BRENDA - Enzyme Database show
show all sequences of 1.1.1.145

The functions of key residues in the inhibitor, substrate and cofactor sites of human 3beta-hydroxysteroid dehydrogenase type 1 are validated by mutagenesis

Thomas, J.L.; Mack, V.L.; Sun, J.; Terrell, J.R.; Bucholtz, K.M.; J. Steroid Biochem. Mol. Biol. 120, 192-199 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Sf9 cells using the baculovirus system; expressed in Sf9 cells using the baculovirus system
Homo sapiens
Engineering
Amino acid exchange
Commentary
Organism
D61N
D61N mutation produces a 1.3fold increase in Km for the dehydroepiandrosterone substrate, a 1.9fold increase in Km for NAD+ utilization by the 3beta-HSD activity and little change in isomerase kinetics for either substrate or cofactor
Homo sapiens
D61V
mutant shows no enzymatic activity
Homo sapiens
E192A
the E192A mutation causes an 8.2fold increase in Km (dehydroepiandrosterone) and no change in the Km for NAD+
Homo sapiens
R195P
R195P mutation shifts the high-affinity, competitive inhibition profile of 3beta-HSD1 to a low-affinity (Ki= 2.56 microM), noncompetitive inhibition profile. R195P mutant has a 10.4fold higher Km value for 16beta-hydroxy-dehydroepiandrosterone compared to wild-type 3beta-HSD1. Km values for 16alpha-hydroxy-dehydroepiandrosterone and dehydroepiandrosterone are also inreased compared to wild-type (kcat values are decreased compared to wild-type)
Homo sapiens
R195R
the P195R mutation shifts the low-affinity, noncompetitive inhibition profile of 3beta-HSD2 to a high affinity (Ki= 0.19 microM), competitive inhibition profile. The P195R mutant has a 12.2fold lower Km value for 16beta-hydroxy-dehydroepiandrosterone compared to wild-type 3beta-HSD2. The coenzyme values measured for P195R mutant are similar to those measured for wildtype 3beta-HSD2
Homo sapiens
T8A
mutant shows almost similar kinetic values as wild-type
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
trilostane
;
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0014
-
16beta-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD1, pH 7.4, 27°C
Homo sapiens
0.0015
-
16beta-hydroxy-dehydroepiandrosterone
mutant R195R, pH 7.4, 27°C
Homo sapiens
0.0025
-
NADH
mutant E192A, pH 7.4, 27°C
Homo sapiens
0.0036
-
NADH
mutant R195P, pH 7.4, 27°C
Homo sapiens
0.0037
-
dehydroepiandrosterone
wild-type 3beta-HSD1, pH 7.4, 27°C
Homo sapiens
0.004
-
16alpha-hydroxy-dehydroepiandrosterone
mutant R195R, pH 7.4, 27°C
Homo sapiens
0.0046
-
NADH
wild-type 3beta-HSD1, pH 7.4, 27°C
Homo sapiens
0.0055
-
NADH
mutant R195R, pH 7.4, 27°C
Homo sapiens
0.0064
-
16alpha-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD1, pH 7.4, 27°C
Homo sapiens
0.0065
-
NADH
mutant E192A, pH 7.4, 27°C
Homo sapiens
0.0072
-
NADH
mutant D61N, pH 7.4, 27°C
Homo sapiens
0.0077
-
dehydroepiandrosterone
mutant R195R, pH 7.4, 27°C
Homo sapiens
0.0082
-
dehydroepiandrosterone
mutant E192A, pH 7.4, 27°C
Homo sapiens
0.0107
-
dehydroepiandrosterone
mutant R195P, pH 7.4, 27°C
Homo sapiens
0.0126
-
NADH
wild-type 3beta-HSD2, pH 7.4, 27°C
Homo sapiens
0.0148
-
16alpha-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD2, pH 7.4, 27°C
Homo sapiens
0.015
-
16beta-hydroxy-dehydroepiandrosterone
mutant R195P, pH 7.4, 27°C
Homo sapiens
0.0156
-
dehydroepiandrosterone
wild-type 3beta-HSD2, pH 7.4, 27°C
Homo sapiens
0.0184
-
16beta-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD2, pH 7.4, 27°C
Homo sapiens
0.0196
-
16alpha-hydroxy-dehydroepiandrosterone
mutant R195P, pH 7.4, 27°C
Homo sapiens
0.0205
-
NAD+
mutant R195P, pH 7.4, 27°C
Homo sapiens
0.0333
-
NAD+
mutant E192A, pH 7.4, 27°C
Homo sapiens
0.0341
-
NAD+
wild-type 3beta-HSD1, pH 7.4, 27°C
Homo sapiens
0.0351
-
dehydroepiandrosterone
mutant E192A, pH 7.4, 27°C
Homo sapiens
0.0351
-
NAD+
mutant E192A, pH 7.4, 27°C
Homo sapiens
0.0564
-
NAD+
mutant R195R, pH 7.4, 27°C
Homo sapiens
0.0643
-
dehydroepiandrosterone
mutant D61N, pH 7.4, 27°C
Homo sapiens
0.0643
-
NAD+
mutant D61N, pH 7.4, 27°C
Homo sapiens
0.0863
-
NAD+
wild-type 3beta-HSD2, pH 7.4, 27°C
Homo sapiens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
SDS-PAGE; SDS-PAGE
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
P14060
-
-
Homo sapiens
P26439
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
16alpha-hydroxy-dehydroepiandrosterone + NAD+
-
725813
Homo sapiens
?
-
-
-
?
16alpha-hydroxy-dehydroepiandrosterone + NAD+
-
725813
Homo sapiens
? + NADH
-
-
-
?
16beta-hydroxy-dehydroepiandrosterone + NAD+
-
725813
Homo sapiens
?
-
-
-
?
