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show all sequences of 1.1.1.153

Sepiapterin reductase from horse liver: purification and properties of the enzyme

Katoh, S.; Arch. Biochem. Biophys. 146, 202-214 (1971)

Data extracted from this reference:

General Stability
General Stability
Organism
bovine serum albumin stabilizes
Equus caballus
NADP+ stabilizes
Equus caballus
Inhibitors
Inhibitors
Commentary
Organism
Structure
2,4-dinitrophenol
37% inhibition of the reductive reaction at 0.01 mM; slight
Equus caballus
2,4-dinitrophenol
-
Rattus norvegicus
6,7-Diethylpterin-H2
-
Equus caballus
6,7-dimethyldihydropterin
0.05 mM: 70% inhibition
Equus caballus
6,7-dimethyllumazine
slight
Equus caballus
6,7-dimethylpterin
0.05 mM: 46% inhibition; 46% inhibition of the reductive reaction at 0.05 mM
Equus caballus
6,7-dimethylpterin-H2
70% inhibition of the reductive reaction at 0.05 mM
Equus caballus
6-Carboxypterin
77% inhibition of the reductive reaction at 0.05 mM; IC50 0.014 mM, 0.05 mM: 77% inhibition
Equus caballus
6-Carboxypterin
-
Rattus norvegicus
6-Hydroxymethylpterin
0.05 mM: 23% inhibition; 23% inhibition of the reductive reaction at 0.05 mM
Equus caballus
6-methyldihydropterin
0.05 mM: 59% inhibition
Equus caballus
6-methylpterin
10% inhibition of the reductive reaction at 0.05 mM; slight
Equus caballus
6-Methylpterin-H2
59% inhibition of the reductive reaction at 0.05 mM
Equus caballus
7-Methylpterin
0.05 mM: 18% inhibition; 18% inhibition of the reductive reaction at 0.05 mM
Equus caballus
acetic acid
IC50 0.45 mM, pH 6.5
Equus caballus
Benzoic acid
0.03 mM: 40% inhibition; 40% inhibition of the reductive reaction at 0.03 mM
Equus caballus
biopterin
0.05 mM: 24% inhibition; 24% inhibition of the reductive reaction at 0.05 mM
Equus caballus
biopterin-H2
31% inhibition of the reductive reaction at 0.05 mM
Equus caballus
D,L-Lactic acid
0.03 mM, 20% inhibition
Equus caballus
D-erythro-neopterin
14% inhibition of the reductive reaction at 0.05 mM
Equus caballus
D-erythro-neopterin-H2
7% inhibition of the reductive reaction at 0.05 mM
Equus caballus
dihydrobiopterin
0.05 mM: 31% inhibition
Equus caballus
dihydrobiopterin
-
Rattus norvegicus
dihydroxanthopterin
slight
Equus caballus
DL-lactic acid
20% inhibition of the reductive reaction at 0.03 mM
Equus caballus
formic acid
slight
Equus caballus
iodoacetate
72% inhibition of the reductive reaction at 0.1 mM
Equus caballus
Isosepiapterin
83% inhibition of the reductive reaction at 0.05 mM; IC50 0.0065 mM, 0.05 mM: 83% inhibition
Equus caballus
Isoxanthopterin
0.05 mM: very slight
Equus caballus
KCN
25% inhibition of the reductive reaction at 2.5 mM; slight
Equus caballus
L-erythro-dihydroneopterin
0.05 mM: 29% inhibition, D-isomer: slight inhibition
Equus caballus
L-Erythro-neopterin
0.05 mM: 21% inhibition, D-isomer: slight inhibition; 21% inhibition of the reductive reaction at 0.05 mM
Equus caballus
L-Erythro-neopterin-H2
29% inhibition of the reductive reaction at 0.05 mM
Equus caballus
Leucopterin
0.05 mM: 21% inhibition; 21% inhibition of the reductive reaction at 0.05 mM
Equus caballus
monoiodoacetate
-
Equus caballus
additional information
other unconjugated pteridines, e.g. xanthopterin, that cause slight inhibition of enzymatic reduction
Equus caballus
n-butyric acid
IC50 0.032 mM, pH 6.5
Equus caballus
N-ethylmaleimide
-
Equus caballus
n-valeric acid
IC50 0.25 mM, pH 6.5
Equus caballus
NADPH
above 0.1 mM
Equus caballus
NaN3
15% inhibition of the reductive reaction at 5 mM; slight
Equus caballus
NEM
35% inhibition of the reductive reaction at 0.1 mM, 88% at 1 mM
Equus caballus
p-chloromercuribenzoate
-
Equus caballus
PCMB
93% inhibition of the reductive reaction at 0.01 mM
Equus caballus
Propionamide
0.03 mM, 14% inhibition; 14% inhibition of the reductive reaction at 0.03 mM
Equus caballus
propionic acid
43% inhibition of the reductive reaction at 0.03 mM; IC50 0.0045 mM, pH 6.5
Equus caballus
pterin
0.05 mM: 18% inhibition; 18% inhibition of the reductive reaction at 0.05 mM
Equus caballus
Pyruvic acid
9% inhibition of the reductive reaction at 0.03 mM; slight
Equus caballus
sepiapterin
above 0.05 mM
Equus caballus
sepiapterin
-
Rattus norvegicus
Sodium acetate
-
Equus caballus
Xanthopterin
slight
Equus caballus
Xanthopterin B2
slight
Equus caballus
Xanthopterin-B2
12% inhibition of the reductive reaction at 0.05 mM
Equus caballus
xanthopterin-H2
9% inhibition of the reductive reaction at 0.05 mM
Equus caballus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics
Equus caballus
0.0014
-
NADPH
pH 10.4, 25°C
Equus caballus
