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Literature summary for 1.1.1.153 extracted from

  • Katoh, S.
    Sepiapterin reductase from horse liver: purification and properties of the enzyme (1971), Arch. Biochem. Biophys., 146, 202-214.
    View publication on PubMed
Search for more literature for 1.1.1.153

General Stability

General Stability Organism
bovine serum albumin stabilizes Equus caballus
NADP+ stabilizes Equus caballus

Inhibitors

Inhibitors Comment Organism Structure
2,4-dinitrophenol 37% inhibition of the reductive reaction at 0.01 mM; slight Equus caballus
2,4-dinitrophenol
-
 Rattus norvegicus
6,7-Diethylpterin-H2
-
 Equus caballus
6,7-dimethyldihydropterin 0.05 mM: 70% inhibition Equus caballus
6,7-dimethyllumazine slight Equus caballus
6,7-dimethylpterin 0.05 mM: 46% inhibition; 46% inhibition of the reductive reaction at 0.05 mM Equus caballus
6,7-dimethylpterin-H2 70% inhibition of the reductive reaction at 0.05 mM Equus caballus
6-Carboxypterin 77% inhibition of the reductive reaction at 0.05 mM; IC50 0.014 mM, 0.05 mM: 77% inhibition Equus caballus
6-Carboxypterin
-
 Rattus norvegicus
6-Hydroxymethylpterin 0.05 mM: 23% inhibition; 23% inhibition of the reductive reaction at 0.05 mM Equus caballus
6-methyldihydropterin 0.05 mM: 59% inhibition Equus caballus
6-methylpterin 10% inhibition of the reductive reaction at 0.05 mM; slight Equus caballus
6-Methylpterin-H2 59% inhibition of the reductive reaction at 0.05 mM Equus caballus
7-Methylpterin 0.05 mM: 18% inhibition; 18% inhibition of the reductive reaction at 0.05 mM Equus caballus
acetic acid IC50 0.45 mM, pH 6.5 Equus caballus
benzoic acid 0.03 mM: 40% inhibition; 40% inhibition of the reductive reaction at 0.03 mM Equus caballus
biopterin 0.05 mM: 24% inhibition; 24% inhibition of the reductive reaction at 0.05 mM Equus caballus
biopterin-H2 31% inhibition of the reductive reaction at 0.05 mM Equus caballus
D,L-Lactic acid 0.03 mM, 20% inhibition Equus caballus
D-erythro-neopterin 14% inhibition of the reductive reaction at 0.05 mM Equus caballus
D-erythro-neopterin-H2 7% inhibition of the reductive reaction at 0.05 mM Equus caballus
dihydrobiopterin 0.05 mM: 31% inhibition Equus caballus
dihydrobiopterin
-
 Rattus norvegicus
dihydroxanthopterin slight Equus caballus
DL-lactic acid 20% inhibition of the reductive reaction at 0.03 mM Equus caballus
formic acid slight Equus caballus
iodoacetate 72% inhibition of the reductive reaction at 0.1 mM Equus caballus
Isosepiapterin 83% inhibition of the reductive reaction at 0.05 mM; IC50 0.0065 mM, 0.05 mM: 83% inhibition Equus caballus
Isoxanthopterin 0.05 mM: very slight Equus caballus
KCN 25% inhibition of the reductive reaction at 2.5 mM; slight Equus caballus
L-erythro-dihydroneopterin 0.05 mM: 29% inhibition, D-isomer: slight inhibition Equus caballus
L-Erythro-neopterin 0.05 mM: 21% inhibition, D-isomer: slight inhibition; 21% inhibition of the reductive reaction at 0.05 mM Equus caballus
L-Erythro-neopterin-H2 29% inhibition of the reductive reaction at 0.05 mM Equus caballus
