-
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1
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6
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1
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2
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1
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669482
Supangat
Structure of Chlorobium tepidu ...
Chlorobaculum tepidum
J. Biol. Chem.
281
2249-2256
2006
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1
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5
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1
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655640
Choi
Sepiapterin reductases from Ch ...
Chlorobaculum tepidum, Chlorobium limicola
FEMS Microbiol. Lett.
242
95-99
2005
-
-
2
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2
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6
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2
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6
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2
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6
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667067
Supangat
Expression, purification, crys ...
Chlorobaculum tepidum
Acta Crystallogr. Sect. F
61
202-204
2005
-
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1
1
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1
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2
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1
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668611
Choi
D-threo-tetrahydrobiopterin is ...
Dictyostelium discoideum, Dictyostelium discoideum AX2
FEBS Lett.
579
3085-3089
2005
-
-
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1
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7
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10
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12
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2
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1
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7
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12
-
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655225
Fujimoto
Effect of antisense oligodeoxy ...
Rattus norvegicus
Chem. Biol. Interact.
143-144
583-586
2003
-
-
1
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1
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1
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1
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1
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1
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1
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2
-
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-
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-
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-
-
-
-
656958
Negishi
Localization of sepiapterin re ...
Oryzias latipes, Rattus norvegicus
Pigment Cell Res.
16
501-503
2003
-
-
-
-
-
-
-
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1
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2
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8
-
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1
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2
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2
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2
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1
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2
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1
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2
-
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-
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-
-
-
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655138
Ikemoto
Localization of sepiapterin re ...
Homo sapiens, Mus musculus
Brain Res.
954
237-246
2002
-
-
1
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-
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2
2
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3
-
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1
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13
-
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4
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2
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1
2
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2
2
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1
-
13
-
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4
-
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-
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-
-
-
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-
-
-
-
286028
Fujimoto
Role of the conserved Ser-Tyr- ...
Rattus norvegicus
Chem. Biol. Interact.
130-132
825-832
2001
-
-
1
-
4
-
-
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-
-
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1
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1
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-
-
-
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7
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1
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4
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1
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-
-
7
-
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-
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-
-
-
-
-
-
-
-
-
286030
Kim
Characterization of recombinan ...
Dictyostelium discoideum, Phycomyces sp.
Mol. Cells
10
405-410
2000
-
-
1
-
-
-
2
2
-
-
3
1
-
5
-
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-
-
-
-
1
1
1
1
-
-
-
1
-
-
-
-
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-
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1
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-
-
-
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2
-
2
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3
1
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-
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-
-
1
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286026
Cho
-
Sepiapterin reductase producin ...
Chlorobaculum tepidum
Biochem. J.
340
497-503
1999
-
-
-
-
-
-
3
3
-
-
2
1
-
1
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-
1
1
-
-
1
1
8
1
1
-
1
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
3
-
3
-
-
2
1
-
-
-
1
-
-
1
1
8
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
286032
Auerbach
The 1.25 A crystal structure o ...
Mus musculus
EMBO J.
16
7219-7230
1997
-
-
1
1
-
-
4
-
-
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1
-
2
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1
-
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-
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1
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1
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1
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4
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1
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1
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1
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
286029
Ruiz-Vazquez
Characterization of sepiapteri ...
Drosophila melanogaster, Drosophila melanogaster Oregon R
Comp. Biochem. Physiol. B
113
131-136
1996
-
-
-
-
-
-
3
1
-
-
-
2
-
7
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
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-
-
-
-
-
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-
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-
-
3
-
1
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-
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2
-
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-
-
-
1
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
286025
Maier
Purification and properties of ...
Homo sapiens
Adv. Exp. Med. Biol.
338
199-202
1993
-
-
-
-
-
-
-
2
-
-
3
1
-
2
-
-
1
-
-
2
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
3
1
-
-
-
1
-
2
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286031
Yoon
-
Isolation and characterization ...
Drosophila melanogaster, Drosophila melanogaster Oregon R
Pteridines
4
43-50
1993
-
-
-
-
-
-
6
2
-
-
2
2
-
5
-
-
1
1
-
-
-
1
12
1
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
2
-
-
2
2
-
-
-
1
-
-
-
1
12
1
1
-
1
-
-
1
-
-
-
-
-
-
-
-
286033
Dowling
Sepiapterin reductase and ALR2 ...
Bos taurus
Adv. Exp. Med. Biol.
328
313-324
1993
-
-
-
-
-
-
-
-
-
-
2
1
-
2
-
-
1
-
-
2
1
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
1
-
2
1
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286027
Smith
New inhibitors of sepiapterin ...
