BRENDA - Enzyme Database show
show all sequences of 1.1.1.145

Identification of key amino acids responsible for the substantially higher affinities of human type 1 3beta-hydroxysteroid dehydrogenase/isomerase (3b-HSD1) for substrates, coenzymes, and inhibitors relative to human 3b-HSD2

Thomas, J.L.; Boswell, E.L.; Scaccia, L.A.; Pletnev, V.; Umland, T.C.; J. Biol. Chem. 280, 21321-21328 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression of soluble wild-type and mutant isozyme 3beta-HSD1 in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system; expression of soluble wild-type and mutant isozyme 3beta-HSD2 in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system
Homo sapiens
Engineering
Amino acid exchange
Commentary
Organism
Q105M
site-directed mutagenesis of isozyme 1, the mutants show altered kinetics compared to the wild-type isozymes; site-directed mutagenesis of isozyme 2, the mutants show altered kinetics compared to the wild-type isozymes
Homo sapiens
Q240R
site-directed mutagenesis of isozyme 1, the mutant shows altered kinetics compared to the wild-type isozyme
Homo sapiens
R240Q
site-directed mutagenesis of isozyme 2, the mutant shows altered kinetics compared to the wild-type isozyme
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
epostane
;
Homo sapiens
trilostane
;
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics, recombinant wild-type and mutant isozymes, overview, kinetics for the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1, activity; kinetics, recombinant wild-type and mutant isozymes, overview, kinetics for the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1, activity
Homo sapiens
0.0037
-
dehydroepiandrosterone
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD1
Homo sapiens
0.0061
-
dehydroepiandrosterone
pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q240R
Homo sapiens
0.025
-
dehydroepiandrosterone
pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant Q105M
Homo sapiens
0.0256
-
NAD+
pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q240R
Homo sapiens
0.0327
-
dehydroepiandrosterone
pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant R240Q
Homo sapiens
0.0341
-
NAD+
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD1
Homo sapiens
0.0372
-
NAD+
pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant R240Q
Homo sapiens
0.0388
-
NAD+
pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q105M
Homo sapiens
0.0405
-
dehydroepiandrosterone
pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q105M
Homo sapiens
0.0473
-
dehydroepiandrosterone
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD2
Homo sapiens
0.0614
-
NAD+
pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant Q105M
Homo sapiens
0.0863
-
NAD+
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD2
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
P14060
3beta-HSD1; isozyme 3beta-HSD1, bifunctional enzyme
-
Homo sapiens
P26439
3beta-HSD2; isozyme 3beta-HSD2, bifunctional enzyme
-
Purification (Commentary)
Commentary
Organism
recombinant soluble wild-type and mutant isozyme 3beta-HSD1 from Sf9 insect cells; recombinant soluble wild-type and mutant isozyme 3beta-HSD2 from Sf9 insect cells
Homo sapiens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
adrenal gland
isozyme 3beta-HSD2
Homo sapiens
-
breast cancer cell
isozyme 3beta-HSD1
Homo sapiens
-
gonad
isozyme 3beta-HSD2
Homo sapiens
-
placenta
isozyme 3beta-HSD1
Homo sapiens
-
prostate tumor cell
isozyme 3beta-HSD1
Homo sapiens
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
;
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dehydroepiandrosterone + NAD+
-
662371
Homo sapiens
?
