BRENDA - Enzyme Database show
show all sequences of 1.1.1.148

Characterization of 17alpha-hydroxysteroid dehydrogenase activity (17alpha-HSD) and its involvement in the biosynthesis of epitestosterone

Bellemare, V.; Faucher, F.; Breton, R.; Luu-The, V.; BMC Biochem. 6, 12 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
cDNA cloning, subcloning in Escherichia coli, expression as GST-fusion protein in Escherichia coli strain BL21(DE3), stable expression in HEK-293 cells
Mus musculus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Mus musculus
the enzyme is involved in the biosynthesis of epitestosterone, the 17alpha-epimer of testosterone
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mus musculus
Q91WR5
-
-
Purification (Commentary)
Commentary
Organism
recombinant GST-fusion enzyme from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography
Mus musculus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
high expression level
Mus musculus
-
additional information
enzyme tissue distribution in male and female mice, overview
Mus musculus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5alpha-dihydrotestosterone + NADPH
-
668078
Mus musculus
5alpha-androstane-3alpha,17alpha-diol + NADP+
-
-
-
?
5alpha-pregnane-3,20-dione + NADPH + H+
-
668078
Mus musculus
3alpha,5alpha-3-hydroxypregnan-20-one + NADP+
-
-
-
?
androstanedione + NADPH
-
668078
Mus musculus
5alpha-androstane-3-one-17alpha-ol + NADP+
-
-
-
?
androstenedione + NADPH + H+
-
668078
Mus musculus
epitestosterone + NADP+
-
-
-
?
androsterone + NADPH
-
668078
Mus musculus
5alpha-androstane-3alpha,17alpha-diol + NADP+
-
-
-
?
dehydroepiandrosterone + NADPH
-
668078
Mus musculus
5-androstene-3alpha,17alpha-diol + NADP+
-
-
-
?
additional information
the enzyme is involved in the biosynthesis of epitestosterone, the 17alpha-epimer of testosterone
668078
Mus musculus
?
-
-
-
-
additional information
substrate specificity of the recombinant enzyme, overview
668078
Mus musculus
?
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3
-
assay at
Mus musculus
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Mus musculus
Cloned(Commentary) (protein specific)
Commentary
Organism
cDNA cloning, subcloning in Escherichia coli, expression as GST-fusion protein in Escherichia coli strain BL21(DE3), stable expression in HEK-293 cells
Mus musculus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Mus musculus
the enzyme is involved in the biosynthesis of epitestosterone, the 17alpha-epimer of testosterone
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant GST-fusion enzyme from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography
Mus musculus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
high expression level
Mus musculus
-
additional information
enzyme tissue distribution in male and female mice, overview
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5alpha-dihydrotestosterone + NADPH
-
668078
Mus musculus
5alpha-androstane-3alpha,17alpha-diol + NADP+
-
-
-
?
5alpha-pregnane-3,20-dione + NADPH + H+
-
668078
Mus musculus
3alpha,5alpha-3-hydroxypregnan-20-one + NADP+
-
-
-
?
androstanedione + NADPH
-
668078
Mus musculus
5alpha-androstane-3-one-17alpha-ol + NADP+
-
-
-
?
androstenedione + NADPH + H+
-
668078
Mus musculus
epitestosterone + NADP+
-
-
-
?
androsterone + NADPH
-
668078
Mus musculus
5alpha-androstane-3alpha,17alpha-diol + NADP+
-
-
-
?
dehydroepiandrosterone + NADPH
-
668078
Mus musculus
5-androstene-3alpha,17alpha-diol + NADP+
-
-
-
?
additional information
the enzyme is involved in the biosynthesis of epitestosterone, the 17alpha-epimer of testosterone
668078
Mus musculus
?
-
-
-
-
additional information
substrate specificity of the recombinant enzyme, overview
668078
Mus musculus
?
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3
-
assay at
Mus musculus
Other publictions for EC 1.1.1.148
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
668078
Bellemare
Characterization of 17alpha-hy ...
Mus musculus
BMC Biochem.
6
12
2005
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8
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1
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285976
De Prada
Purification and characterizat ...
Eubacterium sp., Eubacterium sp. VPI 12708
J. Lipid Res.
35
922-929
1994
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1
1
4
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2
2
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12
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1
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2
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4
1
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2
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1
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1
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1
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1
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4
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2
2
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1
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2
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4
1
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2
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285970
LaRhee
Purification and characterizat ...
Equus caballus
Biochemistry
23
486-491
1984
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1
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2
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2
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1
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2
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1
1
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1
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2
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1
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1
1
1
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1
1
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285971
Johnston
Oestradiol-17alpha dehydrogena ...
Gallus gallus
Biochem. J.
222
761-768
1984
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1
2
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4
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1
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2
1
1
4
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1
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2
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1
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1
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2
1
1
4
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1
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285972
Hasnain
Properties of the multiple for ...
Oryctolagus cuniculus
Biochem. J.
161
279-283
1977
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1
3
1
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1
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2
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1
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2
5
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5
1
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2
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2
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1
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1
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5
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5
1
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285973
Hasnain
Purification of multiple forms ...
Oryctolagus cuniculus
Biochem. J.
147
457-461
1975
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1
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3
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285975
Hasnain
The separation and partial pur ...
Oryctolagus cuniculus
Can. J. Biochem.
52
120-125
1974
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-
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-
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1
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285974
Renwick
The partial purification of 17 ...
Gallus gallus
Biochim. Biophys. Acta
146
336-348
1967
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1
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