0.0021
-
sepiapterin
pH 10.4, 25°C
Equus caballus
0.0057
-
NADP+
pH 10.4, 25°C
Equus caballus
0.0077
-
7,8-dihydrobiopterin
pH 10.4, 25°C
Equus caballus
0.014
-
NADPH
-
Equus caballus
0.021
-
sepiapterin
-
Equus caballus
0.057
-
dihydrobiopterin
at pH 10.4
Equus caballus
0.077
-
NADP+
at pH 10.4
Equus caballus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
92% of total activity
Equus caballus
5737
-
additional information
intracellular localization, overview
Equus caballus
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6-(S)-lactoyl-7,8-dihydropterin + NADPH
Rattus norvegicus
equilibrium lies much in favor of dihydrobiopterin formation
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
Rattus norvegicus
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
Equus caballus
equilibrium lies much in favor of dihydrobiopterin formation
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
Equus caballus
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
Rattus norvegicus
reverse reaction, L-erythro and L-threo-dihydroneopterin are also oxidized, the corresponding D-isomers are inactive
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
Rattus norvegicus
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
Equus caballus
reverse reaction, L-erythro and L-threo-dihydroneopterin are also oxidized, the corresponding D-isomers are inactive
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
Equus caballus
r
sepiapterin + NADPH + H+
Equus caballus
-
7,8-dihydrobiopterin + NADP+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Equus caballus
-
-
-
Rattus norvegicus
-
; female and male Wistar rats
-
Purification (Commentary)
Commentary
Organism
liver; native enzyme 5000fold from liver by ammonium sulfate and protamine sulfate fractionation, and two times treatment by hydroxylapatite and anion exchange chromatography
Equus caballus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
blood
-
Equus caballus
-
blood
-
Rattus norvegicus
-
brain
-
Rattus norvegicus
-
erythrocyte
-
Equus caballus
-
erythrocyte
-
Rattus norvegicus
-
heart
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
liver
-
Equus caballus
-
liver
-
Rattus norvegicus
-
additional information
tissue distribution, no activity in stomach, small intestine, and muscle, overview
Rattus norvegicus
-
spleen
-
Rattus norvegicus
-
testis
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
purified enzyme
Equus caballus
Storage Stability
Storage Stability
Organism
-15°C, in 150 mM Tris-HCl buffer, pH 6.0, second hydroxylapatite fraction, for 1 week, relatively little loss of activity
Equus caballus
-15°C, purified enzyme, 0.01 M potassium phosphate buffer, pH 6.0, stable for 1 week with little loss of activity
Equus caballus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-(S)-lactoyl-7,8-dihydropterin + NADPH
in the reverse reaction, L-erythro and L-threo-dihydroneopterin are also oxidized, the corresponding L-isomers are inactive
286013
Rattus norvegicus
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
286013
Rattus norvegicus
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
in the reverse reaction, L-erythro and L-threo-dihydroneopterin are also oxidized, the corresponding L-isomers are inactive
286013
Equus caballus
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
286013
Equus caballus
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
equilibrium lies much in favor of dihydrobiopterin formation
286013
Rattus norvegicus
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
286013
Rattus norvegicus
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
equilibrium lies much in favor of dihydrobiopterin formation
286013
Equus caballus
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
286013
Equus caballus
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
reverse reaction, L-erythro and L-threo-dihydroneopterin are also oxidized, the corresponding D-isomers are inactive
286013
Rattus norvegicus
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
286013
Rattus norvegicus
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
reverse reaction, L-erythro and L-threo-dihydroneopterin are also oxidized, the corresponding D-isomers are inactive
286013
Equus caballus
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
286013
Equus caballus
r
isosepiapterin + NADPH
-
286013
Equus caballus
6-(1-hydroxypropyl)-dihydropterin + NADP+
-
286013
Equus caballus
?
isosepiapterin + NADPH
-
286013
Equus caballus
? + NADP+
-
-
-
r
L-erythro-dihydroneopterin + NADP+
D-isomer completely inactive
286013
Equus caballus
?