-
-
-
r
additional information
bifunctional enzyme that also catalyzes the reaction of the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1
662371
Homo sapiens
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
27
-
assay at; assay at
Homo sapiens
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.022
-
NAD+
pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q240R
Homo sapiens
0.023
-
dehydroepiandrosterone
pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q240R
Homo sapiens
0.038
-
NAD+
pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q105M
Homo sapiens
0.055
-
dehydroepiandrosterone
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD1
Homo sapiens
0.058
-
NAD+
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD1
Homo sapiens
0.075
-
dehydroepiandrosterone
pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant Q105M
Homo sapiens
0.113
-
dehydroepiandrosterone
pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q105M
Homo sapiens
0.118
-
dehydroepiandrosterone
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD2
Homo sapiens
0.118
-
NAD+
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD2
Homo sapiens
0.145
-
NAD+
pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant Q105M
Homo sapiens
0.187
-
NAD+
pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant R240Q
Homo sapiens
0.217
-
dehydroepiandrosterone
pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant R240Q
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at; assay at
Homo sapiens
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
;
Homo sapiens
NADH
;
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of soluble wild-type and mutant isozyme 3beta-HSD1 in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system
Homo sapiens
expression of soluble wild-type and mutant isozyme 3beta-HSD2 in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system
Homo sapiens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Homo sapiens
NADH
-
Homo sapiens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
Q105M
site-directed mutagenesis of isozyme 1, the mutants show altered kinetics compared to the wild-type isozymes
Homo sapiens
Q105M
site-directed mutagenesis of isozyme 2, the mutants show altered kinetics compared to the wild-type isozymes
Homo sapiens
Q240R
site-directed mutagenesis of isozyme 1, the mutant shows altered kinetics compared to the wild-type isozyme
Homo sapiens
R240Q
site-directed mutagenesis of isozyme 2, the mutant shows altered kinetics compared to the wild-type isozyme
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
epostane
-
Homo sapiens
trilostane
-
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics, recombinant wild-type and mutant isozymes, overview, kinetics for the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1, activity
Homo sapiens
0.0037
-
dehydroepiandrosterone
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD1
Homo sapiens
0.0061
-
dehydroepiandrosterone
pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q240R
Homo sapiens
0.025
-
dehydroepiandrosterone
pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant Q105M
Homo sapiens
0.0256
-
NAD+
pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q240R
Homo sapiens
0.0327
-
dehydroepiandrosterone
pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant R240Q
Homo sapiens
0.0341
-
NAD+
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD1
Homo sapiens
0.0372
-
NAD+
pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant R240Q
Homo sapiens
0.0388
-
NAD+
pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q105M
Homo sapiens
0.0405
-
dehydroepiandrosterone
pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q105M
Homo sapiens
0.0473
-
dehydroepiandrosterone
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD2
Homo sapiens
0.0614
-
NAD+
pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant Q105M
Homo sapiens
0.0863
-
NAD+
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD2
Homo sapiens
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant soluble wild-type and mutant isozyme 3beta-HSD1 from Sf9 insect cells
Homo sapiens
recombinant soluble wild-type and mutant isozyme 3beta-HSD2 from Sf9 insect cells
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
adrenal gland
isozyme 3beta-HSD2
Homo sapiens
-
breast cancer cell
isozyme 3beta-HSD1
Homo sapiens
-
gonad
isozyme 3beta-HSD2
Homo sapiens
-
placenta
isozyme 3beta-HSD1
Homo sapiens
-
prostate tumor cell
isozyme 3beta-HSD1
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dehydroepiandrosterone + NAD+
-
662371
Homo sapiens
?
-
-
-
r
additional information
bifunctional enzyme that also catalyzes the reaction of the DELTA-5-3-ketosteroid isomerase, EC 5.3.3.1
662371
Homo sapiens
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
27
-
assay at
Homo sapiens
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.022
-
NAD+
pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q240R
Homo sapiens
0.023
-
dehydroepiandrosterone
pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q240R
Homo sapiens
0.038
-
NAD+
pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q105M
Homo sapiens
0.055
-
dehydroepiandrosterone
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD1
Homo sapiens
0.058
-
NAD+
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD1
Homo sapiens
0.075
-
dehydroepiandrosterone
pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant Q105M
Homo sapiens
0.113
-
dehydroepiandrosterone
pH 7.4, 27°C, recombinant isozyme 3beta-HSD1 mutant Q105M
Homo sapiens
0.118
-
dehydroepiandrosterone
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD2
Homo sapiens
0.118
-
NAD+
pH 7.4, 27°C, recombinant wild-type isozyme 3beta-HSD2
Homo sapiens
0.145
-
NAD+
pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant Q105M
Homo sapiens
0.187
-
NAD+
pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant R240Q
Homo sapiens
0.217
-
dehydroepiandrosterone
pH 7.4, 27°C, recombinant isozyme 3beta-HSD2 mutant R240Q
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Homo sapiens
Other publictions for EC 1.1.1.145
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725807
Wojciechowicz
The activity and localization ...
Sus scrofa
J. Reprod. Dev.
59
49-58
2013
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1
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2
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2
-
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2
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725511
Prasad
Mitochondrial 3beta-hydroxyste ...
Homo sapiens
J. Biol. Chem.
287
9534-9546
2012
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1
-
-
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-
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1
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1
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1
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1
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1
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2
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1
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1
1
1
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1
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1
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1
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1
1
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2
-
1
-
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-
1
1
1
-
-
-
-
-
-
-
725931
Rasmussen
Expression of hepatic 3beta-hy ...
Sus scrofa
Mol. Biol. Rep.
39
7927-32
2012
-
1
-
-
-
-
-
-
-
-
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1
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1
-
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1
-
-
724302
Rajapaksha
Lipid-mediated unfolding of 3b ...
