Cloned (Comment) | Organism |
---|---|
recombinant protein expressed in Escherichia coli | Dictyostelium discoideum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Melatonin | - |
Dictyostelium discoideum | |
N-acetylserotonin | - |
Dictyostelium discoideum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
sepiapterin | - |
Dictyostelium discoideum | |
0.052 | - |
NADPH | - |
Dictyostelium discoideum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29969 | - |
x * 29969, determined by cDNA sequence | Dictyostelium discoideum |
30000 | - |
x * 30000, Western blot analysis of native enzyme from crude cell extract | Dictyostelium discoideum |
32372 | - |
x * 32372, SDS-PAGE, includes His-tag residues | Dictyostelium discoideum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-(S)-lactoyl-7,8-dihydropterin + NADPH | Dictyostelium discoideum | - |
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyostelium discoideum | - |
- |
- |
Phycomyces sp. | - |
- |
- |
Storage Stability | Organism |
---|---|
-70°C, after dialyzation against Pipes, pH 7.5, 10% glycerol | Dictyostelium discoideum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-(S)-lactoyl-7,8-dihydropterin + NADPH | - |
Dictyostelium discoideum | 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32372, SDS-PAGE, includes His-tag residues | Dictyostelium discoideum |
? | x * 30000, Western blot analysis of native enzyme from crude cell extract | Dictyostelium discoideum |
? | x * 29969, determined by cDNA sequence | Dictyostelium discoideum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Dictyostelium discoideum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
most active, sodium phosphate buffer | Dictyostelium discoideum |