Data extracted from this reference:
Activating Compound
additional information
dehydrogenase is induced by limonene, alpha-pinene, delta-p-menthene and to lesser extend by p-cymene
Pseudomonas sp.
Inhibitors
Ag+
0.005 mM, complete inhibition, prevented by 10 mM glutathione
Pseudomonas sp.
Cu2+
5 mM, complete inhibition, prevented by 5 mM glutathione
Pseudomonas sp.
Hg2+
0.005 mM, complete inhibition, prevented by 10 mM glutathione
Pseudomonas sp.
iodoacetate
10 mM, 95% inhibition, prevented by 10 mM glutathione
Pseudomonas sp.
additional information
glucose represses the induction of the enzyme by limonene
Pseudomonas sp.
NADH
competitive inhibition
Pseudomonas sp.
Ni2+
2 mM, 75% inhibition, complete reversion with 5 mM EDTA
Pseudomonas sp.
o-phenanthroline
at concentrations higher than 3 mM and low concentration of NAD+, about 50% inhibition
Pseudomonas sp.
p-hydroxymercuribenzoate
0.005 mM, complete inhibition, prevented by 10 mM glutathione
Pseudomonas sp.
Zn2+
2 mM, 75% inhibition, complete reversion with 10 mM EDTA
Pseudomonas sp.
KM Value [mM]
additional information
additional information
Pseudomonas sp.
0.077
NAD+
at pH 8.6
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
perillyl alcohol + NAD+
Pseudomonas sp.
catalyzes dehydrogenation reaction which is a part of the catabolic sequence of alpha-pinenes and beta-pinenes, limonene, DELTA1-p-methene and p-cymene
perillyl aldehyde + NADH
Pseudomonas sp.
perillyl alcohol + NAD+
Pseudomonas sp. PL-
catalyzes dehydrogenation reaction which is a part of the catabolic sequence of alpha-pinenes and beta-pinenes, limonene, DELTA1-p-methene and p-cymene
perillyl aldehyde + NADH
Pseudomonas sp. PL-
Organism
Pseudomonas sp.
PL-strain
Pseudomonas sp. PL-
PL-strain
Purification (Commentary)
PL-strain
Pseudomonas sp.
Specific Activity [micromol/min/mg]
13
DEAE-eluate
Pseudomonas sp.
Storage Stability
-20°C, for at least 3 days, DEAE eluate
Pseudomonas sp.
Substrates and Products (Substrate)
additional information
no substrates are p-menthan-7-ol, DELTA1-p-menthen-10-ol, DELTA8-menthen-10-ol, p-menthan-10-ol, myrtenol, isoborneol, borneol, cyclohexenol, o-menthyl benzyl alcohol, phthalyl alcohol, salicylic alcohol, 7-methyl cumic alcohol, ethanol, methanol
285942
Pseudomonas sp.
?
additional information
no substrates are p-menthan-7-ol, DELTA1-p-menthen-10-ol, DELTA8-menthen-10-ol, p-menthan-10-ol, myrtenol, isoborneol, borneol, cyclohexenol, o-menthyl benzyl alcohol, phthalyl alcohol, salicylic alcohol, 7-methyl cumic alcohol, ethanol, methanol
285942
Pseudomonas sp. PL-
?
perillyl alcohol + NAD+
e.g.: 8-hydroxyphellandrol, cumic alcohol
285942
Pseudomonas sp.
perillyl aldehyde + NADH
285942
Pseudomonas sp.
r
perillyl alcohol + NAD+
p-ethyl benzyl alcohol, p-methyl benzyl alcohol
285942
Pseudomonas sp.
perillyl aldehyde + NADH
285942
Pseudomonas sp.
r
perillyl alcohol + NAD+
rate of the reverse reaction is about 20% of the rate of the forward reaction, oxidizes a number of primary alcohols with the alcohol group allylic to an endocyclic double bond and a 6-membered ring, either aromatic or hydroaromatic
285942
Pseudomonas sp.
perillyl aldehyde + NADH
285942
Pseudomonas sp.
r
perillyl alcohol + NAD+
catalyzes dehydrogenation reaction which is a part of the catabolic sequence of alpha-pinenes and beta-pinenes, limonene, DELTA1-p-methene and p-cymene
285942
Pseudomonas sp.
perillyl aldehyde + NADH
285942
Pseudomonas sp.
perillyl alcohol + NAD+
e.g.: 8-hydroxyphellandrol, cumic alcohol
285942
Pseudomonas sp. PL-
perillyl aldehyde + NADH
285942
Pseudomonas sp. PL-
r
perillyl alcohol + NAD+
p-ethyl benzyl alcohol, p-methyl benzyl alcohol
285942
Pseudomonas sp. PL-
perillyl aldehyde + NADH
285942
Pseudomonas sp. PL-
r
perillyl alcohol + NAD+
rate of the reverse reaction is about 20% of the rate of the forward reaction, oxidizes a number of primary alcohols with the alcohol group allylic to an endocyclic double bond and a 6-membered ring, either aromatic or hydroaromatic
285942
Pseudomonas sp. PL-
perillyl aldehyde + NADH
285942
Pseudomonas sp. PL-
r
perillyl alcohol + NAD+
catalyzes dehydrogenation reaction which is a part of the catabolic sequence of alpha-pinenes and beta-pinenes, limonene, DELTA1-p-methene and p-cymene
285942
Pseudomonas sp. PL-
perillyl aldehyde + NADH
285942
Pseudomonas sp. PL-
pH Optimum
7
NADH + perillyl aldehyde
Pseudomonas sp.