-
-
-
r
L-erythro-dihydroneopterin + NADP+
very slow activity, no activity with the D-isomer
286013
Equus caballus
?
-
-
-
r
L-threo-dihydroneopterin + NADP+
D-isomer completely inactive
286013
Equus caballus
?
-
-
-
r
L-threo-dihydroneopterin + NADP+
very slow activity, no activity with the D-isomer
286013
Equus caballus
?
-
-
-
r
additional information
not: 6-acetyl-7-methyldihydropterin
286013
Rattus norvegicus
?
-
-
-
-
additional information
not: 6-acetyl-7-methyldihydropterin
286013
Equus caballus
?
-
-
-
-
additional information
no activity with 6-acetyl-7-methyl-7,8-dihydropterin
286013
Equus caballus
?
-
-
-
-
sepiapterin + NADPH + H+
-
286013
Rattus norvegicus
7,8-dihydrobiopterin + NADP+
-
-
-
r
sepiapterin + NADPH + H+
-
286013
Equus caballus
7,8-dihydrobiopterin + NADP+
-
-
-
r
xanthopterin B2 + NADPH
-
286013
Equus caballus
6-(1',2'-dihydroxypropyl)-dihydrolumazine + NADP+
-
286013
Equus caballus
?
xanthopterin-B2 + NADPH
-
286013
Equus caballus
? + NADP+
-
-
-
r
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Equus caballus
25
-
assay at
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
for reduction of sepiapterin; in potassium phosphate buffer
Equus caballus
5.5
-
in potassium phosphate buffer
Rattus norvegicus
6.4
-
substrate reduction reaction, assay at
Equus caballus
6.4
-
substrate reduction reaction, assay at
Rattus norvegicus
10.4
-
substrate oxidation reaction, assay at; the reverse reaction, oxidation of 7,8-dihydrobiopterin, activity rises with rise of pH
Equus caballus
10.4
-
substrate oxidation reaction, assay at
Rattus norvegicus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5.5
-
pH 6.4 for enzyme assay; unstable
Equus caballus
5.5
-
unstable
Rattus norvegicus
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
reverse reaction, one-fifth of that with NADP+
Equus caballus
NADH
activity one-fourth of that with NADPH
Equus caballus
NADH
-
Rattus norvegicus
NADP+
-
Equus caballus
NADP+
-
Rattus norvegicus
NADPH
-
Equus caballus
NADPH
-
Rattus norvegicus
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.0045
-
IC50 0.0045 mM, pH 6.5
Equus caballus
propionic acid
0.0065
-
IC50 0.0065 mM, 0.05 mM: 83% inhibition
Equus caballus
Isosepiapterin
0.014
-
IC50 0.014 mM, 0.05 mM: 77% inhibition
Equus caballus
6-Carboxypterin
0.032
-
IC50 0.032 mM, pH 6.5
Equus caballus
n-butyric acid
0.25
-
IC50 0.25 mM, pH 6.5
Equus caballus
n-valeric acid
0.45
-
IC50 0.45 mM, pH 6.5
Equus caballus
acetic acid
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
reverse reaction, one-fifth of that with NADP+
Equus caballus
NADH
activity one-fourth of that with NADPH
Equus caballus
NADH
-
Rattus norvegicus
NADP+
-
Equus caballus
NADP+
-
Rattus norvegicus
NADPH
-
Equus caballus
NADPH
-
Rattus norvegicus
General Stability (protein specific)
General Stability
Organism
bovine serum albumin stabilizes
Equus caballus
NADP+ stabilizes
Equus caballus
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.0045
-
IC50 0.0045 mM, pH 6.5
Equus caballus
propionic acid
0.0065
-
IC50 0.0065 mM, 0.05 mM: 83% inhibition
Equus caballus
Isosepiapterin
0.014
-
IC50 0.014 mM, 0.05 mM: 77% inhibition
Equus caballus
6-Carboxypterin
0.032
-
IC50 0.032 mM, pH 6.5
Equus caballus
n-butyric acid
0.25
-
IC50 0.25 mM, pH 6.5
Equus caballus
n-valeric acid
0.45
-
IC50 0.45 mM, pH 6.5
Equus caballus
acetic acid
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2,4-dinitrophenol
37% inhibition of the reductive reaction at 0.01 mM; slight
Equus caballus
2,4-dinitrophenol
-
Rattus norvegicus
6,7-Diethylpterin-H2
-
Equus caballus
6,7-dimethyldihydropterin
0.05 mM: 70% inhibition
Equus caballus
6,7-dimethyllumazine
slight
Equus caballus
6,7-dimethylpterin
0.05 mM: 46% inhibition; 46% inhibition of the reductive reaction at 0.05 mM
Equus caballus
6,7-dimethylpterin-H2
70% inhibition of the reductive reaction at 0.05 mM
Equus caballus
6-Carboxypterin
77% inhibition of the reductive reaction at 0.05 mM; IC50 0.014 mM, 0.05 mM: 77% inhibition
Equus caballus
6-Carboxypterin
-
Rattus norvegicus
6-Hydroxymethylpterin
0.05 mM: 23% inhibition; 23% inhibition of the reductive reaction at 0.05 mM
Equus caballus
6-methyldihydropterin
0.05 mM: 59% inhibition
Equus caballus
