BRENDA - Enzyme Database show
show all sequences of 1.1.1.150

The 21-hydroxysteroid dehydrogenases of liver. A nicotinamide adenine dinucleotide phosphate dehydrogenase and two nicotinamide adenine dinucleotide dehydrogenases

Monder, C.; White, A.; J. Biol. Chem. 240, 71-77 (1965)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Ag+
complete inhibition of NAD dehydrogenase II at 0.018 mM
Ovis aries aries
Hg2+
40% inhibition of NAD dehydrogenase II at 0.004 mM
Ovis aries aries
NAD+
NAD dehydrogenase II, competitive inhibition
Ovis aries aries
NADP+
NAD dehydrogenase II, competitive inhibition
Ovis aries aries
p-mercuribenzoate
79% inhibition of NAD dehydrogenase II at 0.014 mM
Ovis aries aries
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
NADH
NAD dehydrogenase I
Ovis aries aries
0.048
-
NADH
NAD dehydrogenase II
Ovis aries aries
0.105
-
11beta-hydroxy-pregn-4-ene-3,20,21-trione
NAD dehydrogenase II
Ovis aries aries
0.111
-
17-hydroxy-pregn-4-ene-3,20,21-trione
NAD dehydrogenase II
Ovis aries aries
0.172
-
11beta,17-dihydroxy-pregn-4,11-diene-3,20,21-trione
NAD dehydrogenase II
Ovis aries aries
0.174
-
pregn-4-ene-3,20,21-trione
NAD dehydrogenase II
Ovis aries aries
0.182
-
11beta,17-dihydroxy-pregn-4-ene-3,20,21-trione
-
Ovis aries aries
0.221
-
17-hydroxy-pregn-4-ene-3,11,20,21-tetraone
-
Ovis aries aries
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ovis aries aries
-
sheep
-
Purification (Commentary)
Commentary
Organism
to homogeneity, gel filtration, starch block electrophoresis
Ovis aries aries
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
2 isoenzymes: NAD dehydrogenase I and II
Ovis aries aries
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
11beta,17-dihydroxy-pregn-4,11-diene-3,20,21-trione + NADH
i.e. 21-dehydro-delta1-cortisol, only NAD dehydrogenase II
286004
Ovis aries aries
11beta,17,21-trihydroxy-pregn-4,11-diene-3,20-dione + NAD+
i.e. 21-hydroxy-delta1-cortisol
286004
Ovis aries aries
?
11beta,17-dihydroxy-pregn-4-ene-3,20,21-trione + NADH
-
286004
Ovis aries aries
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NAD+
i.e. cortisol
286004
Ovis aries aries
?
11beta-hydroxy-pregn-4-ene-3,20,21-trione + NADH
i.e. 21-dehydrocorticosterone, only NAD dehydrogenase II
286004
Ovis aries aries
11beta,21-dihydroxy-pregn-4-ene-3,20-dione + NAD+
i.e. 21-hydroxycorticosterone
286004
Ovis aries aries
?
17-hydroxy-pregn-4-ene-3,11,20,21-tetraone + NADH
i.e. 21-dehydrocortisone
286004
Ovis aries aries
17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NAD+
i.e. cortisone
286004
Ovis aries aries
?
17-hydroxy-pregn-4-ene-3,20,21-trione + NADH
i.e. 21-dehydro-11-deoxycortisol, only NAD dehydrogenase II
286004
Ovis aries aries
17,21-dihydroxy-pregn-4-ene-3,20-dione + NAD+
i.e. 21-hydroxy-11-deoxycortisol
286004
Ovis aries aries
?
