BRENDA - Enzyme Database show
show all sequences of 1.1.1.148

Properties of the multiple forms of the soluble 17alpha-hydroxy steroid dehydrogenase of rabbit liver

Hasnain, S.; Williamson, D.G.; Biochem. J. 161, 279-283 (1977)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Epitestosterone
substrate inhibition for all isoforms at concentration above 0.003 mM, not observed with other substrates
Oryctolagus cuniculus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0002
0.0052
Epitestosterone
for six isoforms
Oryctolagus cuniculus
0.001
0.0051
17alpha-estradiol 3-glucuronide
for six isoforms
Oryctolagus cuniculus
0.0024
0.0088
17alpha-estradiol
for six isoforms
Oryctolagus cuniculus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39600
-
x * 39600, SDS-PAGE, all 8 forms
Oryctolagus cuniculus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Oryctolagus cuniculus
-
eight isoforms
-
Purification (Commentary)
Commentary
Organism
8 forms, six of them purified to homogenity: IA, IB, IC, IIC, IID, III
Oryctolagus cuniculus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.003
0.009
isoform IA, IB, IID, III, 17alpha-estradiol 3-glucuronide
Oryctolagus cuniculus
0.007
0.015
six isoforms, 17alpha-estradiol
Oryctolagus cuniculus
0.041
0.2
six isoforms, epitestosterone
Oryctolagus cuniculus
0.047
-
isoform IIC, 17alpha-estradiol 3-glucuronide
Oryctolagus cuniculus
0.18
-
isoform IC, 17alpha-estradiol 3-glucuronide
Oryctolagus cuniculus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
17alpha-estradiol + NAD(P)+
-
285972
Oryctolagus cuniculus
estrone + NAD(P)H
-
-
-
?
17alpha-hydroxy-4-androsten-3-one + NADP+
epitestosterone
285972
Oryctolagus cuniculus
4-androsten-3,17-dione + NADPH
-
-
-
r
estradiol-17alpha 3-galacturonide + NADP+
-
285972
Oryctolagus cuniculus
estrone 3-galacturonide + NADPH
-
-
-
?
estradiol-17alpha 3-glucoside + NADP+
-
285972
Oryctolagus cuniculus
estrone 3-glucoside + NADPH
-
-
-
?
estradiol-17alpha 3-glucuronide + NADP+
-
285972
Oryctolagus cuniculus
estrone 3-glucuronide + NADPH
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 39600, SDS-PAGE, all 8 forms
Oryctolagus cuniculus
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Oryctolagus cuniculus
NADP+
-
Oryctolagus cuniculus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Oryctolagus cuniculus
NADP+
-
Oryctolagus cuniculus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Epitestosterone
substrate inhibition for all isoforms at concentration above 0.003 mM, not observed with other substrates
Oryctolagus cuniculus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0002
0.0052
Epitestosterone
for six isoforms
Oryctolagus cuniculus
0.001
0.0051
17alpha-estradiol 3-glucuronide
for six isoforms
Oryctolagus cuniculus
0.0024
0.0088
17alpha-estradiol
for six isoforms
Oryctolagus cuniculus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39600
-
x * 39600, SDS-PAGE, all 8 forms
Oryctolagus cuniculus
Purification (Commentary) (protein specific)
Commentary
Organism
8 forms, six of them purified to homogenity: IA, IB, IC, IIC, IID, III
Oryctolagus cuniculus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.003
0.009
isoform IA, IB, IID, III, 17alpha-estradiol 3-glucuronide
Oryctolagus cuniculus
0.007
0.015
six isoforms, 17alpha-estradiol
Oryctolagus cuniculus
0.041
0.2
six isoforms, epitestosterone
Oryctolagus cuniculus
0.047
-
isoform IIC, 17alpha-estradiol 3-glucuronide
Oryctolagus cuniculus
0.18
-
isoform IC, 17alpha-estradiol 3-glucuronide
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
17alpha-estradiol + NAD(P)+
-
285972
Oryctolagus cuniculus
estrone + NAD(P)H
-
-
-
?
17alpha-hydroxy-4-androsten-3-one + NADP+
epitestosterone
285972
Oryctolagus cuniculus
4-androsten-3,17-dione + NADPH
-
-
-
r
estradiol-17alpha 3-galacturonide + NADP+
-
285972
Oryctolagus cuniculus
estrone 3-galacturonide + NADPH
-
-
-
?
estradiol-17alpha 3-glucoside + NADP+
-
285972
Oryctolagus cuniculus
estrone 3-glucoside + NADPH
-
-
-
?
estradiol-17alpha 3-glucuronide + NADP+
-
285972
Oryctolagus cuniculus
estrone 3-glucuronide + NADPH
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 39600, SDS-PAGE, all 8 forms
Oryctolagus cuniculus
Other publictions for EC 1.1.1.148
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
668078
Bellemare
Characterization of 17alpha-hy ...
Mus musculus
BMC Biochem.
6
12
2005
-
-
1
-
-
-
-
-
-
-
-
1
-
4
-
-
1
-
-
2
-
-
8
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
2
-
-
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
285976
De Prada
Purification and characterizat ...
Eubacterium sp., Eubacterium sp. VPI 12708
J. Lipid Res.
35
922-929
1994
-
-
-
-
-
1
1
4
-
2
2
-
-
12
-
-
1
-
-
-
2
-
4
1
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
1
-
1
-
4
-
2
2
-
-
-
-
1
-
-
2
-
4
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
285970
LaRhee
Purification and characterizat ...
Equus caballus
Biochemistry
23
486-491
1984
-
-
-
-
-
-
-
1
-
-
2
-
-
2
-
-
1
-
-
2
-
1
1
1
-
-
-
-
1
1
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
2
-
-
-
-
1
-
2
-
1
1
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
285971
Johnston
Oestradiol-17alpha dehydrogena ...
Gallus gallus
Biochem. J.
222
761-768
1984
-
-
-
-
-
-
1
2
-
-
-
-
-
4
-
-
1
-
-
2
1
1
4
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
2
-
-
-
-
-
-
-
1
-
2
1
1
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
285972
Hasnain
Properties of the multiple for ...
Oryctolagus cuniculus
Biochem. J.
161
279-283
1977
-
-
-
-
-
-
1
3
1
-
1
-
-
2
-
-
1
-
-
2
5
-
5
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
3
1
-
1
-
-
-
-
1
-
2
5
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285973
Hasnain
Purification of multiple forms ...
Oryctolagus cuniculus
Biochem. J.
147
457-461
1975
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
2
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285975
Hasnain
The separation and partial pur ...
Oryctolagus cuniculus
Can. J. Biochem.
52
120-125
1974
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285974
Renwick
The partial purification of 17 ...
Gallus gallus
Biochim. Biophys. Acta
146
336-348
1967
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
1
-
1
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-