16beta-hydroxy-dehydroepiandrosterone + NAD+
-
725813
Homo sapiens
? + NADH
-
-
-
?
androst-5-en-3,17-dione + NADH
-
725813
Homo sapiens
dehydroepiandrosterone + NAD+
-
-
-
r
dehydroepiandrosterone + NAD+
-
725813
Homo sapiens
androst-5-en-3,17-dione + NADH
-
-
-
?
dehydroepiandrosterone + NAD+
-
725813
Homo sapiens
androst-5-en-3,17-dione + NADH
-
-
-
r
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
27
-
assay at; assay at
Homo sapiens
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.02
-
NAD+
mutant E192A, pH 7.4, 27°C
Homo sapiens
0.03
-
dehydroepiandrosterone
mutant E192A, pH 7.4, 27°C
Homo sapiens
0.04
-
16beta-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD1, pH 7.4, 27°C
Homo sapiens
0.04
-
dehydroepiandrosterone
mutant D61N, pH 7.4, 27°C
Homo sapiens
0.04
-
NAD+
mutant E192A, pH 7.4, 27°C
Homo sapiens
0.05
-
16beta-hydroxy-dehydroepiandrosterone
mutant R195R, pH 7.4, 27°C
Homo sapiens
0.05
-
dehydroepiandrosterone
wild-type 3beta-HSD1, pH 7.4, 27°C
Homo sapiens
0.05
-
NAD+
wild-type 3beta-HSD1, pH 7.4, 27°C
Homo sapiens
0.06
-
dehydroepiandrosterone
mutant E192A, pH 7.4, 27°C
Homo sapiens
0.06
-
NAD+
mutant D61N, pH 7.4, 27°C
Homo sapiens
0.07
-
16alpha-hydroxy-dehydroepiandrosterone
mutant R195R, pH 7.4, 27°C
Homo sapiens
0.09
-
16alpha-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD1, pH 7.4, 27°C
Homo sapiens
0.09
-
dehydroepiandrosterone
mutant R195R, pH 7.4, 27°C
Homo sapiens
0.1
-
NAD+
mutant R195P, pH 7.4, 27°C
Homo sapiens
0.11
-
NAD+
wild-type 3beta-HSD2, pH 7.4, 27°C
Homo sapiens
0.12
-
dehydroepiandrosterone
mutant R195P, pH 7.4, 27°C
Homo sapiens
0.14
-
16beta-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD2, pH 7.4, 27°C
Homo sapiens
0.14
-
dehydroepiandrosterone
wild-type 3beta-HSD2, pH 7.4, 27°C
Homo sapiens
0.15
-
16alpha-hydroxy-dehydroepiandrosterone
mutant R195P, pH 7.4, 27°C; wild-type 3beta-HSD2, pH 7.4, 27°C
Homo sapiens
0.15
-
16beta-hydroxy-dehydroepiandrosterone
mutant R195P, pH 7.4, 27°C
Homo sapiens
0.17
-
NAD+
mutant R195R, pH 7.4, 27°C
Homo sapiens
0.31
-
NADH
mutant E192A, pH 7.4, 27°C
Homo sapiens
0.69
-
NADH
mutant E192A, pH 7.4, 27°C
Homo sapiens
0.75
-
NADH
wild-type 3beta-HSD1, pH 7.4, 27°C
Homo sapiens
0.76
-
NADH
mutant D61N, pH 7.4, 27°C
Homo sapiens
1.03
-
NADH
mutant R195P, pH 7.4, 27°C
Homo sapiens
1.49
-
NADH
mutant R195R, pH 7.4, 27°C
Homo sapiens
1.65
-
NADH
wild-type 3beta-HSD2, pH 7.4, 27°C
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at; assay at
Homo sapiens
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.00001
-
trilostane
pH 7.4, 27°C, wild-tpye 3beta-HSD1
Homo sapiens
0.00002
-
trilostane
pH 7.4, 27°C, mutant R195R
Homo sapiens
0.0001
-
trilostane
pH 7.4, 27°C, wild-tpye 3beta-HSD2
Homo sapiens
0.00022
-
trilostane
pH 7.4, 27°C, mutant R195P
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Sf9 cells using the baculovirus system
Homo sapiens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D61N
D61N mutation produces a 1.3fold increase in Km for the dehydroepiandrosterone substrate, a 1.9fold increase in Km for NAD+ utilization by the 3beta-HSD activity and little change in isomerase kinetics for either substrate or cofactor
Homo sapiens
D61V
mutant shows no enzymatic activity
Homo sapiens
E192A
the E192A mutation causes an 8.2fold increase in Km (dehydroepiandrosterone) and no change in the Km for NAD+
Homo sapiens
R195P
R195P mutation shifts the high-affinity, competitive inhibition profile of 3beta-HSD1 to a low-affinity (Ki= 2.56 microM), noncompetitive inhibition profile. R195P mutant has a 10.4fold higher Km value for 16beta-hydroxy-dehydroepiandrosterone compared to wild-type 3beta-HSD1. Km values for 16alpha-hydroxy-dehydroepiandrosterone and dehydroepiandrosterone are also inreased compared to wild-type (kcat values are decreased compared to wild-type)
Homo sapiens
R195R
the P195R mutation shifts the low-affinity, noncompetitive inhibition profile of 3beta-HSD2 to a high affinity (Ki= 0.19 microM), competitive inhibition profile. The P195R mutant has a 12.2fold lower Km value for 16beta-hydroxy-dehydroepiandrosterone compared to wild-type 3beta-HSD2. The coenzyme values measured for P195R mutant are similar to those measured for wildtype 3beta-HSD2
Homo sapiens
T8A
mutant shows almost similar kinetic values as wild-type
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
trilostane
-
Homo sapiens
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.00001
-
trilostane
pH 7.4, 27°C, wild-tpye 3beta-HSD1
Homo sapiens
0.00002
-
trilostane
pH 7.4, 27°C, mutant R195R
Homo sapiens
0.0001
-
trilostane
