Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D257H | mutant shows completely inhibited sepiapterin reduction. Mutation has only minimal effects on redox cycling | Homo sapiens |
DELTA257-261 | deletion of the C-terminal 5 amino acids almost completely eliminates enzyme activity. For redox cycling, the catalytic efficacy decreases to less than 1% of the wild type enzyme | Homo sapiens |
G14S | mutations in Gly14 and Gly18 in the NADPH binding motif of sepiapterin reductase results in almost complete loss of the ability to reduce sepiapterin, and a 65-75% decrease in redox cycling. For both of these mutations, the catalytic efficiencies for redox cycling decreases to 0.2% of wild type sepiapterin reductase | Homo sapiens |
G18D | mutations in Gly14 and Gly18 in the NADPH binding motif of sepiapterin reductase results in almost complete loss of the ability to reduce sepiapterin, and a 65-75% decrease in redox cycling. For both of these mutations, the catalytic efficiencies for redox cycling decreases to 0.2% of wild type sepiapterin reductase | Homo sapiens |
K174L | catalytic efficiencies (Kcat/Km) for sepiapterin reduction of S157A mutant and K174L mutant decreases to 1.8% and 0.8% of wild type sepiapterin reductase, respectively, and for redox cycling to 6.8% and 1.4%, respectively | Homo sapiens |
M205G | mutation leads to marked reductions in the activities of both sepiapterin reduction and redox cycling. The catalytic efficiency of N99A and M205G for sepiapterin reduction decreases to approximately 1% and 5%, respectively, and for redox cycling, 5% and 25%, respectively, when compared to the wild type enzyme | Homo sapiens |
N99A | mutation leads to marked reductions in the activities of both sepiapterin reduction and redox cycling. The catalytic efficiency of N99A and M205G for sepiapterin reduction decreases to approximately 1% and 5%, respectively, and for redox cycling, 5% and 25%, respectively, when compared to the wild type enzyme | Homo sapiens |
R42G | mutation leads to a 90% reduction in sepiapterin reduction activity and a 50% reduction in redox cycling activity. The catalytic efficiencies for this mutant decreases to 2% and 7% of wild type sepiapterin reductase for sepiapterin reduction and redox cycling, respectively | Homo sapiens |
S157A | catalytic efficiencies (Kcat/Km) for sepiapterin reduction of S157A mutant and K174L mutant decreases to 1.8% and 0.8% of wild type sepiapterin reductase, respectively, and for redox cycling to 6.8% and 1.4%, respectively | Homo sapiens |
Y259A | mutation of Tyr259, a unique potential phosphorylation site in the C-terminal substrate transfer motif, has no major effects on sepiapterin reduction and redox cycling activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,2-naphthoquinone | - |
Homo sapiens | |
9,10-phenanthrenequinone | - |
Homo sapiens | |
benzoquinone | - |
Homo sapiens | |
dicoumarol | - |
Homo sapiens | |
indomethacin | - |
Homo sapiens | |
N-acetylserotonin | - |
Homo sapiens | |
phenylquinone | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0019 | - |
9,10-phenanthrenequinone | mutant D257H, pH 6.4, 37°C | Homo sapiens | |
0.0021 | - |
1,2-naphthoquinone | wild-type, pH 6.4, 37°C | Homo sapiens | |
0.0032 | - |
9,10-phenanthrenequinone | mutant S157A, pH 6.4, 37°C | Homo sapiens | |
0.0088 | - |
9,10-phenanthrenequinone | wild-type, pH 6.4, 37°C | Homo sapiens | |
0.0133 | - |
9,10-phenanthrenequinone | mutant M205G, pH 6.4, 37°C | Homo sapiens | |
0.0196 | - |
9,10-phenanthrenequinone | mutant Y259A, pH 6.4, 37°C | Homo sapiens | |
0.025 | - |
sepiapterin | mutant Y259A, pH 6.4, 37°C | Homo sapiens | |
0.025 | - |
sepiapterin | wild-type, pH 6.4, 37°C | Homo sapiens | |
0.027 | - |
9,10-phenanthrenequinone | mutant K174L, pH 6.4, 37°C | Homo sapiens | |
0.027 | - |
1,4-Naphthoquinone | wild-type, pH 6.4, 37°C | Homo sapiens | |
0.045 | - |
sepiapterin | mutant R42G, pH 6.4, 37°C | Homo sapiens | |
0.0501 | - |
9,10-phenanthrenequinone | mutant G14S, pH 6.4, 37°C | Homo sapiens | |
0.0501 | - |
9,10-phenanthrenequinone | mutant R42G, pH 6.4, 37°C | Homo sapiens | |
0.0503 | - |
sepiapterin | mutant S157A, pH 6.4, 37°C | Homo sapiens | |
0.0606 | - |