Homo sapiens
Biochemistry
50
11015-11024
2011
-
-
1
-
-
-
1
-
1
-
1
1
-
1
-
-
-
-
1
-
-
-
3
-
1
-
3
-
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1
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1
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1
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1
1
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1
-
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3
-
1
-
3
-
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-
-
-
-
-
-
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724518
Thomas
Inhibition of the M. tuberculo ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Bioorg. Med. Chem. Lett.
21
2216-2219
2011
-
-
-
-
-
-
20
-
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-
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2
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2
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1
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1
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20
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20
20
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2
-
1
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1
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-
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725447
Pawlak
Inner mitochondrial translocas ...
Sus scrofa
J. Biol. Chem.
286
39130-39140
2011
-
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1
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1
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1
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1
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1
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-
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1
1
-
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725612
Zhao
Homology modeling and molecula ...
Homo sapiens
J. Comput. Chem.
32
33-42
2011
-
-
-
-
1
-
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-
-
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3
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2
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2
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1
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725816
Nakamura
Human adrenal cells that expre ...
Homo sapiens
J. Steroid Biochem. Mol. Biol.
123
122-126
2011
-
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-
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1
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2
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1
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1
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725820
Thomas
Selective inhibition of human ...
Homo sapiens
J. Steroid Biochem. Mol. Biol.
125
57-65
2011
-
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1
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3
-
2
7
-
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2
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2
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7
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9
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2
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5
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4
9
7
-
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2
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7
-
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-
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-
5
6
713188
Nittono
3?-hydroxy-??-C??-steroid dehy ...
Homo sapiens
Pediatr. Internat.
52
e192-e195
2010
-
1
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1
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1
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713329
Ernst
Expression of 3beta-HSD and P5 ...
Digitalis lanata, Digitalis lanata EHRH
Planta Med.
76
923-927
2010
-
-
-
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2
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2
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4
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The functions of key residues ...
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6
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2
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29
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7
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Lysiak
Hypoxia-inducible factor-1alph ...
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The (+)- and (-)-gossypols pot ...
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700182
Thomas
Structural basis for the selec ...
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1
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1
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1
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3
2
2
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1
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3
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1
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2
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Matsunaga
Characterization of human DHRS ...
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43
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35
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3
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4
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3
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1
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4
6
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37
1
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1
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43
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35
2
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687967
Welzel
Carboxyl-terminal mutations in ...
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693887
Thomas
Structure/function of the inhi ...
Homo sapiens
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1
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1
8
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1
1
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2
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2
8
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1
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5
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-
1
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699111
Parthasarathy
Effects of corticosterone defi ...
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1
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-
1
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-
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700557
Fernandez Presas
The key steroidogenic enzyme 3 ...
Taenia crassiceps, Taenia crassiceps WFU, Taenia solium
Parasitol. Res.
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2
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2
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2
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9
-
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-
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681643
Meffre
3beta-Hydroxysteroid dehydroge ...
Rattus norvegicus
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293-300
2007
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1
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1
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684437
Nicolau-Solano
Relationship between carcass w ...
Sus scrofa
Animal
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2007
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1
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1
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2
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-
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-
-
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685186
Yang
Rv1106c from Mycobacterium tub ...
Mycobacterium tuberculosis
Biochemistry
46
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1
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1
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2
4
1
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1
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1
1
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3
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-
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2
1
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-
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2
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1
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1
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2
2
4
1
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1
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-
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-
3
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-
-
-
2
1
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686588
Knapczyk
The presence of 3beta-hydroxys ...
Sus scrofa
Exp. Clin. Endocrinol. Diabetes
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2007
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2
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688664
Kim
Differential expression of 3be ...
Rattus norvegicus
J. Reprod. Dev.
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2
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5
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1
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688685
Thomas
Structure/function of human ty ...
Homo sapiens
J. Steroid Biochem. Mol. Biol.
107
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6
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24
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1
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22
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688686
Wang
Human 3beta-hydroxysteroid deh ...
Homo sapiens
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2007
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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689697
Herl
Delta5-3beta-hydroxysteroid de ...
Digitalis lanata
Planta Med.
73
704-710
2007
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1
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8
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1
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1
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1
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1
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8
1
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-
-
-
-
-
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-
-
-
-
-
-
1
-
-
-
-
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8
-
-
1
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-
1
-
1
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-
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690002
Szoltys
Expression of androgen recepto ...
Rattus norvegicus
Reprod. Fertil. Dev.
19
356-365
2007
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1
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1
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-
1
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-
-
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-
-
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667452
Sun
Characterization of the Rana g ...
Rana grylio iridovirus, Rana grylio iridovirus 9506
Biochem. Biophys. Res. Commun.