6-methylpterin
10% inhibition of the reductive reaction at 0.05 mM; slight
Equus caballus
6-Methylpterin-H2
59% inhibition of the reductive reaction at 0.05 mM
Equus caballus
7-Methylpterin
0.05 mM: 18% inhibition; 18% inhibition of the reductive reaction at 0.05 mM
Equus caballus
acetic acid
IC50 0.45 mM, pH 6.5
Equus caballus
Benzoic acid
0.03 mM: 40% inhibition; 40% inhibition of the reductive reaction at 0.03 mM
Equus caballus
biopterin
0.05 mM: 24% inhibition; 24% inhibition of the reductive reaction at 0.05 mM
Equus caballus
biopterin-H2
31% inhibition of the reductive reaction at 0.05 mM
Equus caballus
D,L-Lactic acid
0.03 mM, 20% inhibition
Equus caballus
D-erythro-neopterin
14% inhibition of the reductive reaction at 0.05 mM
Equus caballus
D-erythro-neopterin-H2
7% inhibition of the reductive reaction at 0.05 mM
Equus caballus
dihydrobiopterin
0.05 mM: 31% inhibition
Equus caballus
dihydrobiopterin
-
Rattus norvegicus
dihydroxanthopterin
slight
Equus caballus
DL-lactic acid
20% inhibition of the reductive reaction at 0.03 mM
Equus caballus
formic acid
slight
Equus caballus
iodoacetate
72% inhibition of the reductive reaction at 0.1 mM
Equus caballus
Isosepiapterin
83% inhibition of the reductive reaction at 0.05 mM; IC50 0.0065 mM, 0.05 mM: 83% inhibition
Equus caballus
Isoxanthopterin
0.05 mM: very slight
Equus caballus
KCN
25% inhibition of the reductive reaction at 2.5 mM; slight
Equus caballus
L-erythro-dihydroneopterin
0.05 mM: 29% inhibition, D-isomer: slight inhibition
Equus caballus
L-Erythro-neopterin
0.05 mM: 21% inhibition, D-isomer: slight inhibition; 21% inhibition of the reductive reaction at 0.05 mM
Equus caballus
L-Erythro-neopterin-H2
29% inhibition of the reductive reaction at 0.05 mM
Equus caballus
Leucopterin
0.05 mM: 21% inhibition; 21% inhibition of the reductive reaction at 0.05 mM
Equus caballus
monoiodoacetate
-
Equus caballus
additional information
other unconjugated pteridines, e.g. xanthopterin, that cause slight inhibition of enzymatic reduction
Equus caballus
n-butyric acid
IC50 0.032 mM, pH 6.5
Equus caballus
N-ethylmaleimide
-
Equus caballus
n-valeric acid
IC50 0.25 mM, pH 6.5
Equus caballus
NADPH
above 0.1 mM
Equus caballus
NaN3
15% inhibition of the reductive reaction at 5 mM; slight
Equus caballus
NEM
35% inhibition of the reductive reaction at 0.1 mM, 88% at 1 mM
Equus caballus
p-chloromercuribenzoate
-
Equus caballus
PCMB
93% inhibition of the reductive reaction at 0.01 mM
Equus caballus
Propionamide
0.03 mM, 14% inhibition; 14% inhibition of the reductive reaction at 0.03 mM
Equus caballus
propionic acid
43% inhibition of the reductive reaction at 0.03 mM; IC50 0.0045 mM, pH 6.5
Equus caballus
pterin
0.05 mM: 18% inhibition; 18% inhibition of the reductive reaction at 0.05 mM
Equus caballus
Pyruvic acid
9% inhibition of the reductive reaction at 0.03 mM; slight
Equus caballus
sepiapterin
above 0.05 mM
Equus caballus
sepiapterin
-
Rattus norvegicus
Sodium acetate
-
Equus caballus
Xanthopterin
slight
Equus caballus
Xanthopterin B2
slight
Equus caballus
Xanthopterin-B2
12% inhibition of the reductive reaction at 0.05 mM
Equus caballus
xanthopterin-H2
9% inhibition of the reductive reaction at 0.05 mM
Equus caballus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics
Equus caballus
0.0014
-
NADPH
pH 10.4, 25°C
Equus caballus
0.0021
-
sepiapterin
pH 10.4, 25°C
Equus caballus
0.0057
-
NADP+
pH 10.4, 25°C
Equus caballus
0.0077
-
7,8-dihydrobiopterin
pH 10.4, 25°C
Equus caballus
0.014
-
NADPH
-
Equus caballus
0.021
-
sepiapterin
-
Equus caballus
0.057
-
dihydrobiopterin
at pH 10.4
Equus caballus
0.077
-
NADP+
at pH 10.4
Equus caballus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
92% of total activity
Equus caballus
5737
-
additional information
intracellular localization, overview
Equus caballus
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6-(S)-lactoyl-7,8-dihydropterin + NADPH
Rattus norvegicus
equilibrium lies much in favor of dihydrobiopterin formation
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
Rattus norvegicus
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
Equus caballus
equilibrium lies much in favor of dihydrobiopterin formation
