BRENDA - Enzyme Database show
show all sequences of 1.1.1.148

Purification and characterization of epimeric estradiol dehydrogenases (17alpha and 17beta) from equine placenta

LaRhee, L.H.; Warren, J.C.; Biochemistry 23, 486-491 (1984)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0017
-
17alpha-estradiol
-
Equus caballus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
x * 33000, SDS-PAGE
Equus caballus
52500
-
gel filtration
Equus caballus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Equus caballus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Equus caballus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
placenta
-
Equus caballus
-
Storage Stability
Storage Stability
Organism
4C, phosphate buffer, pH 7.0, 1 mM dithiothreitol, at least 1 month
Equus caballus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
17alpha-estradiol + NAD(P)+
-
285970
Equus caballus
estrone + NAD(P)H
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 33000, SDS-PAGE
Equus caballus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
9.5
-
Equus caballus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
8
10
-
Equus caballus
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
preferred
Equus caballus
NADP+
40% of NAD+ activity
Equus caballus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
preferred
Equus caballus
NADP+
40% of NAD+ activity
Equus caballus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0017
-
17alpha-estradiol
-
Equus caballus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
x * 33000, SDS-PAGE
Equus caballus
52500
-
gel filtration
Equus caballus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Equus caballus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
placenta
-
Equus caballus
-
Storage Stability (protein specific)
Storage Stability
Organism
4C, phosphate buffer, pH 7.0, 1 mM dithiothreitol, at least 1 month
Equus caballus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
17alpha-estradiol + NAD(P)+
-
285970
Equus caballus
estrone + NAD(P)H
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 33000, SDS-PAGE
Equus caballus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
9.5
-
Equus caballus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
8
10
-
Equus caballus
Other publictions for EC 1.1.1.148
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
668078
Bellemare
Characterization of 17alpha-hy ...
Mus musculus
BMC Biochem.
6
12
2005
-
-
1
-
-
-
-
-
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1
-
4
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1
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2
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8
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1
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1
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1
1
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1
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1
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2
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8
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1
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-
285976
De Prada
Purification and characterizat ...
Eubacterium sp., Eubacterium sp. VPI 12708
J. Lipid Res.
35
922-929
1994
-
-
-
-
-
1
1
4
-
2
2
-
-
12
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1
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-
2
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4
1
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2
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1
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1
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1
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1
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4
-
2
2
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1
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2
-
4
1
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-
2
-
-
-
-
-
-
-
-
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285970
LaRhee
Purification and characterizat ...
Equus caballus
Biochemistry
23
486-491
1984
-
-
-
-
-
-
-
1
-
-
2
-
-
2
-
-
1
-
-
2
-
1
1
1
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1
1
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2
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2
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1
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2
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1
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2
-
1
1
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
285971
Johnston
Oestradiol-17alpha dehydrogena ...
Gallus gallus
Biochem. J.
222
761-768
1984
-
-
-
-
-
-
1
2
-
-
-
-
-
4
-
-
1
-
-
2
1
1
4
-
-
-
-
-
1
-
-
2
-
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-
-
-
-
2
-
-
-
-
1
-
2
-
-
-
-
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1
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2
1
1
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
285972
Hasnain
Properties of the multiple for ...
Oryctolagus cuniculus
Biochem. J.
161
279-283
1977
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-
-
-
-
-
1
3
1
-
1
-
-
2
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1
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-
2
5
-
5
1
-
-
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2
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-
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-
-
2
-
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1
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3
1
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1
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1
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2
5
-
5
1
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285973
Hasnain
Purification of multiple forms ...
Oryctolagus cuniculus
Biochem. J.
147
457-461
1975
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2
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1
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2
1
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3
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1
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1
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3
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285975
Hasnain
The separation and partial pur ...
Oryctolagus cuniculus
Can. J. Biochem.
52
120-125
1974
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-
-
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1
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1
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1
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2
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2
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2
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1
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1
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2
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285974
Renwick
The partial purification of 17 ...
Gallus gallus
Biochim. Biophys. Acta
146
336-348
1967
-
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-
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1
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1
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1
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1
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1
1
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1
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3
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3
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1
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1
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1
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1
1
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1
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