351
44-50
2006
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1
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1
1
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-
2
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-
4
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669061
Nicolau-Solano
Relationship between the expre ...
Sus scrofa
J. Anim. Sci.
84
2809-2817
2006
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4
1
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3
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1
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1
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-
1
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-
1
1
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-
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-
1
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-
1
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2
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4
1
-
3
-
1
-
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1
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670027
Kowalewski
Characterization of the canine ...
Canis lupus familiaris
J. Steroid Biochem. Mol. Biol.
101
254-262
2006
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1
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1
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2
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1
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-
-
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670028
Pletnev
Rational proteomics V: structu ...
Homo sapiens
J. Steroid Biochem. Mol. Biol.
101
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2006
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1
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1
1
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1
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6
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1
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1
1
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-
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686344
Yarim
Immunolocalization of 3beta-hy ...
Ovis aries
Domest. Anim. Endocrinol.
30
17-27
2006
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1
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1
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1
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-
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-
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688674
Malouitre
Regulation of hepatic steroid ...
Rattus norvegicus
J. Steroid Biochem. Mol. Biol.
101
97-105
2006
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1
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1
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1
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-
-
-
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662371
Thomas
Identification of key amino ac ...
Homo sapiens
J. Biol. Chem.
280
21321-21328
2005
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3
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2
13
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1
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5
1
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4
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1
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12
1
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2
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2
4
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4
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4
-
14
-
-
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2
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5
2
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4
-
2
-
-
12
2
-
-
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668413
Nakamura
3beta-hydroxysteroid dehydroge ...
Homo sapiens
Endocr. J.
52
111-115
2005
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1
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1
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1
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2
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1
-
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-
-
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668423
Martin
The human 3beta-hydroxysteroid ...
Homo sapiens
Endocrinology
146
861-869
2005
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1
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1
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1
1
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1
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5
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2
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670036
Lacey
Dose-response effects of phyto ...
Homo sapiens
J. Steroid Biochem. Mol. Biol.
96
279-286
2005
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1
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1
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1
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1
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2
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-
1
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1
-
-
1
-
-
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670037
Durocher
Characterization of the guinea ...
Cavia porcellus
J. Steroid Biochem. Mol. Biol.
97
289-298
2005
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3
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1
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1
1
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3
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3
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656577
Thomas
Serine 124 completes the Tyr, ...
Homo sapiens
J. Mol. Endocrinol.
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253-261
2004
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1
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2
1
1
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1
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2
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Flavonoid inhibition of overex ...
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1
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10
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6
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1
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Characterization and regulatio ...
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1
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1
1
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1
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3
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1
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3
1
1
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1
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1
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2
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2
2
1
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1
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2
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1
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3
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3
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1
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1
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2
1
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10
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1
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7
2
2
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1
4
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3
2
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11
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3
5
16
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7
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1
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2
2
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10
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2
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9
3
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657352
Qujeq
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Rattus norvegicus
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1
1
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1
1
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2
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1
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1
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1
-
1
1
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1
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389386
Thomas
The engineered, cytosolic form ...
Homo sapiens
J. Mol. Endocrinol.
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1
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2
1
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1
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1
1
1
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4
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1
1
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1
1
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2
1
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389382
Pozzi
Kinetic properties of microsom ...
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73
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3
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1
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1
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1
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2
1
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2
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1
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3
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-
-
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389385
Huang
Membrane-bound human 3beta-hyd ...
Homo sapiens
Protein Expr. Purif.
18
169-174
2000
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1
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1
2
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1
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1
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1
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1
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2
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1
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1
1
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-
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1
2
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1
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1
-
-
1
-
2
-
-
-
-
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389389
Suzuki
3beta-Hydroxysteroid dehydroge ...
Homo sapiens
J. Clin. Endocrinol.
85
3669-3672
2000
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2
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3
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1
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1
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2
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2
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-
3
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389387
Thomas
Creation of a fully active, cy ...
Homo sapiens
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23
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1999
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1
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1
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4
3
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2
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1
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1
1
-
1
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1
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-
4
3
-
2
-
-
-
-
-
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389390
Wong
Bovine adrenal 3beta-hydroxyst ...
Bos taurus, Cricetinae
J. Steroid Biochem. Mol. Biol.
71
191-202
1999
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6
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4
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2
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2
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9
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4
1
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2
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1
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6
-
-
-
-
4
-
-
-
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-
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389402
Finsterbusch
DELTA5-3beta-Hydroxysteroid de ...
Digitalis lanata, Digitalis lanata L.
Planta
209
478-486
1999
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4
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2
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1
1
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8
1
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3
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-
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-
3
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-
4
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1
2
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1
-
1
1
-
8
1
-
-
-
-
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-
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389384
Abbaszade
Isolation of a new mouse 3beta ...