Leucopterin 0.05 mM: 21% inhibition; 21% inhibition of the reductive reaction at 0.05 mM Equus caballus
monoiodoacetate
-
 Equus caballus
additional information other unconjugated pteridines, e.g. xanthopterin, that cause slight inhibition of enzymatic reduction Equus caballus
n-butyric acid IC50 0.032 mM, pH 6.5 Equus caballus
N-ethylmaleimide
-
 Equus caballus
n-valeric acid IC50 0.25 mM, pH 6.5 Equus caballus
NADPH above 0.1 mM Equus caballus
NaN3 15% inhibition of the reductive reaction at 5 mM; slight Equus caballus
NEM 35% inhibition of the reductive reaction at 0.1 mM, 88% at 1 mM Equus caballus
p-chloromercuribenzoate
-
 Equus caballus
PCMB 93% inhibition of the reductive reaction at 0.01 mM Equus caballus
Propionamide 0.03 mM, 14% inhibition; 14% inhibition of the reductive reaction at 0.03 mM Equus caballus
propionic acid 43% inhibition of the reductive reaction at 0.03 mM; IC50 0.0045 mM, pH 6.5 Equus caballus
pterin 0.05 mM: 18% inhibition; 18% inhibition of the reductive reaction at 0.05 mM Equus caballus
Pyruvic acid 9% inhibition of the reductive reaction at 0.03 mM; slight Equus caballus
sepiapterin above 0.05 mM Equus caballus
sepiapterin
-
 Rattus norvegicus
Sodium acetate
-
 Equus caballus
Xanthopterin slight Equus caballus
Xanthopterin B2 slight Equus caballus
Xanthopterin-B2 12% inhibition of the reductive reaction at 0.05 mM Equus caballus
xanthopterin-H2 9% inhibition of the reductive reaction at 0.05 mM Equus caballus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Equus caballus
0.0014
-
 NADPH pH 10.4, 25°C Equus caballus
0.0021
-
 sepiapterin pH 10.4, 25°C Equus caballus
0.0057
-
 NADP+ pH 10.4, 25°C Equus caballus
0.0077
-
 7,8-dihydrobiopterin pH 10.4, 25°C Equus caballus
0.014
-
 NADPH
-
 Equus caballus
0.021
-
 sepiapterin
-
 Equus caballus
0.057
-
 dihydrobiopterin at pH 10.4 Equus caballus
0.077
-
 NADP+ at pH 10.4 Equus caballus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm 92% of total activity Equus caballus 5737
-
additional information intracellular localization, overview Equus caballus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-(S)-lactoyl-7,8-dihydropterin + NADPH Rattus norvegicus equilibrium lies much in favor of dihydrobiopterin formation 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 r
6-(S)-lactoyl-7,8-dihydropterin + NADPH Equus caballus equilibrium lies much in favor of dihydrobiopterin formation 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 r
6-(S)-lactoyl-7,8-dihydropterin + NADPH Rattus norvegicus reverse reaction, L-erythro and L-threo-dihydroneopterin are also oxidized, the corresponding D-isomers are inactive 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 r
6-(S)-lactoyl-7,8-dihydropterin + NADPH Equus caballus reverse reaction, L-erythro and L-threo-dihydroneopterin are also oxidized, the corresponding D-isomers are inactive 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 r
sepiapterin + NADPH + H+ Equus caballus
-
 7,8-dihydrobiopterin + NADP+
-
 r

