BRENDA - Enzyme Database show
show all sequences of 1.1.1.148

Purification and characterization of a novel 17alpha-hydroxysteroid dehydrogenase from an intestinal Eubacterium sp. VPI 12708

De Prada, P.; Setchell, K.D.R.; Hylemon, P.B.; J. Lipid Res. 35, 922-929 (1994)

Data extracted from this reference:

General Stability
General Stability
Organism
sensitive to molecular oxygen
Eubacterium sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
-
Eubacterium sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
NADP+
-
Eubacterium sp.
0.005
-
NADPH
-
Eubacterium sp.
0.031
-
4-androsten-3,17-dione
-
Eubacterium sp.
0.07
-
Epitestosterone
-
Eubacterium sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Eubacterium sp.
Zn2+
-
Eubacterium sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
4 * 42000, SDS-PAGE
Eubacterium sp.
160000
-
gel filtration
Eubacterium sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Eubacterium sp.
-
inducible by growth on androstenedione and cholic acid, not deoxycholic acid; VPI 12708
-
Eubacterium sp. VPI 12708
-
VPI 12708
-
Purification (Commentary)
Commentary
Organism
-
Eubacterium sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.8
-
oxidation of 17alpha-hydroxy-4-androsten-3-one
Eubacterium sp.
3.25
-
reduction of 4-androsten-3,17-dione
Eubacterium sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
17alpha-estradiol + NAD(P)+
-
285976
Eubacterium sp.
estrone + NAD(P)H
-
-
-
r
17alpha-estradiol + NAD(P)+
-
285976
Eubacterium sp. VPI 12708
estrone + NAD(P)H
-
-
-
r
17alpha-hydroxy-4-androsten-3-one + NADP+
epitestosterone
285976
Eubacterium sp.
4-androsten-3,17-dione + NADPH
-
-
-
r
17alpha-hydroxy-4-androsten-3-one + NADP+
epitestosterone
285976
Eubacterium sp. VPI 12708
4-androsten-3,17-dione + NADPH
-
-
-
r
Subunits
Subunits
Commentary
Organism
tetramer
4 * 42000, SDS-PAGE
Eubacterium sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
7
reduction of 4-androsten-3,17-dione
Eubacterium sp.
6.5
-
oxidation of 17alpha-hydroxy-4-androsten-3-one
Eubacterium sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
highly specific for
Eubacterium sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
highly specific for
Eubacterium sp.
General Stability (protein specific)
General Stability
Organism
sensitive to molecular oxygen
Eubacterium sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
-
Eubacterium sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
NADP+
-
Eubacterium sp.
0.005
-
NADPH
-
Eubacterium sp.
0.031
-
4-androsten-3,17-dione
-
Eubacterium sp.
0.07
-
Epitestosterone
-
Eubacterium sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Eubacterium sp.
Zn2+
-
Eubacterium sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
4 * 42000, SDS-PAGE
Eubacterium sp.
160000
-
gel filtration
Eubacterium sp.
Purification (Commentary) (protein specific)
Commentary
Organism
-
Eubacterium sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.8
-
oxidation of 17alpha-hydroxy-4-androsten-3-one
Eubacterium sp.
3.25
-
reduction of 4-androsten-3,17-dione
Eubacterium sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
17alpha-estradiol + NAD(P)+
-
285976
Eubacterium sp.
estrone + NAD(P)H
-
-
-
r
17alpha-estradiol + NAD(P)+
-
285976
Eubacterium sp. VPI 12708
estrone + NAD(P)H
-
-
-
r
17alpha-hydroxy-4-androsten-3-one + NADP+
epitestosterone
285976
Eubacterium sp.
4-androsten-3,17-dione + NADPH
-
-
-
r
17alpha-hydroxy-4-androsten-3-one + NADP+
epitestosterone
285976
Eubacterium sp. VPI 12708
4-androsten-3,17-dione + NADPH
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 42000, SDS-PAGE
Eubacterium sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
7
reduction of 4-androsten-3,17-dione
Eubacterium sp.
6.5
-
oxidation of 17alpha-hydroxy-4-androsten-3-one
Eubacterium sp.
Other publictions for EC 1.1.1.148
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
668078
Bellemare
Characterization of 17alpha-hy ...
Mus musculus
BMC Biochem.
6
12
2005
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1
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1
-
4
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1
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2
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8
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1
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1
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1
1
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1
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1
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2
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8
-
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-
1
-
-
-
-
-
-
-
-
-
285976
De Prada
Purification and characterizat ...
Eubacterium sp., Eubacterium sp. VPI 12708
J. Lipid Res.
35
922-929
1994
-
-
-
-
-
1
1
4
-
2
2
-
-
12
-
-
1
-
-
-
2
-
4
1
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-
-
2
-
-
1
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-
1
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1
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1
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4
-
2
2
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1
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2
-
4
1
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-
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-
2
-
-
-
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-
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-
285970
LaRhee
Purification and characterizat ...
Equus caballus
Biochemistry
23
486-491
1984
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-
-
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1
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2
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2
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1
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2
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1
1
1
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1
1
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2
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2
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1
-
-
2
-
-
-
-
1
-
2
-
1
1
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
285971
Johnston
Oestradiol-17alpha dehydrogena ...
Gallus gallus
Biochem. J.
222
761-768
1984
-
-
-
-
-
-
1
2
-
-
-
-
-
4
-
-
1
-
-
2
1
1
4
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
2
-
-
-
-
-
-
-
1
-
2
1
1
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
285972
Hasnain
Properties of the multiple for ...
Oryctolagus cuniculus
Biochem. J.
161
279-283
1977
-
-
-
-
-
-
1
3
1
-
1
-
-
2
-
-
1
-
-
2
5
-
5
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
3
1
-
1
-
-
-
-
1
-
2
5
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285973
Hasnain
Purification of multiple forms ...
Oryctolagus cuniculus
Biochem. J.
147
457-461
1975
-
-
-
-
-
-
-
-
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2
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1
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2
1
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3
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1
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2
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285975
Hasnain
The separation and partial pur ...
Oryctolagus cuniculus
Can. J. Biochem.
52
120-125
1974
-
-
-
-
-
-
-
-
-
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1
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1
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1
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2
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2
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2
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1
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1
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2
-
-
-
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-
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-
-
-
-
-
-
-
-
-
285974
Renwick
The partial purification of 17 ...
Gallus gallus
Biochim. Biophys. Acta
146
336-348
1967
-
-
-
-
-
-
-
1
-
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-
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1
-
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1
-
-
1
-
1
1
-
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-
-
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1
-
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3
-
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-
-
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3
-
-
-
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-
-
1
-
-
-
-
-
-
-
1
-
1
-
1
1
-
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-
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-
1
-
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