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
Equus caballus
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
Rattus norvegicus
reverse reaction, L-erythro and L-threo-dihydroneopterin are also oxidized, the corresponding D-isomers are inactive
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
Rattus norvegicus
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
Equus caballus
reverse reaction, L-erythro and L-threo-dihydroneopterin are also oxidized, the corresponding D-isomers are inactive
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
Equus caballus
r
sepiapterin + NADPH + H+
Equus caballus
-
7,8-dihydrobiopterin + NADP+
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
liver; native enzyme 5000fold from liver by ammonium sulfate and protamine sulfate fractionation, and two times treatment by hydroxylapatite and anion exchange chromatography
Equus caballus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
blood
-
Equus caballus
-
blood
-
Rattus norvegicus
-
brain
-
Rattus norvegicus
-
erythrocyte
-
Equus caballus
-
erythrocyte
-
Rattus norvegicus
-
heart
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
liver
-
Equus caballus
-
liver
-
Rattus norvegicus
-
additional information
tissue distribution, no activity in stomach, small intestine, and muscle, overview
Rattus norvegicus
-
spleen
-
Rattus norvegicus
-
testis
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
purified enzyme
Equus caballus
Storage Stability (protein specific)
Storage Stability
Organism
-15°C, in 150 mM Tris-HCl buffer, pH 6.0, second hydroxylapatite fraction, for 1 week, relatively little loss of activity
Equus caballus
-15°C, purified enzyme, 0.01 M potassium phosphate buffer, pH 6.0, stable for 1 week with little loss of activity
Equus caballus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-(S)-lactoyl-7,8-dihydropterin + NADPH
in the reverse reaction, L-erythro and L-threo-dihydroneopterin are also oxidized, the corresponding L-isomers are inactive
286013
Rattus norvegicus
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
286013
Rattus norvegicus
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
in the reverse reaction, L-erythro and L-threo-dihydroneopterin are also oxidized, the corresponding L-isomers are inactive
286013
Equus caballus
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
286013
Equus caballus
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
equilibrium lies much in favor of dihydrobiopterin formation
286013
Rattus norvegicus
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
286013
Rattus norvegicus
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
equilibrium lies much in favor of dihydrobiopterin formation
286013
Equus caballus
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
286013
Equus caballus
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
reverse reaction, L-erythro and L-threo-dihydroneopterin are also oxidized, the corresponding D-isomers are inactive
286013
Rattus norvegicus
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
286013
Rattus norvegicus
r
6-(S)-lactoyl-7,8-dihydropterin + NADPH
reverse reaction, L-erythro and L-threo-dihydroneopterin are also oxidized, the corresponding D-isomers are inactive
286013
Equus caballus
6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
286013
Equus caballus
r
isosepiapterin + NADPH
-
286013
Equus caballus
6-(1-hydroxypropyl)-dihydropterin + NADP+
-
286013
Equus caballus
?
isosepiapterin + NADPH
-
286013
Equus caballus
? + NADP+
-
-
-
r
L-erythro-dihydroneopterin + NADP+
D-isomer completely inactive
286013
Equus caballus
?
-
-
-
r
L-erythro-dihydroneopterin + NADP+
very slow activity, no activity with the D-isomer
286013
Equus caballus
?
-
-
-
r
L-threo-dihydroneopterin + NADP+
D-isomer completely inactive
286013
Equus caballus
?
-
-
-
r
L-threo-dihydroneopterin + NADP+
very slow activity, no activity with the D-isomer
286013
Equus caballus
?
-
-
-
r
additional information
not: 6-acetyl-7-methyldihydropterin
286013
Rattus norvegicus
?
-
-
-
-
additional information
not: 6-acetyl-7-methyldihydropterin
286013
Equus caballus
?
-
-
-
-
additional information
no activity with 6-acetyl-7-methyl-7,8-dihydropterin
286013
Equus caballus
?