pH 7.4, 27°C, wild-tpye 3beta-HSD2
Homo sapiens
0.00022
-
trilostane
pH 7.4, 27°C, mutant R195P
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0014
-
16beta-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD1, pH 7.4, 27°C
Homo sapiens
0.0015
-
16beta-hydroxy-dehydroepiandrosterone
mutant R195R, pH 7.4, 27°C
Homo sapiens
0.0025
-
NADH
mutant E192A, pH 7.4, 27°C
Homo sapiens
0.0036
-
NADH
mutant R195P, pH 7.4, 27°C
Homo sapiens
0.0037
-
dehydroepiandrosterone
wild-type 3beta-HSD1, pH 7.4, 27°C
Homo sapiens
0.004
-
16alpha-hydroxy-dehydroepiandrosterone
mutant R195R, pH 7.4, 27°C
Homo sapiens
0.0046
-
NADH
wild-type 3beta-HSD1, pH 7.4, 27°C
Homo sapiens
0.0055
-
NADH
mutant R195R, pH 7.4, 27°C
Homo sapiens
0.0064
-
16alpha-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD1, pH 7.4, 27°C
Homo sapiens
0.0065
-
NADH
mutant E192A, pH 7.4, 27°C
Homo sapiens
0.0072
-
NADH
mutant D61N, pH 7.4, 27°C
Homo sapiens
0.0077
-
dehydroepiandrosterone
mutant R195R, pH 7.4, 27°C
Homo sapiens
0.0082
-
dehydroepiandrosterone
mutant E192A, pH 7.4, 27°C
Homo sapiens
0.0107
-
dehydroepiandrosterone
mutant R195P, pH 7.4, 27°C
Homo sapiens
0.0126
-
NADH
wild-type 3beta-HSD2, pH 7.4, 27°C
Homo sapiens
0.0148
-
16alpha-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD2, pH 7.4, 27°C
Homo sapiens
0.015
-
16beta-hydroxy-dehydroepiandrosterone
mutant R195P, pH 7.4, 27°C
Homo sapiens
0.0156
-
dehydroepiandrosterone
wild-type 3beta-HSD2, pH 7.4, 27°C
Homo sapiens
0.0184
-
16beta-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD2, pH 7.4, 27°C
Homo sapiens
0.0196
-
16alpha-hydroxy-dehydroepiandrosterone
mutant R195P, pH 7.4, 27°C
Homo sapiens
0.0205
-
NAD+
mutant R195P, pH 7.4, 27°C
Homo sapiens
0.0333
-
NAD+
mutant E192A, pH 7.4, 27°C
Homo sapiens
0.0341
-
NAD+
wild-type 3beta-HSD1, pH 7.4, 27°C
Homo sapiens
0.0351
-
dehydroepiandrosterone
mutant E192A, pH 7.4, 27°C
Homo sapiens
0.0351
-
NAD+
mutant E192A, pH 7.4, 27°C
Homo sapiens
0.0564
-
NAD+
mutant R195R, pH 7.4, 27°C
Homo sapiens
0.0643
-
dehydroepiandrosterone
mutant D61N, pH 7.4, 27°C
Homo sapiens
0.0643
-
NAD+
mutant D61N, pH 7.4, 27°C
Homo sapiens
0.0863
-
NAD+
wild-type 3beta-HSD2, pH 7.4, 27°C
Homo sapiens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
SDS-PAGE
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
16alpha-hydroxy-dehydroepiandrosterone + NAD+
-
725813
Homo sapiens
?
-
-
-
?
16alpha-hydroxy-dehydroepiandrosterone + NAD+
-
725813
Homo sapiens
? + NADH
-
-
-
?
16beta-hydroxy-dehydroepiandrosterone + NAD+
-
725813
Homo sapiens
?
-
-
-
?
16beta-hydroxy-dehydroepiandrosterone + NAD+
-
725813
Homo sapiens
? + NADH
-
-
-
?
androst-5-en-3,17-dione + NADH
-
725813
Homo sapiens
dehydroepiandrosterone + NAD+
-
-
-
r
dehydroepiandrosterone + NAD+
-
725813
Homo sapiens
androst-5-en-3,17-dione + NADH
-
-
-
?
dehydroepiandrosterone + NAD+
-
725813
Homo sapiens
androst-5-en-3,17-dione + NADH
-
-
-
r
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
27
-
assay at
Homo sapiens
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.02
-
NAD+
mutant E192A, pH 7.4, 27°C
Homo sapiens
0.03
-
dehydroepiandrosterone
mutant E192A, pH 7.4, 27°C
Homo sapiens
0.04
-
16beta-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD1, pH 7.4, 27°C
Homo sapiens
0.04
-
dehydroepiandrosterone
mutant D61N, pH 7.4, 27°C
Homo sapiens
0.04
-
NAD+
mutant E192A, pH 7.4, 27°C
Homo sapiens
0.05
-
16beta-hydroxy-dehydroepiandrosterone
mutant R195R, pH 7.4, 27°C
Homo sapiens
0.05
-
dehydroepiandrosterone
wild-type 3beta-HSD1, pH 7.4, 27°C
Homo sapiens
0.05
-
NAD+
wild-type 3beta-HSD1, pH 7.4, 27°C
Homo sapiens
0.06
-
dehydroepiandrosterone
mutant E192A, pH 7.4, 27°C
Homo sapiens
0.06
-
NAD+
mutant D61N, pH 7.4, 27°C
Homo sapiens
0.07
-
16alpha-hydroxy-dehydroepiandrosterone
mutant R195R, pH 7.4, 27°C
Homo sapiens
0.09
-
16alpha-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD1, pH 7.4, 27°C
Homo sapiens
0.09
-
dehydroepiandrosterone
mutant R195R, pH 7.4, 27°C
Homo sapiens
0.1
-
NAD+
mutant R195P, pH 7.4, 27°C
Homo sapiens
0.11
-
NAD+
wild-type 3beta-HSD2, pH 7.4, 27°C
Homo sapiens
0.12
-
dehydroepiandrosterone
mutant R195P, pH 7.4, 27°C
Homo sapiens
0.14
-
16beta-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD2, pH 7.4, 27°C
Homo sapiens
0.14
-
dehydroepiandrosterone
wild-type 3beta-HSD2, pH 7.4, 27°C
Homo sapiens
0.15
-
16alpha-hydroxy-dehydroepiandrosterone
mutant R195P, pH 7.4, 27°C
Homo sapiens
0.15
-
16alpha-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD2, pH 7.4, 27°C
Homo sapiens
0.15
-
16beta-hydroxy-dehydroepiandrosterone
mutant R195P, pH 7.4, 27°C
