Literature summary for 1.1.1.145 extracted from

Nash, W.E.; Mercer, R.W.; Blanco, G.; Strickler, R.C.; Mason, J.I.; Thomas, J.L.
Over-expression of human type I (placental) 3beta-hydroxy-5-ene-steroid dehydrogenase/isomerase in insect cells infected with recombinant baculovirus (1994), J. Steroid Biochem. Mol. Biol., 50, 235-240.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Spodoptera fungiperda insect cells	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0144	-	5-androstene-3,17-dione	placental microsomes	Homo sapiens
0.0147	-	5-androstene-3,17-dione	Sf9 cell homogenate	Homo sapiens
0.0167	-	5alpha-Androstan-3beta-ol-17-one	placental microsomes	Homo sapiens
0.0179	-	5alpha-Androstan-3beta-ol-17-one	Sf9 cell homogenate	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
microsome	-	Homo sapiens	-	-
mitochondrion	-	Homo sapiens	5739	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
dehydroepiandrosterone + NAD+	Homo sapiens	-	androst-5-en-3,17-dione + NADH + H+	-	?
pregnenolone + NAD(P)+	Homo sapiens	-	pregn-5-en-3,20-dione + NAD(P)H + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	3beta-HSD I	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
placenta	3beta-HSD I	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0091	-	placental microsomes, 3beta-HSD activity	Homo sapiens
0.0145	-	Sf9-cells, 3beta-HSD activity	Homo sapiens
0.0172	-	placental microsomes, isomerase activity	Homo sapiens
0.0257	-	Sf9-cells, isomerase activity	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5alpha-androstan-3beta-ol-17-one + NADP+	-	Homo sapiens	5alpha-androstan-3,17-dione + NADPH	-	?
dehydroepiandrosterone + NAD+	-	Homo sapiens	androst-5-en-3,17-dione + NADH + H+	-	?
pregnenolone + NAD(P)+	-	Homo sapiens	pregn-5-en-3,20-dione + NAD(P)H + H+	-	?

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Homo sapiens

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Release 2023.1 (January 2023)