Bos taurus
J. Biol. Chem.
267
5599-5607
1992
-
-
-
-
-
-
11
-
1
-
-
1
-
3
-
-
1
-
-
2
-
-
1
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11
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1
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1
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1
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2
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1
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-
286010
Citron
Isolation and expression of ra ...
Rattus norvegicus
Proc. Natl. Acad. Sci. USA
87
6436-6440
1990
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-
1
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1
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3
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1
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2
1
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2
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1
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1
1
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1
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1
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2
1
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2
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-
286017
Werner
Tetrahydrobiopterin biosynthet ...
Drosophila melanogaster, Drosophila melanogaster Oregon R, Homo sapiens
J. Biol. Chem.
265
3189-3192
1990
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-
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3
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7
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1
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6
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4
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1
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1
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3
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1
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6
-
-
4
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-
286018
Kerler
Analysis of the tetrahydrobiop ...
Mus musculus
J. Cell. Physiol.
142
268-271
1990
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1
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3
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4
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1
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1
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1
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1
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4
-
1
2
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-
286016
Fere
Sepiapterin reductase in cultu ...
Homo sapiens
Biochem. Biophys. Res. Commun.
148
1475-1481
1987
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-
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1
2
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1
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2
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6
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2
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1
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1
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1
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6
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2
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286019
Katoh
Isomerization of 6-lactoyl tet ...
Rattus norvegicus
J. Biochem.
101
275-278
1987
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1
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1
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1
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1
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1
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1
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1
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1
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3
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1
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286024
Smith
On the role of sepiapterin red ...
Rattus norvegicus
Arch. Biochem. Biophys.
255
254-266
1987
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-
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2
5
-
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2
2
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3
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1
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12
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11
2
1
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1
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1
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1
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2
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5
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2
2
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1
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12
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11
2
1
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1
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286021
Sueoka
Carbonyl reductase activity of ...
Rattus norvegicus
Biochim. Biophys. Acta
843
193-198
1985
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-
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13
14
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1
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2
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1
1
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2
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16
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2
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13
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2
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13
13
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14
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1
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1
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2
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16
-
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-
286022
Curtius
Tetrahydrobiopterin biosynthes ...
Homo sapiens
Eur. J. Biochem.
148
413-419
1985
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1
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3
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1
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5
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1
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1
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1
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1
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5
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2
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286020
Katoh
Sepiapterin reductase exhibits ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
118
859-866
1984
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-
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4
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2
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1
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1
1
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3
1
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14
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2
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2
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1
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1
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4
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2
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1
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3
1
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14
-
2
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2
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286015
Sueoka
Purification and characterizat ...
Rattus norvegicus
Biochim. Biophys. Acta
717
265-271
1982
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5
2
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2
2
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2
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1
1
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2
1
1
2
1
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1
1
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1
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1
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1
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1
5
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2
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2
2
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1
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2
1
1
2
1
-
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1
1
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-
286023
Katoh
Direct inhibition of brain sep ...
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
Biochem. Biophys. Res. Commun.
105
75-81
1982
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9
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6
-
161
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1
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2
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8
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1
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1
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1
8
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1
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9
8
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6
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1
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2
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8
-
1
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1
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286014
Katoh
Sepiapterin reductase in blood ...
Canis lupus familiaris, Cavia porcellus, Gallus gallus, Mus musculus, Oryctolagus cuniculus, Rattus norvegicus, Rattus norvegicus Donryu, Rattus norvegicus Sprague-Dawley, Sus scrofa
Biochim. Biophys. Acta
370
378-388
1974
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9
-
176
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25
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9
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7
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7
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9
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25
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9
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286013
Katoh
Sepiapterin reductase from hor ...
Equus caballus, Rattus norvegicus
Arch. Biochem. Biophys.
146
202-214
1971
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2
55
9
2
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5
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3
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1
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13
1
2
19
-
2
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6
-
2
7
-
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6
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7
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2
6
55
-
9
2
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5
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1
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13
1
2
19
-
2
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-
6
-
2
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-
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-
286011
Nagai
Studies on sepiapterin reducta ...
Rattus norvegicus
Arch. Biochem. Biophys.
126
426-435
1968
-
-
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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-
667296
Nagai
-
Studies on sepiapterin reducta ...
Rattus norvegicus
Arch. Biochem. Biophys.
126
420-435
1968
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-
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1
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1
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1
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2
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1
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1
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1
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1
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1
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1
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2
-
1
-
-
-
1
-
-
-
-
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-
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-
286012
Matsubara
Sepiapterin reductase ...
Rattus norvegicus
Biochim. Biophys. Acta
122
202-212
1966
-
-
-
-
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-
2
1
-
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-
1
-
1
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1
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1
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1
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1
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1
2
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2
-
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2
-
1
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1
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1
-
1
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1
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1
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1
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-