pregn-4-ene-3,20,21-trione + NADH
i.e. 21-dehydro-11-deoxycorticosterone, only NAD dehydrogenase II
286004
Ovis aries aries
21-hydroxy-pregn-4-ene-3,20-dione + NAD+
i.e. 21-hydroxy-11-deoxycorticosterone
286004
Ovis aries aries
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
56
-
30% loss of activity in 5 min, NAD dehydrogenase I more heat resistant than NAD dehydrogenase II
Ovis aries aries
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.2
-
NAD dehydrogenase II
Ovis aries aries
6.9
-
NAD dehydrogenase I
Ovis aries aries
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Ovis aries aries
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Ovis aries aries
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ag+
complete inhibition of NAD dehydrogenase II at 0.018 mM
Ovis aries aries
Hg2+
40% inhibition of NAD dehydrogenase II at 0.004 mM
Ovis aries aries
NAD+
NAD dehydrogenase II, competitive inhibition
Ovis aries aries
NADP+
NAD dehydrogenase II, competitive inhibition
Ovis aries aries
p-mercuribenzoate
79% inhibition of NAD dehydrogenase II at 0.014 mM
Ovis aries aries
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
NADH
NAD dehydrogenase I
Ovis aries aries
0.048
-
NADH
NAD dehydrogenase II
Ovis aries aries
0.105
-
11beta-hydroxy-pregn-4-ene-3,20,21-trione
NAD dehydrogenase II
Ovis aries aries
0.111
-
17-hydroxy-pregn-4-ene-3,20,21-trione
NAD dehydrogenase II
Ovis aries aries
0.172
-
11beta,17-dihydroxy-pregn-4,11-diene-3,20,21-trione
NAD dehydrogenase II
Ovis aries aries
0.174
-
pregn-4-ene-3,20,21-trione
NAD dehydrogenase II
Ovis aries aries
0.182
-
11beta,17-dihydroxy-pregn-4-ene-3,20,21-trione
-
Ovis aries aries
0.221
-
17-hydroxy-pregn-4-ene-3,11,20,21-tetraone
-
Ovis aries aries
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity, gel filtration, starch block electrophoresis
Ovis aries aries
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
2 isoenzymes: NAD dehydrogenase I and II
Ovis aries aries
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
11beta,17-dihydroxy-pregn-4,11-diene-3,20,21-trione + NADH
i.e. 21-dehydro-delta1-cortisol, only NAD dehydrogenase II
286004
Ovis aries aries
11beta,17,21-trihydroxy-pregn-4,11-diene-3,20-dione + NAD+
i.e. 21-hydroxy-delta1-cortisol
286004
Ovis aries aries
?
11beta,17-dihydroxy-pregn-4-ene-3,20,21-trione + NADH
-
286004
Ovis aries aries
11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NAD+
i.e. cortisol
286004
Ovis aries aries
?
11beta-hydroxy-pregn-4-ene-3,20,21-trione + NADH
i.e. 21-dehydrocorticosterone, only NAD dehydrogenase II
286004
Ovis aries aries
11beta,21-dihydroxy-pregn-4-ene-3,20-dione + NAD+
i.e. 21-hydroxycorticosterone
286004
Ovis aries aries
?
17-hydroxy-pregn-4-ene-3,11,20,21-tetraone + NADH
i.e. 21-dehydrocortisone
286004
Ovis aries aries
17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NAD+
i.e. cortisone
286004
Ovis aries aries
?
17-hydroxy-pregn-4-ene-3,20,21-trione + NADH
i.e. 21-dehydro-11-deoxycortisol, only NAD dehydrogenase II
286004
Ovis aries aries
17,21-dihydroxy-pregn-4-ene-3,20-dione + NAD+
i.e. 21-hydroxy-11-deoxycortisol
286004
Ovis aries aries
?
pregn-4-ene-3,20,21-trione + NADH
i.e. 21-dehydro-11-deoxycorticosterone, only NAD dehydrogenase II
286004
Ovis aries aries
21-hydroxy-pregn-4-ene-3,20-dione + NAD+
i.e. 21-hydroxy-11-deoxycorticosterone
286004
Ovis aries aries
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
56
-
30% loss of activity in 5 min, NAD dehydrogenase I more heat resistant than NAD dehydrogenase II
Ovis aries aries
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.2
-
NAD dehydrogenase II
Ovis aries aries
6.9
-
NAD dehydrogenase I
Ovis aries aries
Other publictions for EC 1.1.1.150
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
688677
Keller
RDH12, a retinol dehydrogenase ...
Homo sapiens, Mus musculus
J. Steroid Biochem. Mol. Biol.
104
190-194
2007
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2
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3
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2
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2
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2
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3
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2
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286007
Holland
-
Stereochemistry of hydrogen lo ...
Bos taurus, Eggerthella lenta
Can. J. Chem.
67
1590-1595
1989
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2
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1
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2
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1
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2
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286005
Orr
The stereospecificity of the e ...
Ovis aries aries
J. Biol. Chem.
250
7547-7553
1975
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1
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1
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1
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1
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1
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1
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1
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286006
Orr
The stereochemistry of enzymat ...
Ovis aries aries
J. Steroid Biochem.
6
297-299
1975
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1
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1
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1
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1
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1
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1
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1
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286004
Monder
The 21-hydroxysteroid dehydrog ...
Ovis aries aries
J. Biol. Chem.
240
71-77
1965
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-
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5
8
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1
-
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1
-
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1
-
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6
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1
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2
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1
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1
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5
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8
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1
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1
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6
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1
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2
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