Homo sapiens
0.17
-
NAD+
mutant R195R, pH 7.4, 27°C
Homo sapiens
0.31
-
NADH
mutant E192A, pH 7.4, 27°C
Homo sapiens
0.69
-
NADH
mutant E192A, pH 7.4, 27°C
Homo sapiens
0.75
-
NADH
wild-type 3beta-HSD1, pH 7.4, 27°C
Homo sapiens
0.76
-
NADH
mutant D61N, pH 7.4, 27°C
Homo sapiens
1.03
-
NADH
mutant R195P, pH 7.4, 27°C
Homo sapiens
1.49
-
NADH
mutant R195R, pH 7.4, 27°C
Homo sapiens
1.65
-
NADH
wild-type 3beta-HSD2, pH 7.4, 27°C
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Homo sapiens
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.83
-
NAD+
mutant E192A, pH 7.4, 27°C
Homo sapiens
1
-
dehydroepiandrosterone
mutant E192A, pH 7.4, 27°C
Homo sapiens
1
-
NAD+
mutant D61N, pH 7.4, 27°C
Homo sapiens
1.33
-
NAD+
mutant E192A, pH 7.4, 27°C; wild-type 3beta-HSD2, pH 7.4, 27°C
Homo sapiens
1.66
-
NAD+
wild-type 3beta-HSD1, pH 7.4, 27°C
Homo sapiens
3.16
-
NAD+
mutant R195R, pH 7.4, 27°C
Homo sapiens
4.83
-
NAD+
mutant R195P, pH 7.4, 27°C
Homo sapiens
7.33
-
dehydroepiandrosterone
mutant E192A, pH 7.4, 27°C; wild-type 3beta-HSD2, pH 7.4, 27°C
Homo sapiens
7.66
-
16beta-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD2, pH 7.4, 27°C
Homo sapiens
7.83
-
16alpha-hydroxy-dehydroepiandrosterone
mutant R195P, pH 7.4, 27°C
Homo sapiens
10
-
dehydroepiandrosterone
mutant D61N, pH 7.4, 27°C
Homo sapiens
10.16
-
16alpha-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD2, pH 7.4, 27°C
Homo sapiens
10.33
-
16beta-hydroxy-dehydroepiandrosterone
mutant R195P, pH 7.4, 27°C
Homo sapiens
11.21
-
dehydroepiandrosterone
mutant R195P, pH 7.4, 27°C
Homo sapiens
12.16
-
dehydroepiandrosterone
mutant R195R, pH 7.4, 27°C
Homo sapiens
14.83
-
dehydroepiandrosterone
wild-type 3beta-HSD1, pH 7.4, 27°C
Homo sapiens
15
-
16alpha-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD1, pH 7.4, 27°C
Homo sapiens
19.1
-
16alpha-hydroxy-dehydroepiandrosterone
mutant R195R, pH 7.4, 27°C
Homo sapiens
34.5
-
16beta-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD1, pH 7.4, 27°C
Homo sapiens
35.5
-
16beta-hydroxy-dehydroepiandrosterone
mutant R195R, pH 7.4, 27°C
Homo sapiens
106
-
NADH
mutant D61N, pH 7.4, 27°C; mutant E192A, pH 7.4, 27°C
Homo sapiens
125
-
NADH
mutant E192A, pH 7.4, 27°C
Homo sapiens
131
-
NADH
wild-type 3beta-HSD2, pH 7.4, 27°C
Homo sapiens
163
-
NADH
wild-type 3beta-HSD1, pH 7.4, 27°C
Homo sapiens
271
-
NADH
mutant R195R, pH 7.4, 27°C
Homo sapiens
280
-
NADH
mutant R195P, pH 7.4, 27°C
Homo sapiens
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.83
-
NAD+
mutant E192A, pH 7.4, 27°C
Homo sapiens
1
-
dehydroepiandrosterone
mutant E192A, pH 7.4, 27°C
Homo sapiens
1
-
NAD+
mutant D61N, pH 7.4, 27°C
Homo sapiens
1.33
-
NAD+
mutant E192A, pH 7.4, 27°C
Homo sapiens
1.33
-
NAD+
wild-type 3beta-HSD2, pH 7.4, 27°C
Homo sapiens
1.66
-
NAD+
wild-type 3beta-HSD1, pH 7.4, 27°C
Homo sapiens
3.16
-
NAD+
mutant R195R, pH 7.4, 27°C
Homo sapiens
4.83
-
NAD+
mutant R195P, pH 7.4, 27°C
Homo sapiens
7.33
-
dehydroepiandrosterone
mutant E192A, pH 7.4, 27°C
Homo sapiens
7.33
-
dehydroepiandrosterone
wild-type 3beta-HSD2, pH 7.4, 27°C
Homo sapiens
7.66
-
16beta-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD2, pH 7.4, 27°C
Homo sapiens
7.83
-
16alpha-hydroxy-dehydroepiandrosterone
mutant R195P, pH 7.4, 27°C
Homo sapiens
10
-
dehydroepiandrosterone
mutant D61N, pH 7.4, 27°C
Homo sapiens
10.16
-
16alpha-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD2, pH 7.4, 27°C
Homo sapiens
10.33
-
16beta-hydroxy-dehydroepiandrosterone
mutant R195P, pH 7.4, 27°C
Homo sapiens
11.21
-
dehydroepiandrosterone
mutant R195P, pH 7.4, 27°C
Homo sapiens
12.16
-
dehydroepiandrosterone
mutant R195R, pH 7.4, 27°C
Homo sapiens
14.83
-
dehydroepiandrosterone
wild-type 3beta-HSD1, pH 7.4, 27°C
Homo sapiens
15
-
16alpha-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD1, pH 7.4, 27°C
Homo sapiens
19.1
-
16alpha-hydroxy-dehydroepiandrosterone
mutant R195R, pH 7.4, 27°C
Homo sapiens
34.5
-
16beta-hydroxy-dehydroepiandrosterone
wild-type 3beta-HSD1, pH 7.4, 27°C
Homo sapiens
35.5
-
16beta-hydroxy-dehydroepiandrosterone
mutant R195R, pH 7.4, 27°C
Homo sapiens
106
-
NADH
mutant D61N, pH 7.4, 27°C; mutant E192A, pH 7.4, 27°C
Homo sapiens
125
-
NADH
mutant E192A, pH 7.4, 27°C
Homo sapiens
131
-
NADH
wild-type 3beta-HSD2, pH 7.4, 27°C
Homo sapiens
163
-
NADH
wild-type 3beta-HSD1, pH 7.4, 27°C
Homo sapiens
271
-
NADH
mutant R195R, pH 7.4, 27°C
Homo sapiens
280
-
NADH
mutant R195P, pH 7.4, 27°C
Homo sapiens
Other publictions for EC 1.1.1.145
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725807
Wojciechowicz
The activity and localization ...