Organism

Organism UniProt Comment Textmining
Equus caballus
-
-
-
Rattus norvegicus
-
-
-
Rattus norvegicus
-
 female and male Wistar rats
-

Purification (Commentary)

Purification (Comment) Organism
liver Equus caballus
native enzyme 5000fold from liver by ammonium sulfate and protamine sulfate fractionation, and two times treatment by hydroxylapatite and anion exchange chromatography Equus caballus

Source Tissue

Source Tissue Comment Organism Textmining
blood
-
 Rattus norvegicus
-
blood
-
 Equus caballus
-
brain
-
 Rattus norvegicus
-
erythrocyte
-
 Rattus norvegicus
-
erythrocyte
-
 Equus caballus
-
heart
-
 Rattus norvegicus
-
kidney
-
 Rattus norvegicus
-
liver
-
 Rattus norvegicus
-
liver
-
 Equus caballus
-
additional information tissue distribution, no activity in stomach, small intestine, and muscle, overview Rattus norvegicus
-
spleen
-
 Rattus norvegicus
-
testis
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 purified enzyme Equus caballus

Storage Stability

Storage Stability Organism
-15°C, in 150 mM Tris-HCl buffer, pH 6.0, second hydroxylapatite fraction, for 1 week, relatively little loss of activity Equus caballus
-15°C, purified enzyme, 0.01 M potassium phosphate buffer, pH 6.0, stable for 1 week with little loss of activity Equus caballus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-(S)-lactoyl-7,8-dihydropterin + NADPH in the reverse reaction, L-erythro and L-threo-dihydroneopterin are also oxidized, the corresponding L-isomers are inactive Rattus norvegicus 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 r
6-(S)-lactoyl-7,8-dihydropterin + NADPH in the reverse reaction, L-erythro and L-threo-dihydroneopterin are also oxidized, the corresponding L-isomers are inactive Equus caballus 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 r
6-(S)-lactoyl-7,8-dihydropterin + NADPH equilibrium lies much in favor of dihydrobiopterin formation Rattus norvegicus 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 r
6-(S)-lactoyl-7,8-dihydropterin + NADPH equilibrium lies much in favor of dihydrobiopterin formation Equus caballus 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 r
6-(S)-lactoyl-7,8-dihydropterin + NADPH reverse reaction, L-erythro and L-threo-dihydroneopterin are also oxidized, the corresponding D-isomers are inactive Rattus norvegicus 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 r
6-(S)-lactoyl-7,8-dihydropterin + NADPH reverse reaction, L-erythro and L-threo-dihydroneopterin are also oxidized, the corresponding D-isomers are inactive Equus caballus 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 r
isosepiapterin + NADPH
-
 Equus caballus 6-(1-hydroxypropyl)-dihydropterin + NADP+
-
 ?
isosepiapterin + NADPH
-
 Equus caballus ? + NADP+
-
 r
L-erythro-dihydroneopterin + NADP+ D-isomer completely inactive Equus caballus ?
-
 r
L-erythro-dihydroneopterin + NADP+ very slow activity, no activity with the D-isomer Equus caballus ?
-
 r
L-threo-dihydroneopterin + NADP+ D-isomer completely inactive Equus caballus ?
-
 r
L-threo-dihydroneopterin + NADP+ very slow activity, no activity with the D-isomer Equus caballus ?
-
 r
additional information not: 6-acetyl-7-methyldihydropterin Rattus norvegicus ?
-
 ?
additional information not: 6-acetyl-7-methyldihydropterin Equus caballus ?
-
 ?
additional information no activity with 6-acetyl-7-methyl-7,8-dihydropterin Equus caballus ?
-
 ?
sepiapterin + NADPH + H+
-
 Rattus norvegicus 7,8-dihydrobiopterin + NADP+
-
 r
sepiapterin + NADPH + H+
-
 Equus caballus 7,8-dihydrobiopterin + NADP+
-
 r
xanthopterin B2 + NADPH
-
 Equus caballus 6-(1',2'-dihydroxypropyl)-dihydrolumazine + NADP+
-
 ?
xanthopterin-B2 + NADPH
-
 Equus caballus ? + NADP+
-
 r

Synonyms

Synonyms Comment Organism
SR
-
 Rattus norvegicus
SR
-
 Equus caballus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Rattus norvegicus
25
-
 assay at Equus caballus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 in potassium phosphate buffer Rattus norvegicus
5.5
-
 in potassium phosphate buffer Equus caballus
5.5
-
 for reduction of sepiapterin Equus caballus
6.4
-
 substrate reduction reaction, assay at Rattus norvegicus
6.4
-
 substrate reduction reaction, assay at Equus caballus
10.4
-
 the reverse reaction, oxidation of 7,8-dihydrobiopterin, activity rises with rise of pH Equus caballus
10.4
-
 substrate oxidation reaction, assay at Rattus norvegicus
10.4
-
 substrate oxidation reaction, assay at Equus caballus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5
-
 unstable Rattus norvegicus
5.5
-
 unstable Equus caballus
5.5
-
 pH 6.4 for enzyme assay Equus caballus

Cofactor

Cofactor Comment Organism Structure
NAD+ reverse reaction, one-fifth of that with NADP+ Equus caballus
NADH
-
 Rattus norvegicus
NADH activity one-fourth of that with NADPH Equus caballus
NADP+
-
 Rattus norvegicus
NADP+
-
 Equus caballus
NADPH
-
 Rattus norvegicus
NADPH
-
 Equus caballus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0045
-
 IC50 0.0045 mM, pH 6.5 Equus caballus propionic acid
0.0065
-
 IC50 0.0065 mM, 0.05 mM: 83% inhibition Equus caballus Isosepiapterin
0.014
-
 IC50 0.014 mM, 0.05 mM: 77% inhibition Equus caballus 6-Carboxypterin
0.032
-
 IC50 0.032 mM, pH 6.5 Equus caballus n-butyric acid
0.25
-
 IC50 0.25 mM, pH 6.5 Equus caballus n-valeric acid
0.45
-
 IC50 0.45 mM, pH 6.5 Equus caballus acetic acid