-
-
-
-
sepiapterin + NADPH + H+
-
286013
Rattus norvegicus
7,8-dihydrobiopterin + NADP+
-
-
-
r
sepiapterin + NADPH + H+
-
286013
Equus caballus
7,8-dihydrobiopterin + NADP+
-
-
-
r
xanthopterin B2 + NADPH
-
286013
Equus caballus
6-(1',2'-dihydroxypropyl)-dihydrolumazine + NADP+
-
286013
Equus caballus
?
xanthopterin-B2 + NADPH
-
286013
Equus caballus
? + NADP+
-
-
-
r
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Equus caballus
25
-
assay at
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
for reduction of sepiapterin; in potassium phosphate buffer
Equus caballus
5.5
-
in potassium phosphate buffer
Rattus norvegicus
6.4
-
substrate reduction reaction, assay at
Equus caballus
6.4
-
substrate reduction reaction, assay at
Rattus norvegicus
10.4
-
substrate oxidation reaction, assay at; the reverse reaction, oxidation of 7,8-dihydrobiopterin, activity rises with rise of pH
Equus caballus
10.4
-
substrate oxidation reaction, assay at
Rattus norvegicus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5.5
-
pH 6.4 for enzyme assay; unstable
Equus caballus
5.5
-
unstable
Rattus norvegicus
Other publictions for EC 1.1.1.153
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725527
Yang
Sepiapterin Reductase Mediates ...
Homo sapiens
J. Biol. Chem.
288
19221-19237
2013
-
-
1
-
10
-
7
21
-
-
1
-
-
3
-
-
-
-
-
1
1
-
6
-
1
-
-
24
1
-
-
-
4
-
7
-
-
1
-
-
10
-
7
7
4
21
-
-
1
-
-
-
-
-
-
1
1
-
6
-
1
-
-
24
1
-
-
-
-
-
-
-
-
-
695487
Gao
Sepiapterin reductase regulati ...
Bos taurus
Am. J. Physiol. Heart Circ. Physiol.
297
H331-H339
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698671
Hirakawa
Expression analysis of the ald ...
Homo sapiens, Mus musculus
J. Biochem.
146
51-60
2009
-
-
-
-
-
-
2
-
-
-
-
-
-
5
-
-
-
-
-
2
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
2
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698883
Meng
The silkworm mutant lemon (lem ...
Bombyx mori
J. Biol. Chem.
284
11698-11705
2009
-
-
1
-
-
-
2
1
-
-
1
-
-
4
-
-
1
-
-
1
-
-
1
-
1
-
-
-
1
1
-
1
-
-
2
-
-
1
1
-
-
-
2
2
-
1
-
-
1
-
-
-
-
1
-
1
-
-
1
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
700603
McHugh
Polymorphisms of sepiapterin r ...
Homo sapiens
Pharmacogenet. Genomics
19
330-337
2009
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684899
Takazawa
A brain-specific decrease of t ...
Mus musculus
Biochem. Biophys. Res. Commun.
367
787-792
2008
-
-
-
-
1
-
-
-
-
-
-
2
-
4
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
689027
Sawabe
Cellular uptake of sepiapterin ...
Homo sapiens
Mol. Genet. Metab.
94
410-416
2008
-
-
-
-
-
-
1
-
1
-
-
3
-
1
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
3
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695294
Supangat
Role of Phe-99 and Trp-196 of ...
Chlorobaculum tepidum
Acta Biochim. Biophys. Sin. (Shanghai)
40
513-518
2008
-
-
1
-
2
-
-
3
-
-
-
-
-
3
-
-
1
-
-
-
-
-
2
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
1
-
2
-
-
-
-
3
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
685875
Tobin
Sepiapterin reductase expressi ...
Homo sapiens
Brain Res.
1139
42-47
2007
-
-
1
-
1
-
-
-
-
-
-
3
-
2
-
-
-
-
-
2
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686117
Widder
Regulation of tetrahydrobiopte ...
Homo sapiens
Circ. Res.
101
830-838
2007
1
-
-
-
-
-
1
-
-
-
-
3
-
2
-
-
-
-
-
1
1
-
3
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
2
-
-
-
-
1
-
-
-
-
-
3
-
-
-
-
-
1
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686747
Kim
Tetrahydropteridine deficiency ...
Dictyostelium discoideum
FEBS Lett.
581
5430-5434
2007
-
-
-
-
1
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667828
Choi
Functional role of sepiapterin ...
Dictyostelium discoideum, Dictyostelium discoideum AX2
Biochim. Biophys. Acta
1760
877-882
2006
-
-
-
-
-
-
-
-
-
-
-
7
-
11
-
-
-
-
-
1
-
-
11
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
1
-
-
11
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
668883
Thoeny
Mutations in the BH4-metaboliz ...
Homo sapiens
Hum. Mutat.
27
870-878
2006
-
-
1
-
6
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
669482
Supangat
Structure of Chlorobium tepidu ...
Chlorobaculum tepidum
J. Biol. Chem.
281
2249-2256
2006
-
-
-
1
-
-
-
-
-
-
-
1
-
5
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655640
Choi
Sepiapterin reductases from Ch ...
Chlorobaculum tepidum, Chlorobium limicola
FEMS Microbiol. Lett.