Mus musculus
Endocrinology
138
1392-1399
1997
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1
1
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5
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3
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389392
Furster
Purification of a 3beta-hydrox ...
Sus scrofa
J. Biol. Chem.
271
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1996
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6
1
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1
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1
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2
1
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1
1
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1
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1
5
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6
1
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389400
Rogerson
Expression and characterizatio ...
Cricetinae
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55
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1995
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1
1
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3
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2
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389391
Cherradi
Characterization of the 3beta- ...
Bos taurus
Endocrinology
134
1358-1364
1994
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4
1
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1
2
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3
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1
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1
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4
1
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2
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1
2
-
3
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389394
Nakajin
Purification and characterizat ...
Sus scrofa
Biol. Pharm. Bull.
17
1155-1160
1994
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12
35
1
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2
1
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1
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1
1
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38
1
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1
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1
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12
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35
1
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2
1
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1
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1
1
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38
1
-
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-
-
-
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-
-
-
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-
-
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389397
Sakai
Ovarian 3beta-hydroxysteroid d ...
Oncorhynchus mykiss
FEBS Lett.
350
309-313
1994
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1
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1
1
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1
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1
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389401
Nash
Over-expression of human type ...
Homo sapiens
J. Steroid Biochem. Mol. Biol.
50
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1994
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1
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1
1
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4
2
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2
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1
4
-
3
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389383
Clarke
Enzyme characteristics of two ...
Mus musculus
Endocrinology
132
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1993
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1
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8
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8
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4
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389371
Thomas
Affinity labeling of human pla ...
Homo sapiens
Biochemistry
31
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1992
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3
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2
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2
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2
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1
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3
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389388
Riley
Immunohistochemical localizati ...
Homo sapiens
J. Clin. Endocrinol.
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1992
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2
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389393
Hebert
Kinetic evidence for separate ...
Sus scrofa
J. Steroid Biochem. Mol. Biol.
42
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1992
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4
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389395
Labrie
Structure and tissue-specific ...
Homo sapiens, Rattus norvegicus
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41
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1992
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2
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9
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2898
Simard
Characterization of macaque 3b ...
Macaca mulatta
Mol. Cell. Endocrinol.
75
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1991
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1
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1
1
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1
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389396
Lorence
3beta-Hydroxysteroid dehydroge ...
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Mol. Cell. Endocrinol.
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1991
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1
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1
1
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2
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2
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1
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2
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389399
Perry
Subcellular distribution of 3b ...
Bos taurus, Mus musculus, Rattus norvegicus
Steroids
56
329-336
1991
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5
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3
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3
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3
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3
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3
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5
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3
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3
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-
-
-
-
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-
-
-
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389378
Ishii-Ohba
Testicular and adrenal 3beta-h ...
Rattus norvegicus
J. Steroid Biochem.
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775-779
1987
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389379
Ishii-Ohba
Purification and properties of ...
Rattus norvegicus
J. Steroid Biochem.
25
555-560
1986
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389369
Hiwatashi
Purification and kinetic prope ...
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J. Biochem.
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1519-1526
1985
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723061
Owen
The degradation of beta-sitost ...
Pseudomonas sp. 10590, Pseudomonas sp.
J. Steroid Biochem.
23
327-332
1985
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389380
Spona
Differences in inhibition by v ...
Comamonas testosteroni, Rattus norvegicus
Endocrinol. Exp.
17
107-118
1983
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6
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389372
Rabe
Partial characterization of pl ...
Homo sapiens
J. Steroid Biochem.
17
427-433
1982
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2
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389373
Armstrong
Properties of 5-ene-3beta-hydr ...
Gallus gallus
J. Steroid Biochem.
17
225-230
1982
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389370
Shikita
Preparation of highly purified ...
Pseudomonas sp., Pseudomonas sp. TB
Anal. Biochem.
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286-292
1979
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389374
Vincent
Role of thiol group(s) in the ...
Bos taurus
Biochim. Biophys. Acta
525
1-8
1978
2
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389381
Kerenyi
Properties of delta5-3beta-hyd ...
Streptomyces griseocarneus
Acta Microbiol. Acad. Sci. Hung.
22
487-496
1975
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2884
Ford
Purification and properties of ...
Ovis aries
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1363-1368
1974
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389376
Cheatum
Purification and properties of ...
Bos taurus
Biochim. Biophys. Acta
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1-13
1966
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389377
Koritz
The conversion of pregnenolone ...
Rattus norvegicus
Biochemistry
3
1098-1102
1964
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2
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