Sus scrofa
J. Reprod. Dev.
59
49-58
2013
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2
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2
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-
2
-
-
725511
Prasad
Mitochondrial 3beta-hydroxyste ...
Homo sapiens
J. Biol. Chem.
287
9534-9546
2012
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-
1
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1
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1
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1
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1
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1
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2
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1
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1
1
1
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1
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1
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1
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1
1
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-
-
2
-
1
-
-
-
1
1
1
-
-
-
-
-
-
-
725931
Rasmussen
Expression of hepatic 3beta-hy ...
Sus scrofa
Mol. Biol. Rep.
39
7927-32
2012
-
1
-
-
-
-
-
-
-
-
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1
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1
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-
-
-
-
-
-
-
1
-
-
1
-
-
724302
Rajapaksha
Lipid-mediated unfolding of 3b ...
Homo sapiens
Biochemistry
50
11015-11024
2011
-
-
1
-
-
-
1
-
1
-
1
1
-
1
-
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1
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-
-
3
-
1
-
3
-
-
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1
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1
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1
-
1
1
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-
1
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-
-
3
-
1
-
3
-
-
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-
-
-
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-
-
-
-
724518
Thomas
Inhibition of the M. tuberculo ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Bioorg. Med. Chem. Lett.
21
2216-2219
2011
-
-
-
-
-
-
20
-
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2
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2
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1
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1
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20
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20
20
-
-
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-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
725447
Pawlak
Inner mitochondrial translocas ...
Sus scrofa
J. Biol. Chem.
286
39130-39140
2011
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-
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1
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1
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1
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1
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1
-
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-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
725612
Zhao
Homology modeling and molecula ...
Homo sapiens
J. Comput. Chem.
32
33-42
2011
-
-
-
-
1
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-
-
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3
-
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-
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2
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-
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2
-
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
725816
Nakamura
Human adrenal cells that expre ...
Homo sapiens
J. Steroid Biochem. Mol. Biol.
123
122-126
2011
-
-
-
-
-
-
1
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-
-
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2
-
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1
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1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
725820
Thomas
Selective inhibition of human ...
Homo sapiens
J. Steroid Biochem. Mol. Biol.
125
57-65
2011
-
-
1
-
3
-
2
7
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
7
-
-
-
-
9
-
-
-
-
2
-
-
5
-
-
4
9
7
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
7
-
-
-
-
-
-
-
-
5
6
713188
Nittono
3?-hydroxy-??-C??-steroid dehy ...
Homo sapiens
Pediatr. Internat.
52
e192-e195
2010
-
1
-
-
-
-
-
-
-
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1
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1
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-
-
-
713329
Ernst
Expression of 3beta-HSD and P5 ...
Digitalis lanata, Digitalis lanata EHRH
Planta Med.
76
923-927
2010
-
-
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2
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2
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4
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2
-
-
4
-
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-
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-
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-
-
-
725813
Thomas
The functions of key residues ...
Homo sapiens
J. Steroid Biochem. Mol. Biol.
120
192-199
2010
-
-
1
-
6
-
1
29
-
-
1
-
-
2
-
-
-
-
-
-
-
-
7
-
1
-
-
28
1
-
-
-
4
-
-
-
-
2
-
-
6
-
-
2
4
29
-
-
2
-
-
-
-
-
-
-
-
-
7
-
2
-
-
29
2
-
-
-
-
-
-
-
26
28
698503
Lysiak
Hypoxia-inducible factor-1alph ...
Mus musculus
J. Androl.
30
146-156
2009
1
-
-
-
-
-
-
-
-
-
-
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-
1
-
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-
-
3
-
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-
1
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-
-
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-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
699845
Hu
The (+)- and (-)-gossypols pot ...
Homo sapiens, Rattus norvegicus
J. Steroid Biochem. Mol. Biol.
115
14-19
2009
-
-
-
-
-
-
4
-
2
-
-
-
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2
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-
-
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2
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2
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2
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4
-
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2
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4
4
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2
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-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
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-
-
700182
Thomas
Structural basis for the selec ...
Homo sapiens
Mol. Cell. Endocrinol.
301
174-182
2009
-
-
-
-
-
-
3
2
-
-
1
-
-
2
-
-
-
-
-
3
-
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1
-
-
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-
2
-
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1
2
-
2
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-
-
1
-
-
-
2
3
2
2
-
-
1
-
-
-
-
-
-
3
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
684725
Matsunaga
Characterization of human DHRS ...
Homo sapiens
Arch. Biochem. Biophys.
477
339-347
2008
-
-
-
-
1
-
6
37
1
-
-
-
-
3
-
-
-
-
-
1
-
-
43
-
-
-
-
35
2
-
-
3
-
-
4
-
-
-
3
-
1
-
4
6
-
37
1
-
-
-
-
-
-
-
-
1
-
-
43
-
-
-
-
35
2
-
-
-
-
-
-
-
-
-
687967
Welzel
Carboxyl-terminal mutations in ...
Homo sapiens
J. Clin. Endocrinol. Metab.
93
1418-1425
2008
-
1
-
-
3
-
-
-
-
-
-
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1
-
-
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-
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1
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3
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
693887
Thomas
Structure/function of the inhi ...