242
95-99
2005
-
-
2
-
-
-
-
-
-
-
-
2
-
6
-
-
2
-
-
-
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
2
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667067
Supangat
Expression, purification, crys ...
Chlorobaculum tepidum
Acta Crystallogr. Sect. F
61
202-204
2005
-
-
1
1
-
-
-
-
-
-
-
1
-
2
-
-
1
1
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668611
Choi
D-threo-tetrahydrobiopterin is ...
Dictyostelium discoideum, Dictyostelium discoideum AX2
FEBS Lett.
579
3085-3089
2005
-
-
-
-
1
-
-
-
-
-
-
7
-
10
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655225
Fujimoto
Effect of antisense oligodeoxy ...
Rattus norvegicus
Chem. Biol. Interact.
143-144
583-586
2003
-
-
1
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656958
Negishi
Localization of sepiapterin re ...
Oryzias latipes, Rattus norvegicus
Pigment Cell Res.
16
501-503
2003
-
-
-
-
-
-
-
-
1
-
-
2
-
8
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
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1
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2
-
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-
-
-
-
655138
Ikemoto
Localization of sepiapterin re ...
Homo sapiens, Mus musculus
Brain Res.
954
237-246
2002
-
-
1
-
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2
2
-
3
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1
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13
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4
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2
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1
2
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2
2
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1
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13
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
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-
286028
Fujimoto
Role of the conserved Ser-Tyr- ...
Rattus norvegicus
Chem. Biol. Interact.
130-132
825-832
2001
-
-
1
-
4
-
-
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1
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1
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7
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1
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4
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1
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7
-
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-
-
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286030
Kim
Characterization of recombinan ...
Dictyostelium discoideum, Phycomyces sp.
Mol. Cells
10
405-410
2000
-
-
1
-
-
-
2
2
-
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3
1
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5
-
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1
1
1
1
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1
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1
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2
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2
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3
1
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-
1
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286026
Cho
-
Sepiapterin reductase producin ...
Chlorobaculum tepidum
Biochem. J.
340
497-503
1999
-
-
-
-
-
-
3
3
-
-
2
1
-
1
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-
1
1
-
-
1
1
8
1
1
-
1
-
1
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1
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1
-
-
-
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3
-
3
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2
1
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1
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-
1
1
8
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
286032
Auerbach
The 1.25 A crystal structure o ...
Mus musculus
EMBO J.
16
7219-7230
1997
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-
1
1
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4
-
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1
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2
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1
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1
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4
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1
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1
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1
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-
-
286029
Ruiz-Vazquez
Characterization of sepiapteri ...
Drosophila melanogaster, Drosophila melanogaster Oregon R
Comp. Biochem. Physiol. B
113
131-136
1996
-
-
-
-
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3
1
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2
-
7
-
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1
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2
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1
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3
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1
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1
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2
-
-
1
-
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-
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-
286025
Maier
Purification and properties of ...
Homo sapiens
Adv. Exp. Med. Biol.
338
199-202
1993
-
-
-
-
-
-
-
2
-
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3
1
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2
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1
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2
1
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1
1
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2
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3
1
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1
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2
1
-
1
1
-
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-
-
-
-
-
-
-
-
286031
Yoon
-
Isolation and characterization ...
Drosophila melanogaster, Drosophila melanogaster Oregon R
Pteridines
4
43-50
1993
-
-
-
-
-
-
6
2
-
-
2
2
-
5
-
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1
1
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1
12
1
1
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1
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1
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6
-
2
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2
2
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1
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-
1
12
1
1
-
1
-
-
1
-
-
-
-
-
-
-
-
286033
Dowling
Sepiapterin reductase and ALR2 ...
Bos taurus
Adv. Exp. Med. Biol.
328
313-324
1993
-
-
-
-
-
-
-
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2
1
-
2
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1
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2
1
1
1
1
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2
1
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1
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2
1
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286027
Smith
New inhibitors of sepiapterin ...
Bos taurus
J. Biol. Chem.
267
5599-5607
1992
-
-
-
-
-
-
11
-
1
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1
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3
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1
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2
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1
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11
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1
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1
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1
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2
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1
-
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-
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-
-
-
-
286010
Citron
Isolation and expression of ra ...
Rattus norvegicus
Proc. Natl. Acad. Sci. USA
87
6436-6440
1990
-
-
1
-
-
-
-
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1
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3
-
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1
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2
1
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2
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1
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1
1
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1
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1
-
2
1
-
2
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
286017
Werner
Tetrahydrobiopterin biosynthet ...
Drosophila melanogaster, Drosophila melanogaster Oregon R, Homo sapiens
J. Biol. Chem.
265
3189-3192
1990
-
-
-
-
-
-
-
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3
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7
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1
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6
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4
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1
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1
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3
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1
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6
-
-
4
-
-
-
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-
-
-
-
-
-
-
-
-
-
286018
Kerler
Analysis of the tetrahydrobiop ...