sepiapterin | mutant D257H, pH 6.4, 37°C | Homo sapiens | |
0.086 | - |
2,3-dimethoxynaphthalene-1,4-dione | wild-type, pH 6.4, 37°C | Homo sapiens | |
0.0872 | - |
menadione | wild-type, pH 6.4, 37°C | Homo sapiens | |
0.094 | - |
sepiapterin | mutant N99A, pH 6.4, 37°C | Homo sapiens | |
0.116 | - |
9,10-phenanthrenequinone | mutant DELTA257-261, pH 6.4, 37°C | Homo sapiens | |
0.126 | - |
9,10-phenanthrenequinone | mutant N99A, pH 6.4, 37°C | Homo sapiens | |
0.133 | - |
sepiapterin | mutant M205G, pH 6.4, 37°C | Homo sapiens | |
0.169 | - |
sepiapterin | mutant K174L, pH 6.4, 37°C | Homo sapiens | |
0.464 | - |
9,10-phenanthrenequinone | mutant G18D, pH 6.4, 37°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lung epithelial cell | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.8 | - |
pH 6.7, 37°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-naphthoquinone + NADPH | - |
Homo sapiens | ? | - |
? | |
1,4-naphthoquinone + NADPH + H+ | - |
Homo sapiens | ? | - |
? | |
2,3-dimethoxynaphthalene-1,4-dione + NADPH | - |
Homo sapiens | ? | - |
? | |
9,10-phenanthrenequinone + NADPH | - |
Homo sapiens | ? | - |
? | |
menadione + NADPH | - |
Homo sapiens | ? | - |
? | |
sepiapterin + NADPH + H+ | - |
Homo sapiens | 7,8-dihydrobiopterin + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
sepiapterin reductase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0048 | - |
9,10-phenanthrenequinone | mutant S157A, pH 6.4, 37°C | Homo sapiens | |
0.0053 | - |
sepiapterin | mutant N99A, pH 6.4, 37°C | Homo sapiens | |
0.0056 | - |
9,10-phenanthrenequinone | mutant G14S, pH 6.4, 37°C | Homo sapiens | |
0.0058 | - |
9,10-phenanthrenequinone | mutant DELTA257-261, pH 6.4, 37°C | Homo sapiens | |
0.0058 | - |
sepiapterin | mutant S157A, pH 6.4, 37°C | Homo sapiens | |
0.027 | - |
9,10-phenanthrenequinone | mutant K174L, pH 6.4, 37°C | Homo sapiens | |
0.048 | - |
9,10-phenanthrenequinone | mutant G18D, pH 6.4, 37°C | Homo sapiens | |
0.053 | - |
sepiapterin | mutant R42G, pH 6.4, 37°C | Homo sapiens | |
0.09 | - |
sepiapterin | mutant K174L, pH 6.4, 37°C | Homo sapiens | |
0.098 | - |
9,10-phenanthrenequinone | mutant R42G, pH 6.4, 37°C | Homo sapiens | |
0.107 | - |
9,10-phenanthrenequinone | mutant D257H, pH 6.4, 37°C | Homo sapiens | |
0.146 | - |
9,10-phenanthrenequinone | wild-type, pH 6.4, 37°C | Homo sapiens | |
0.156 | - |
sepiapterin | mutant D257H, pH 6.4, 37°C | Homo sapiens | |
0.22 | - |
9,10-phenanthrenequinone | mutant M205G, pH 6.4, 37°C | Homo sapiens | |
0.29 | - |
9,10-phenanthrenequinone | mutant N99A, pH 6.4, 37°C | Homo sapiens | |
0.428 | - |
sepiapterin | mutant M205G, pH 6.4, 37°C | Homo sapiens | |
0.45 | - |
1,4-Naphthoquinone | wild-type, pH 6.4, 37°C | Homo sapiens | |
0.6 | - |
sepiapterin | mutant Y259A, pH 6.4, 37°C | Homo sapiens | |
0.627 | - |
9,10-phenanthrenequinone | wild-type, pH 6.4, 37°C | Homo sapiens | |
0.627 | - |
1,2-naphthoquinone | wild-type, pH 6.4, 37°C | Homo sapiens | |
0.805 | - |
9,10-phenanthrenequinone | mutant Y259A, pH 6.4, 37°C | Homo sapiens | |
1.43 | - |
2,3-dimethoxynaphthalene-1,4-dione | wild-type, pH 6.4, 37°C | Homo sapiens | |
1.45 | - |
menadione | wild-type, pH 6.4, 37°C | Homo sapiens | |
1.61 | - |
sepiapterin | wild-type, pH 6.4, 37°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
assay at | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
phenylquinone | wild-type, pH 6.7, 37°C | Homo sapiens | |
0.0009 | - |
N-acetylserotonin | wild-type, pH 6.7, 37°C | Homo sapiens | |
0.0009 | - |
benzoquinone | wild-type, pH 6.7, 37°C | Homo sapiens | |
0.0027 | - |
indomethacin | wild-type, pH 6.7, 37°C | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0002 | - |
wild-type, pH 6.7, 37°C | Homo sapiens | dicoumarol | |
0.0016 | - |
wild-type, pH 6.7, 37°C | Homo sapiens | phenylquinone | |
0.0026 | - |
wild-type, pH 6.7, 37°C | Homo sapiens | N-acetylserotonin | |
0.0028 | - |
wild-type, pH 6.7, 37°C | Homo sapiens | benzoquinone | |
0.0036 | - |
wild-type, pH 6.7, 37°C | Homo sapiens | 9,10-phenanthrenequinone | |
0.0081 | - |
wild-type, pH 6.7, 37°C | Homo sapiens | indomethacin | |
0.0089 | - |
wild-type, pH 6.7, 37°C | Homo sapiens | 1,2-naphthoquinone |