BRENDA - Enzyme Database show
show all sequences of 1.1.1.147

16alpha-Hydroxysteroid dehydrogenase of rat kidney. Purification, assay, and properties

Meigs, R.A.; Ryan, K.J.; J. Biol. Chem. 241, 4011-4015 (1966)

Data extracted from this reference:

General Stability
General Stability
Organism
extremely unstable, glycerol partially protects against inactivation
Rattus sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
complete inhibition at 0.1 mM
Rattus sp.
2,2'-bipyridine
19% inhibition at 0.1 mM
Rattus sp.
2-Iodoacetamide
86% inhibition at 1 mM
Rattus sp.
8-hydroxy-5-quinolinesulfonate
89% inhibition at 1 mM
Rattus sp.
Ag+
78% inhibition at 0.01 mM
Rattus sp.
Cu2+
98% inhibition at 0.01 mM
Rattus sp.
EDTA
97% inhibition at 0.01 mM
Rattus sp.
N-ethylmaleimide
94% inhibition at 0.1 mM
Rattus sp.
o-Iodosobenzoate
complete inhibition at 0.1 mM
Rattus sp.
p-hydroxymercuribenzoate
complete inhibition at 0.01 mM
Rattus sp.
Zn2+
84% inhibition at 0.1 mM
Rattus sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.041
-
NADPH
-
Rattus sp.
0.07
-
17alpha-estradiol-16-one
-
Rattus sp.
0.083
-
NADH
-
Rattus sp.
0.089
-
17beta-estradiol-16-one
-
Rattus sp.
0.28
-
16-ketotestosterone
-
Rattus sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus sp.
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Rattus sp.
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
inducible by estrogen administration
Rattus sp.
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
16-ketotestosterone + NAD(P)H
-
285968
Rattus sp.
16alpha-hydroxytestosterone + NAD(P)+
-
285968
Rattus sp.
r
17alpha-estradiol-16-one + NAD(P)H
best substrate tested, changing configuration or oxidation state of the neighboring oxygen substituent at C-17 increases the rate of reduction
285968
Rattus sp.
16alpha-hydroxy-17alpha-estradiol + NAD(P)+
-
285968
Rattus sp.
r
17beta-estradiol-16-one + NAD(P)H
-
285968
Rattus sp.
16alpha-hydroxy-17beta-estradiol + NAD(P)+
-
285968
Rattus sp.
r
3-hydroxy-estra-1,3,5(10)-triene-16,17-dione + NAD(P)H
-
285968
Rattus sp.
3,16alpha-dihydroxy-estra-1,3,5(10)-triene-17-one + NAD(P)H
-
285968
Rattus sp.
r
3beta,17beta-dihydroxy-androst-5-en-16-one + NAD(P)H
low activity
285968
Rattus sp.
3beta,16alpha,17beta-trihydroxyandrost-5-ene + NAD(P)+
-
285968
Rattus sp.
?
estra-1,3,5(10)-triene-3,16beta,17beta-triol + NAD(P)+
-
285968
Rattus sp.
estra-1,3,5(10)-triene-3,17beta-diol-16-one + NAD(P)H
-
285968
Rattus sp.
?
estra-1,3,5(10)triene-3,17-beta-diol-16-one + NAD(P)H
low activity
285968
Rattus sp.
estra-1,3,5(10)triene-3,16alpha,17-triol + NAD(P)+
-
285968
Rattus sp.
r
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.1
6.2
-
Rattus sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
40% lower reaction rate than NADPH
Rattus sp.
NADPH
40% greater reaction rate than NADH
Rattus sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
40% lower reaction rate than NADPH
Rattus sp.
NADPH
40% greater reaction rate than NADH
Rattus sp.
General Stability (protein specific)
General Stability
Organism
extremely unstable, glycerol partially protects against inactivation
Rattus sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
complete inhibition at 0.1 mM
Rattus sp.
2,2'-bipyridine
19% inhibition at 0.1 mM
Rattus sp.
2-Iodoacetamide
86% inhibition at 1 mM
Rattus sp.
8-hydroxy-5-quinolinesulfonate
89% inhibition at 1 mM
Rattus sp.
Ag+
78% inhibition at 0.01 mM
Rattus sp.
Cu2+
98% inhibition at 0.01 mM
Rattus sp.
EDTA
97% inhibition at 0.01 mM
Rattus sp.
N-ethylmaleimide
94% inhibition at 0.1 mM
Rattus sp.
o-Iodosobenzoate
complete inhibition at 0.1 mM
Rattus sp.
p-hydroxymercuribenzoate
complete inhibition at 0.01 mM
Rattus sp.
Zn2+
84% inhibition at 0.1 mM
Rattus sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.041
-
NADPH
-
Rattus sp.
0.07
-
17alpha-estradiol-16-one
-
Rattus sp.
0.083
-
NADH
-
Rattus sp.
0.089
-
17beta-estradiol-16-one
-
Rattus sp.
0.28
-
16-ketotestosterone
-
Rattus sp.
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Rattus sp.
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
inducible by estrogen administration
Rattus sp.
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
16-ketotestosterone + NAD(P)H
-
285968
Rattus sp.
16alpha-hydroxytestosterone + NAD(P)+
-
285968
Rattus sp.
r
17alpha-estradiol-16-one + NAD(P)H
best substrate tested, changing configuration or oxidation state of the neighboring oxygen substituent at C-17 increases the rate of reduction
285968
Rattus sp.
16alpha-hydroxy-17alpha-estradiol + NAD(P)+
-
285968
Rattus sp.
r
17beta-estradiol-16-one + NAD(P)H
-
285968
Rattus sp.
16alpha-hydroxy-17beta-estradiol + NAD(P)+
-
285968
Rattus sp.
r
3-hydroxy-estra-1,3,5(10)-triene-16,17-dione + NAD(P)H
-
285968
Rattus sp.
3,16alpha-dihydroxy-estra-1,3,5(10)-triene-17-one + NAD(P)H
-
285968
Rattus sp.
r
3beta,17beta-dihydroxy-androst-5-en-16-one + NAD(P)H
low activity
285968
Rattus sp.
3beta,16alpha,17beta-trihydroxyandrost-5-ene + NAD(P)+
-
285968
Rattus sp.
?
estra-1,3,5(10)-triene-3,16beta,17beta-triol + NAD(P)+
-
285968
Rattus sp.
estra-1,3,5(10)-triene-3,17beta-diol-16-one + NAD(P)H
-
285968
Rattus sp.
?
estra-1,3,5(10)triene-3,17-beta-diol-16-one + NAD(P)H
low activity
285968
Rattus sp.
estra-1,3,5(10)triene-3,16alpha,17-triol + NAD(P)+
-
285968
Rattus sp.
r
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.1
6.2
-
Rattus sp.
Other publictions for EC 1.1.1.147
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
285969
Preumont
16-Dehydrogenase in human plac ...
Homo sapiens
Acta Endocrinol.
78
760-765
1975
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285968
Meigs
16alpha-Hydroxysteroid dehydro ...
Rattus sp.
J. Biol. Chem.
241
4011-4015
1966
-
-
-
-
-
1
11
5
-
-
-
-
-
1
-
-
1
-
-
1
-
-
7
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
1
-
11
-
5
-
-
-
-
-
-
-
1
-
1
-
-
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-