Mus musculus
J. Cell. Physiol.
142
268-271
1990
-
-
-
-
-
-
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1
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3
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4
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1
2
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1
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1
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1
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4
-
1
2
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
286016
Fere
Sepiapterin reductase in cultu ...
Homo sapiens
Biochem. Biophys. Res. Commun.
148
1475-1481
1987
-
-
-
-
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1
2
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1
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2
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6
-
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2
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1
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1
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1
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2
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1
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6
-
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2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286019
Katoh
Isomerization of 6-lactoyl tet ...
Rattus norvegicus
J. Biochem.
101
275-278
1987
-
-
-
-
-
-
-
-
-
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1
-
1
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1
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3
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1
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1
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1
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1
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1
-
-
3
-
-
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-
1
-
-
-
-
-
-
-
-
-
286024
Smith
On the role of sepiapterin red ...
Rattus norvegicus
Arch. Biochem. Biophys.
255
254-266
1987
-
-
-
-
-
-
2
5
-
-
2
2
-
3
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1
-
-
12
-
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11
2
1
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1
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1
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-
1
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2
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5
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2
2
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1
-
12
-
-
11
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286021
Sueoka
Carbonyl reductase activity of ...
Rattus norvegicus
Biochim. Biophys. Acta
843
193-198
1985
-
-
-
-
-
-
13
14
-
-
-
1
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2
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1
1
-
2
-
-
16
-
-
-
-
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-
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-
2
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13
-
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-
2
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-
13
13
-
14
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1
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-
1
-
2
-
-
16
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286022
Curtius
Tetrahydrobiopterin biosynthes ...
Homo sapiens
Eur. J. Biochem.
148
413-419
1985
-
-
-
-
-
-
-
-
-
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1
-
3
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1
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5
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2
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1
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1
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1
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1
-
5
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286020
Katoh
Sepiapterin reductase exhibits ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
118
859-866
1984
-
-
-
-
-
-
-
4
-
-
-
2
-
1
-
-
1
1
-
3
1
-
14
-
2
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
4
-
-
-
2
-
-
-
1
-
3
1
-
14
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
286015
Sueoka
Purification and characterizat ...
Rattus norvegicus
Biochim. Biophys. Acta
717
265-271
1982
-
-
-
-
-
-
5
2
-
-
2
2
-
2
-
-
1
1
-
2
1
1
2
1
-
-
-
-
1
1
-
1
-
-
1
-
-
-
1
-
-
-
1
5
-
2
-
-
2
2
-
-
-
1
-
2
1
1
2
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
286023
Katoh
Direct inhibition of brain sep ...
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
Biochem. Biophys. Res. Commun.
105
75-81
1982
-
-
-
-
-
-
9
-
-
-
-
6
-
161
-
-
1
-
-
2
-
-
8
-
1
-
-
-
1
-
-
1
8
-
-
-
-
-
1
-
-
-
-
9
8
-
-
-
-
6
-
-
-
1
-
2
-
-
8
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286014
Katoh
Sepiapterin reductase in blood ...
Canis lupus familiaris, Cavia porcellus, Gallus gallus, Mus musculus, Oryctolagus cuniculus, Rattus norvegicus, Rattus norvegicus Donryu, Rattus norvegicus Sprague-Dawley, Sus scrofa
Biochim. Biophys. Acta
370
378-388
1974
-
-
-
-
-
-
-
-
-
-
-
9
-
176
-
-
-
-
-
25
-
-
9
-
-
-
-
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-
-
-
7
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
25
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286013
Katoh
Sepiapterin reductase from hor ...
Equus caballus, Rattus norvegicus
Arch. Biochem. Biophys.
146
202-214
1971
-
-
-
-
-
2
55
9
2
-
-
5
-
3
-
-
1
-
-
13
1
2
19
-
2
-
-
-
6
-
2
7
-
-
6
-
-
-
7
-
-
2
6
55
-
9
2
-
-
5
-
-
-
1
-
13
1
2
19
-
2
-
-
-
6
-
2
-
-
-
-
-
-
-
286011
Nagai
Studies on sepiapterin reducta ...
Rattus norvegicus
Arch. Biochem. Biophys.
126
426-435
1968
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667296
Nagai
-
Studies on sepiapterin reducta ...
Rattus norvegicus
Arch. Biochem. Biophys.
126
420-435
1968
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286012
Matsubara
Sepiapterin reductase ...
Rattus norvegicus
Biochim. Biophys. Acta
122
202-212
1966
-
-
-
-
-
-
2
1
-
-
-
1
-
1
-
-
1
-
-
1
-
-
1
-
-
-
-
-
1
-
1
2
-
-
-
-
-
-
2
-
-
-
-
2
-
1
-
-
-
1
-
-
-
1
-
1
-
-
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-