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Literature summary for 1.1.1.15 extracted from

  • Fredslund, F.; Otten, H.; Gemperlein, S.; Poulsen, J.C.; Carius, Y.; Kohring, G.W.; Lo Leggio, L.
    Structural characterization of the thermostable Bradyrhizobium japonicum D-sorbitol dehydrogenase (2016), Acta Crystallogr. Sect. F, 72, 846-852.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.15

Cloned(Commentary)

Cloned (Comment) Organism
gene rdh, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-Gold (DE3) Bradyrhizobium japonicum

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His-tagged enzyme in complex with both NAD+ and glucitol, X-ray diffraction structure determination and analysis at 2.9 A resolution, molecular replacement and modelling Bradyrhizobium japonicum

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc metalloenzyme Bradyrhizobium japonicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26900
-
 SDS-PAGE, subunit Bradyrhizobium japonicum
77700
-
 native PAGE, recombinant enzyme Bradyrhizobium japonicum
83200
-
 gel filtration, recombinant enzyme Bradyrhizobium japonicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-iditol + NAD+ Bradyrhizobium japonicum i.e. D-sorbitol D-sorbose + NADH + H+
-
 r
D-iditol + NAD+ Bradyrhizobium japonicum USDA110 i.e. D-sorbitol D-sorbose + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Bradyrhizobium japonicum Q89FN7 i.e. Bradyrhizobium diazoefficiens
-
Bradyrhizobium japonicum USDA110 Q89FN7 i.e. Bradyrhizobium diazoefficiens
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21-Gold (DE3) by heat treatment at 50°C and metal affinity chromatography to homogeneity Bradyrhizobium japonicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucitol + NAD+
-
 Bradyrhizobium japonicum D-fructose + NADH + H+
-
 r
D-glucitol + NAD+
-
 Bradyrhizobium japonicum USDA110 D-fructose + NADH + H+
-
 r
D-iditol + NAD+ i.e. D-sorbitol Bradyrhizobium japonicum D-sorbose + NADH + H+
-
 r
D-iditol + NAD+ i.e D-sorbitol Bradyrhizobium japonicum D-sorbose + NADH + H+
-
 r
D-iditol + NAD+ i.e. D-sorbitol Bradyrhizobium japonicum USDA110 D-sorbose + NADH + H+
-
 r
D-iditol + NAD+ i.e D-sorbitol Bradyrhizobium japonicum USDA110 D-sorbose + NADH + H+
-
 r
L-glucitol + NAD+ i.e. D-sorbitol, 90% conversion of L-glucitol to D-sorbose Bradyrhizobium japonicum D-sorbose + NADH + H+
-
 r
L-glucitol + NAD+ i.e. D-sorbitol, 90% conversion of L-glucitol to D-sorbose Bradyrhizobium japonicum USDA110 D-sorbose + NADH + H+
-
 r
additional information the substrate in BjSDH is stabilized via the side chain of Glu150, Ser140 and the carbonyl groups of Pro183 and Gly184 Bradyrhizobium japonicum ?
-
 ?
additional information the substrate in BjSDH is stabilized via the side chain of Glu150, Ser140 and the carbonyl groups of Pro183 and Gly184 Bradyrhizobium japonicum USDA110 ?
-
 ?

Subunits

Subunits Comment Organism
More a continuous beta-sheet is formed between two monomers in the tetramer. The BjSDH structure folds in the Rossmann fold. The monomer of BjSDH is composed of a central parallel beta-sheet surrounded by three alpha-helices on each side. At the top of the beta-sheet a small helix–turn–helix motif is located between the two final beta-strands betaF and betaG. Tetrameric quaternary organization, structure comparisons, overview Bradyrhizobium japonicum
tetramer 4 * 26900, SDS-PAGE and crystal structure analysis Bradyrhizobium japonicum

Synonyms

Synonyms Comment Organism
BjSDH
-
 Bradyrhizobium japonicum
D-sorbitol dehydrogenase
-
 Bradyrhizobium japonicum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Bradyrhizobium japonicum
NADH dependent on Bradyrhizobium japonicum

General Information

General Information Comment Organism
evolution the enzyme BjSDH is part of the superfamily of Zn-independent short-chain dehydrogenases. The BjSDH structure folds in the Rossmann fold, which is typical for NADH-dependent enzymes and the SDR family Bradyrhizobium japonicum