Homo sapiens
J. Steroid Biochem. Mol. Biol.
111
66-73
2008
-
-
1
-
2
-
2
-
-
-
1
-
-
1
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
1
8
-
-
-
-
1
1
-
2
-
-
2
8
-
-
-
1
-
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
699111
Parthasarathy
Effects of corticosterone defi ...
Rattus norvegicus
J. Cell. Biochem.
104
1671-1683
2008
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
700557
Fernandez Presas
The key steroidogenic enzyme 3 ...
Taenia crassiceps, Taenia crassiceps WFU, Taenia solium
Parasitol. Res.
103
847-852
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
9
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
681643
Meffre
3beta-Hydroxysteroid dehydroge ...
Rattus norvegicus
J. Steroid Biochem. Mol. Biol.
104
293-300
2007
-
1
-
-
-
-
-
-
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-
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1
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-
1
-
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-
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-
-
-
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1
-
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-
-
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-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
684437
Nicolau-Solano
Relationship between carcass w ...
Sus scrofa
Animal
1
1053-1059
2007
-
1
-
-
-
-
1
-
-
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1
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2
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1
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1
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-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685186
Yang
Rv1106c from Mycobacterium tub ...
Mycobacterium tuberculosis
Biochemistry
46
9058-9067
2007
-
-
1
-
1
-
2
4
1
-
-
-
-
1
-
-
1
1
-
-
-
-
3
-
-
-
-
2
1
-
-
-
2
-
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-
-
1
-
-
1
-
-
2
2
4
1
-
-
-
-
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-
1
-
-
-
-
3
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
686588
Knapczyk
The presence of 3beta-hydroxys ...
Sus scrofa
Exp. Clin. Endocrinol. Diabetes
115
411-416
2007
-
-
-
-
-
-
-
-
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1
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2
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2
-
-
-
-
-
-
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-
-
-
-
-
-
688664
Kim
Differential expression of 3be ...
Rattus norvegicus
J. Reprod. Dev.
53
465-471
2007
2
-
-
-
-
-
5
-
-
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1
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1
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2
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5
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1
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
688685
Thomas
Structure/function of human ty ...
Homo sapiens
J. Steroid Biochem. Mol. Biol.
107
80-87
2007
-
-
-
-
6
-
-
24
-
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-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
22
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
24
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
22
-
-
-
-
-
-
-
-
-
-
688686
Wang
Human 3beta-hydroxysteroid deh ...
Homo sapiens
J. Steroid Biochem. Mol. Biol.
107
88-99
2007
-
1
-
-
1
-
-
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2
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
689697
Herl
Delta5-3beta-hydroxysteroid de ...
Digitalis lanata
Planta Med.
73
704-710
2007
-
-
1
-
-
-
-
8
-
-
1
-
-
1
-
-
1
-
-
1
-
-
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
8
-
-
1
-
-
-
-
1
-
1
-
-
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690002
Szoltys
Expression of androgen recepto ...
Rattus norvegicus
Reprod. Fertil. Dev.
19
356-365
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
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-
1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667452
Sun
Characterization of the Rana g ...
Rana grylio iridovirus, Rana grylio iridovirus 9506
Biochem. Biophys. Res. Commun.
351
44-50
2006
-
-
1
-
-
-
-
-
-
-
-
2
-
2
-
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-
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4
-
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1
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1
1
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-
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2
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-
-
-
-
4
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
669061
Nicolau-Solano
Relationship between the expre ...
Sus scrofa
J. Anim. Sci.
84
2809-2817
2006
-
-
1
-
-
-
1
-
1
-
-
2
-
1
-
-
-
-
-
4
1
-
3
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
-
1
-
-
2
-
-
-
-
-
4
1
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
670027
Kowalewski
Characterization of the canine ...
Canis lupus familiaris
J. Steroid Biochem. Mol. Biol.
101
254-262
2006
-
-
1
-
-
-
-
-
-
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1
-
1
-
-
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-
-
2
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-
1
-
-
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-
-
-
-
-
-
-
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1
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-
-
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-
-
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-
1
-
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-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670028
Pletnev
Rational proteomics V: structu ...
Homo sapiens
J. Steroid Biochem. Mol. Biol.
101
50-60
2006
-
-
1
-
6
-
-
-
-
-
-
1
-
4
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686344
Yarim
Immunolocalization of 3beta-hy ...
Ovis aries
Domest. Anim. Endocrinol.
30
17-27
2006
-
1
-
-
-
-
-
-
-
-
-
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1
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-
-
-
1
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-
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-
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-
1
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-
-
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-
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688674
Malouitre
Regulation of hepatic steroid ...
Rattus norvegicus
J. Steroid Biochem. Mol. Biol.
101
97-105
2006
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
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-
-
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-
1
-
-
-
-
-
-
-
-
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
662371
Thomas
Identification of key amino ac ...
Homo sapiens
J. Biol. Chem.
280
21321-21328
2005
-
-
1
-
3
-
2
13
-
-
-
-
-
3
-
-
1
-
-
5
1
-
4
-
1
-
-
12
1
-
-
2
-
-
-
-
-
2
4
-
4
-
-
4
-
14
-
-
-
-
-
-
-
2
-
5
2
-
4
-
2
-
-
12
2
-
-
-
-
-
-
-
-
-
668413
Nakamura
3beta-hydroxysteroid dehydroge ...
Homo sapiens
Endocr. J.
52
111-115
2005
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
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-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668423
Martin
The human 3beta-hydroxysteroid ...
Homo sapiens
Endocrinology
146
861-869
2005
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
5
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
5
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670036
Lacey
Dose-response effects of phyto ...
Homo sapiens
J. Steroid Biochem. Mol. Biol.
96
279-286
2005
-
-
-
-
-
-
2
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670037
Durocher
Characterization of the guinea ...
Cavia porcellus
J. Steroid Biochem. Mol. Biol.
97
289-298
2005
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656577
Thomas
Serine 124 completes the Tyr, ...
