BRENDA - Enzyme Database show
show all sequences of 1.1.1.151

The 21-hydroxysteroid dehydrogenases of liver. A nicotinamide adenine dinucleotide phosphate dehydrogenase and two nicotinamide adenine dinucleotide dehydrogenases

Monder, C.; White, A.; J. Biol. Chem. 240, 71-77 (1965)

Data extracted from this reference:

General Stability
General Stability
Organism
not sensitive against repeated freezing and thawing
Ovis aries aries
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ag+
complete inhibition at 0.004 mM
Ovis aries aries
Hg2+
53% inhibition at 0.002 mM
Ovis aries aries
NADH
competitive inhibition
Ovis aries aries
NADPH
competitive inhibition
Ovis aries aries
p-mercuribenzoate
55% inhibition at 0.014 mM
Ovis aries aries
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.021
-
NADPH
-
Ovis aries aries
0.118
-
pregn-4-ene-3,20,21-trione
-
Ovis aries aries
0.137
-
11beta,17-dihydroxy-pregn-4,11-diene-3,20,21-trione
-
Ovis aries aries
0.137
-
17-hydroxy-pregn-4-ene-3,20,21-trione
-
Ovis aries aries
0.177
-
11beta,17-dihydroxy-pregn-4-ene-3,20,21-trione
-
Ovis aries aries
0.233
-
11beta-hydroxy-pregn-4-ene-3,20,21-trione
-
Ovis aries aries
0.461
-
17-hydroxy-pregn-4-ene-3,11,20,21-tetraone
-
Ovis aries aries
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ovis aries aries
-
sheep
-
Purification (Commentary)
Commentary
Organism
to homogeneity, Sephadex G-100, starch block elelctrophoresis
Ovis aries aries
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Ovis aries aries
-
Storage Stability
Storage Stability
Organism
-10C, 0.01 M sodium phosphate , pH 6-7.4, 1 year
Ovis aries aries
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
11beta,17-dihydroxy-pregn-4,11-diene-3,20,21-trione + NADPH
i.e. 21-dehydro-delta1-cortisol
286004
Ovis aries aries
11beta,17,21-trihydroxy-pregn-4,11-diene-3,20-dione + NADP+
21-hydroxy-delta1-cortisol
286004
Ovis aries aries
?
11beta,17-dihydroxy-pregn-4-ene-3,20,21-trione + NADPH
i.e. 21-dehydrocortisol
286004
Ovis aries aries
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+
i.e. cortisol
286004
Ovis aries aries
?
11beta-hydroxy-pregn-4-ene-3,20,21-trione + NADPH
i.e. 21-dehydrocorticosterone
286004
Ovis aries aries
11beta,21-dihydroxy-pregn-4-ene-3,20-dione + NADP+
21-hydroxycorticosterone
286004
Ovis aries aries
?
17-hydroxy-pregn-4-ene-3,20,21-trione + NADPH
i.e. 21-dehydro-11-deoxycortisol
286004
Ovis aries aries
17,21-dihydroxy-pregn-4-ene-3,20-dione + NADP+
i.e. 21-hydroxy-11-deoxycortisol
286004
Ovis aries aries
?
17alpha-hydroxy-pregn-4-ene-3,11,20,21-tetraone + NADPH
i.e. 21-dehydrocortisone, marked dependence on the nature of steroids
286004
Ovis aries aries
17alpha,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADP+
i.e. cortisone
286004
Ovis aries aries
?
pregn-4-ene-3,20,21-trione + NADPH
i.e. 21-dehydro-11-deoxy-corticosterone
286004
Ovis aries aries
21-hydroxy-pregn-4-ene-3,20-dione + NADP+
21-hydroxy-11-deoxy-corticosterone
286004
Ovis aries aries
?
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
56
-
80% loss of activity in 5 min
Ovis aries aries
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.9
6.1
-
Ovis aries aries
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Ovis aries aries
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Ovis aries aries
General Stability (protein specific)
General Stability
Organism
not sensitive against repeated freezing and thawing
Ovis aries aries
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ag+
complete inhibition at 0.004 mM
Ovis aries aries
Hg2+
53% inhibition at 0.002 mM
Ovis aries aries
NADH
competitive inhibition
Ovis aries aries
NADPH
competitive inhibition
Ovis aries aries
p-mercuribenzoate
55% inhibition at 0.014 mM
Ovis aries aries
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.021
-
NADPH
-
Ovis aries aries
0.118
-
pregn-4-ene-3,20,21-trione
-
Ovis aries aries
0.137
-
11beta,17-dihydroxy-pregn-4,11-diene-3,20,21-trione
-
Ovis aries aries
0.137
-
17-hydroxy-pregn-4-ene-3,20,21-trione
-
Ovis aries aries
0.177
-
11beta,17-dihydroxy-pregn-4-ene-3,20,21-trione
-
Ovis aries aries
0.233
-
11beta-hydroxy-pregn-4-ene-3,20,21-trione
-
Ovis aries aries
0.461
-
17-hydroxy-pregn-4-ene-3,11,20,21-tetraone
-
Ovis aries aries
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity, Sephadex G-100, starch block elelctrophoresis
Ovis aries aries
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Ovis aries aries
-
Storage Stability (protein specific)
Storage Stability
Organism
-10C, 0.01 M sodium phosphate , pH 6-7.4, 1 year
Ovis aries aries
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
11beta,17-dihydroxy-pregn-4,11-diene-3,20,21-trione + NADPH
i.e. 21-dehydro-delta1-cortisol
286004
Ovis aries aries
11beta,17,21-trihydroxy-pregn-4,11-diene-3,20-dione + NADP+
21-hydroxy-delta1-cortisol
286004
Ovis aries aries
?
11beta,17-dihydroxy-pregn-4-ene-3,20,21-trione + NADPH
i.e. 21-dehydrocortisol
286004
Ovis aries aries
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+
i.e. cortisol
286004
Ovis aries aries
?
11beta-hydroxy-pregn-4-ene-3,20,21-trione + NADPH
i.e. 21-dehydrocorticosterone
286004
Ovis aries aries
11beta,21-dihydroxy-pregn-4-ene-3,20-dione + NADP+
21-hydroxycorticosterone
286004
Ovis aries aries
?
17-hydroxy-pregn-4-ene-3,20,21-trione + NADPH
i.e. 21-dehydro-11-deoxycortisol
286004
Ovis aries aries
17,21-dihydroxy-pregn-4-ene-3,20-dione + NADP+
i.e. 21-hydroxy-11-deoxycortisol
286004
Ovis aries aries
?
17alpha-hydroxy-pregn-4-ene-3,11,20,21-tetraone + NADPH
i.e. 21-dehydrocortisone, marked dependence on the nature of steroids
286004
Ovis aries aries
17alpha,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADP+
i.e. cortisone
286004
Ovis aries aries
?
pregn-4-ene-3,20,21-trione + NADPH
i.e. 21-dehydro-11-deoxy-corticosterone
286004
Ovis aries aries
21-hydroxy-pregn-4-ene-3,20-dione + NADP+
21-hydroxy-11-deoxy-corticosterone
286004
Ovis aries aries
?
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
56
-
80% loss of activity in 5 min
Ovis aries aries
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.9
6.1
-
Ovis aries aries
Other publictions for EC 1.1.1.151
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
286004
Monder
The 21-hydroxysteroid dehydrog ...
Ovis aries aries
J. Biol. Chem.
240
71-77
1965
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1
5
7
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1
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1
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1
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1
6
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1
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1
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1
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1
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1
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5
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7
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1
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1
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1
6
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1
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1
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