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show all sequences of 1.1.1.152

Purification and substrate specificities of bacterial hydroxysteroid dehydrogenases

Roe, C.R.; Kaplan, N.O.; Biochemistry 8, 5093-5103 (1969)

Data extracted from this reference:

Application
Application
Commentary
Organism
analysis
selective microquantitation of steroid substrates
Comamonas testosteroni
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
47000
-
meniscus depletion method, gel filtration
Comamonas testosteroni
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3alpha-hydroxy-5beta-androstane-17-one + NAD+
Comamonas testosteroni
etiocholanolone, strong substrate specificity
5beta-androstane-3,17-dione + NADH
-
Comamonas testosteroni
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Comamonas testosteroni
-
2 strains, ATCC 11996 and STDH-m
-
Purification (Commentary)
Commentary
Organism
to homogeneity, chromatography techniques, preparative gel electrophoresis
Comamonas testosteroni
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Comamonas testosteroni
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3alpha-hydroxy-5beta-androstane-17-one + NAD+
etiocholanolone, strong substrate specificity
286009
Comamonas testosteroni
5beta-androstane-3,17-dione + NADH
-
286009
Comamonas testosteroni
ir
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Comamonas testosteroni
Application (protein specific)
Application
Commentary
Organism
analysis
selective microquantitation of steroid substrates
Comamonas testosteroni
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Comamonas testosteroni
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
47000
-
meniscus depletion method, gel filtration
Comamonas testosteroni
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3alpha-hydroxy-5beta-androstane-17-one + NAD+
Comamonas testosteroni
etiocholanolone, strong substrate specificity
5beta-androstane-3,17-dione + NADH
-
Comamonas testosteroni
ir
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity, chromatography techniques, preparative gel electrophoresis
Comamonas testosteroni
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Comamonas testosteroni
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3alpha-hydroxy-5beta-androstane-17-one + NAD+
etiocholanolone, strong substrate specificity
286009
Comamonas testosteroni
5beta-androstane-3,17-dione + NADH
-
286009
Comamonas testosteroni
ir
Other publictions for EC 1.1.1.152
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
286009
Roe
Purification and substrate spe ...
Comamonas testosteroni
Biochemistry
8
5093-5103
1969
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