Homo sapiens
J. Mol. Endocrinol.
33
253-261
2004
-
-
1
-
2
-
-
4
-
-
-
1
-
2
-
-
1
-
-
1
-
-
1
-
-
-
-
2
1
1
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
4
-
-
-
1
-
-
-
1
-
1
-
-
1
-
-
-
-
2
1
1
-
-
-
-
-
-
-
-
656657
Ohno
Flavonoid inhibition of overex ...
Homo sapiens
J. Steroid Biochem. Mol. Biol.
88
175-182
2004
-
-
1
-
-
-
13
1
-
-
-
1
-
3
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
1
-
-
-
-
-
13
3
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656788
Thomas
Structure/function aspects of ...
Homo sapiens
Mol. Cell. Endocrinol.
215
73-82
2004
-
-
1
-
10
-
1
6
-
-
1
1
-
1
-
-
1
-
-
3
-
-
1
1
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
10
-
-
1
2
6
-
-
1
1
-
-
-
1
-
3
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656586
Coirini
Characterization and regulatio ...
Rattus norvegicus
J. Neurochem.
84
119-126
2003
-
-
-
-
-
-
5
1
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
1
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656652
Coirini
3 beta-hydroxysteroid dehydrog ...
Rattus norvegicus
J. Steroid Biochem. Mol. Biol.
85
89-94
2003
-
-
1
-
-
-
3
3
-
-
1
3
-
1
-
-
-
-
-
1
-
-
3
1
1
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
3
-
3
-
-
1
3
-
-
-
-
-
1
-
-
3
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
656655
McVey
Inhibition of rat testis micro ...
Rattus norvegicus
J. Steroid Biochem. Mol. Biol.
86
99-105
2003
-
-
-
-
-
-
2
2
1
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
1
2
-
-
-
-
-
1
-
-
-
-
2
2
2
1
-
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
655345
Thomas
Differences in substrate and i ...
Homo sapiens
Endocr. Res.
28
471-475
2002
-
-
1
-
3
-
1
3
-
-
-
1
-
2
-
-
1
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
3
-
-
1
2
3
-
-
-
1
-
-
-
1
-
4
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656091
Thomas
Structure/function relationshi ...
Homo sapiens
J. Biol. Chem.
277
42795-42801
2002
-
-
1
-
6
-
1
13
-
-
-
7
-
4
-
-
1
1
-
2
1
-
10
-
1
-
-
7
2
2
-
1
4
-
-
-
-
3
2
-
11
-
-
3
5
16
-
-
-
7
-
-
-
1
-
2
2
-
10
-
2
-
-
9
3
3
-
-
-
-
-
-
-
-
657352
Qujeq
Development of a quantitative ...
Rattus norvegicus
Steroids
67
1071-1077
2002
-
-
-
-
-
-
-
2
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
1
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
1
-
-
1
-
1
1
-
-
1
1
-
-
-
-
-
-
-
-
389386
Thomas
The engineered, cytosolic form ...
Homo sapiens
J. Mol. Endocrinol.
27
77-83
2001
-
-
1
1
-
-
-
4
-
-
1
1
-
1
-
-
-
-
-
1
1
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
4
-
-
1
1
-
-
-
-
-
1
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389382
Pozzi
Kinetic properties of microsom ...
Rhinella arenarum
J. Steroid Biochem. Mol. Biol.
73
257-264
2000
-
-
-
-
-
-
1
2
1
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-
2
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
2
1
-
-
2
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389385
Huang
Membrane-bound human 3beta-hyd ...
Homo sapiens
Protein Expr. Purif.
18
169-174
2000
-
-
1
-
-
-
-
1
2
-
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1
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2
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-
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389389
Suzuki
3beta-Hydroxysteroid dehydroge ...
Homo sapiens
J. Clin. Endocrinol.
85
3669-3672
2000
-
-
-
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2
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2
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2
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3
-
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-
389387
Thomas
Creation of a fully active, cy ...
Homo sapiens
J. Mol. Endocrinol.
23
231-239
1999
-
-
1
-
1
-
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1
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1
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4
3
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2
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1
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1
1
-
1
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1
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4
3
-
2
-
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-
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389390
Wong
Bovine adrenal 3beta-hydroxyst ...
Bos taurus, Cricetinae
J. Steroid Biochem. Mol. Biol.
71
191-202
1999
-
-
-
-
-
-
9
4
1
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2
-
2
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1
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6
-
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4
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2
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2
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9
-
4
1
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2
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1
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-
6
-
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-
4
-
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-
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-
-
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-
-
389402
Finsterbusch
DELTA5-3beta-Hydroxysteroid de ...
Digitalis lanata, Digitalis lanata L.
Planta
209
478-486
1999
-
-
-
-
-
-
-
4
-
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1
2
-
3
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1
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1
1
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8
1
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3
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3
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4
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1
2
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1
-
1
1
-
8
1
-
-
-
-
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-
-
-
-
-
-
-
-
389384
Abbaszade
Isolation of a new mouse 3beta ...
Mus musculus
Endocrinology
138
1392-1399
1997
-
-
1
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-
2
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1
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1
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5
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3
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5
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3
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389392
Furster
Purification of a 3beta-hydrox ...
Sus scrofa
J. Biol. Chem.
271
20903-20907
1996
-
-
-
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-
2
1
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1
1
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3
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1
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1
5
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6
1
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1
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1
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1
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1
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1
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1
5
-
6
1
-
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-
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-
389400
Rogerson
Expression and characterizatio ...
Cricetinae
J. Steroid Biochem. Mol. Biol.
55
481-487
1995
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1
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4
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2
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3
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4
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3
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2
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389391
Cherradi
Characterization of the 3beta- ...
Bos taurus
Endocrinology
134
1358-1364
1994
-
-
-
-
-
-
-
4
1
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-
2
-
1
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1
2
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3
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1
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1
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4
1
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1
2
-
3
-
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389394
Nakajin
Purification and characterizat ...
Sus scrofa
Biol. Pharm. Bull.
17
1155-1160
1994
-
-
-
-
-
-
12
35
1
-
2
1
-
1
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1
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1
1
-
38
1
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1
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1
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12
-
35
1
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2
1
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1
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1
1
-
38
1
-
-
-
-
-
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-
-
-
-
-
-
-
-
389397
Sakai
Ovarian 3beta-hydroxysteroid d ...
Oncorhynchus mykiss
FEBS Lett.
350
309-313
1994
-
-
1
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-
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1
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1
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1
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1
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2
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-
389401
Nash
Over-expression of human type ...
Homo sapiens
J. Steroid Biochem. Mol. Biol.
50
235-240
1994
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1
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4
2
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2
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1
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1
4
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3
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1
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1
1
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4
2
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2
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1
4
-
3
-
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-
389383
Clarke
Enzyme characteristics of two ...
Mus musculus
Endocrinology
132
1971-1976
1993
-
-
1
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-
8
-
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2
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1
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5
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4
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1
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1
1
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8
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5
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4
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389371
Thomas
Affinity labeling of human pla ...
Homo sapiens
Biochemistry
31
5522-5527
1992
-
-
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-
1
-
1
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2
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1
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1
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3
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2
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1
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3
-
-
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-
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-
389388
Riley
Immunohistochemical localizati ...
Homo sapiens
J. Clin. Endocrinol.
75
956-961
1992
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-
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1
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1
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1
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2
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2
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1
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1
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1
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1
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2
-
-
2
-
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-
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389393
Hebert
Kinetic evidence for separate ...
Sus scrofa
J. Steroid Biochem. Mol. Biol.
42
901-910
1992
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1
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1
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4
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1
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1
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1
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2
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1
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-
4
-
-
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-
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-
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-
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389395
Labrie
Structure and tissue-specific ...
Homo sapiens, Rattus norvegicus
J. Steroid Biochem. Mol. Biol.
41
421-435
1992
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2
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9
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4
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2
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2
2
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2
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9
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2898
Simard
Characterization of macaque 3b ...
Macaca mulatta
Mol. Cell. Endocrinol.
75
101-110
1991
-
-
-
-
-
-
-
-
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1
1
-
1
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1
1
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1
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1
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1
1
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-
1
1
-
-
-
-
-
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-
-
-
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-
-
-
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389396
Lorence
3beta-Hydroxysteroid dehydroge ...
Rattus norvegicus
Mol. Cell. Endocrinol.
80
21-31
1991
-
-
1
-
-
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-
2
-
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-
2
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1
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1
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2
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1
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1
1
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2
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2
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1
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-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389399
Perry
Subcellular distribution of 3b ...
Bos taurus, Mus musculus, Rattus norvegicus
Steroids
56
329-336
1991
-
-
-
-
-
-
-
-
5
-
-
3
-
3
-
-
-
-
-
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-
3
-
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-
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-
3
-
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3
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5
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3
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-
3
-
-
-
-
-
-
-
-
-
-
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-
-
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389378
Ishii-Ohba
Testicular and adrenal 3beta-h ...
Rattus norvegicus
J. Steroid Biochem.
27
775-779
1987
-
-
-
-
-
-
-
-
-
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-
1
-
1
-
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-
-
2
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2
1
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2
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-
2
-
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-
1
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2
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
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389379
Ishii-Ohba
Purification and properties of ...
Rattus norvegicus
J. Steroid Biochem.
25
555-560
1986
-
-
-
-
-
-
1
-
1
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-
1
-
1
-
-
1
-
-
1
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-
2
1
-
-
-
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-
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-
1
-
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-
1
-
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-
-
1
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-
1
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1
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1
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
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389369
Hiwatashi
Purification and kinetic prope ...
Bos taurus
J. Biochem.
98
1519-1526
1985
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-
-
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5
4
1
-
1
1
-
1
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-
1
-
-
1
1
1
2
1
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
5
-
4
1
-
1
1
-
-
-
1
-
1
1
1
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
723061
Owen
The degradation of beta-sitost ...
Pseudomonas sp. 10590, Pseudomonas sp.
J. Steroid Biochem.
23
327-332
1985
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2
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2
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-
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-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389380
Spona
Differences in inhibition by v ...
Comamonas testosteroni, Rattus norvegicus
Endocrinol. Exp.
17
107-118
1983
-
-
-
-
-
-
6
2
2
-
-
4
-
4
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
6
-
2
2
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-
4
-
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-
-
-
1
-
-
6
-
-
-
-
-
-
-
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-
389372
Rabe
Partial characterization of pl ...
Homo sapiens
J. Steroid Biochem.
17
427-433
1982
-
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7
2
2
1
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1
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2
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-
-
-
2
-
-
2
-
1
1
1
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
7
-
2
2
1
-
1
-
-
-
-
-
2
-
-
2
-
1
1
1
-
1
-
-
-
-
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-
-
-
-
389373
Armstrong
Properties of 5-ene-3beta-hydr ...
Gallus gallus
J. Steroid Biochem.
17
225-230
1982
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5
1
1
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1
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-
1
-
-
2
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
5
-
1
1
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
389370
Shikita
Preparation of highly purified ...
Pseudomonas sp., Pseudomonas sp. TB
Anal. Biochem.
95
286-292
1979
-
-
-
-
-
-
-
-
-
-
-
4
-
2
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-
1
-
-
-
1
1
8
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
1
-
-
1
1
8
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
389374
Vincent
Role of thiol group(s) in the ...
Bos taurus
Biochim. Biophys. Acta
525
1-8
1978
2
-
-
-
-
-
3
1
1
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
1
-
-
-
-
3
-
1
1
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389381
Kerenyi
Properties of delta5-3beta-hyd ...
Streptomyces griseocarneus
Acta Microbiol. Acad. Sci. Hung.
22
487-496
1975
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
3
-
1
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1
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-
-
2884
Ford
Purification and properties of ...
Ovis aries
J. Biol. Chem.
249
1363-1368
1974
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1
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2
1
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1
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1
-
-
-
1
-
2
1
-
-
-
-
-
-
-
1
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389376
Cheatum
Purification and properties of ...
Bos taurus
Biochim. Biophys. Acta
122
1-13
1966
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389377
Koritz
The conversion of pregnenolone ...
Rattus norvegicus
Biochemistry
3
1098-1102
1964
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