9.4
NAD+ + perillyl alcohol
Pseudomonas sp.
pH Range
5
8
pH 5: about 35% of activity maximum, pH 8: about 70% of activity maximum, NADH + perillyl aldehyde
Pseudomonas sp.
6.3
10.5
at pH 6.3 and 10.5: about 10% of activity maximum, perillyl alcohol + NAD+
Pseudomonas sp.
Cofactor
additional information
inactive with NADP+ and with thionicotinamide adenine dinucleotide
Pseudomonas sp.
NAD+
also active with some NAD+ analogues, e.g.: 3-acetylpyridine adenine dinucleotide, 3-pyridinealdehyde adenine dinucleotide, deamino-NAD
Pseudomonas sp.
Activating Compound (protein specific)
additional information
dehydrogenase is induced by limonene, alpha-pinene, delta-p-menthene and to lesser extend by p-cymene
Pseudomonas sp.
Cofactor (protein specific)
additional information
inactive with NADP+ and with thionicotinamide adenine dinucleotide
Pseudomonas sp.
NAD+
also active with some NAD+ analogues, e.g.: 3-acetylpyridine adenine dinucleotide, 3-pyridinealdehyde adenine dinucleotide, deamino-NAD
Pseudomonas sp.
Inhibitors (protein specific)
Ag+
0.005 mM, complete inhibition, prevented by 10 mM glutathione
Pseudomonas sp.
Cu2+
5 mM, complete inhibition, prevented by 5 mM glutathione
Pseudomonas sp.
Hg2+
0.005 mM, complete inhibition, prevented by 10 mM glutathione
Pseudomonas sp.
iodoacetate
10 mM, 95% inhibition, prevented by 10 mM glutathione
Pseudomonas sp.
additional information
glucose represses the induction of the enzyme by limonene
Pseudomonas sp.
NADH
competitive inhibition
Pseudomonas sp.
Ni2+
2 mM, 75% inhibition, complete reversion with 5 mM EDTA
Pseudomonas sp.
o-phenanthroline
at concentrations higher than 3 mM and low concentration of NAD+, about 50% inhibition
Pseudomonas sp.
p-hydroxymercuribenzoate
0.005 mM, complete inhibition, prevented by 10 mM glutathione
Pseudomonas sp.
Zn2+
2 mM, 75% inhibition, complete reversion with 10 mM EDTA
Pseudomonas sp.
KM Value [mM] (protein specific)
additional information
additional information
Pseudomonas sp.
0.077
NAD+
at pH 8.6
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
perillyl alcohol + NAD+
Pseudomonas sp.
catalyzes dehydrogenation reaction which is a part of the catabolic sequence of alpha-pinenes and beta-pinenes, limonene, DELTA1-p-methene and p-cymene
perillyl aldehyde + NADH
Pseudomonas sp.
perillyl alcohol + NAD+
Pseudomonas sp. PL-
catalyzes dehydrogenation reaction which is a part of the catabolic sequence of alpha-pinenes and beta-pinenes, limonene, DELTA1-p-methene and p-cymene
perillyl aldehyde + NADH
Pseudomonas sp. PL-
Purification (Commentary) (protein specific)
PL-strain
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
13
DEAE-eluate
Pseudomonas sp.
Storage Stability (protein specific)
-20°C, for at least 3 days, DEAE eluate
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
additional information
no substrates are p-menthan-7-ol, DELTA1-p-menthen-10-ol, DELTA8-menthen-10-ol, p-menthan-10-ol, myrtenol, isoborneol, borneol, cyclohexenol, o-menthyl benzyl alcohol, phthalyl alcohol, salicylic alcohol, 7-methyl cumic alcohol, ethanol, methanol
285942
Pseudomonas sp.
?
additional information
no substrates are p-menthan-7-ol, DELTA1-p-menthen-10-ol, DELTA8-menthen-10-ol, p-menthan-10-ol, myrtenol, isoborneol, borneol, cyclohexenol, o-menthyl benzyl alcohol, phthalyl alcohol, salicylic alcohol, 7-methyl cumic alcohol, ethanol, methanol
285942
Pseudomonas sp. PL-
?
perillyl alcohol + NAD+
e.g.: 8-hydroxyphellandrol, cumic alcohol
285942
Pseudomonas sp.
perillyl aldehyde + NADH
285942
Pseudomonas sp.
r
perillyl alcohol + NAD+
p-ethyl benzyl alcohol, p-methyl benzyl alcohol
285942
Pseudomonas sp.
perillyl aldehyde + NADH
285942
Pseudomonas sp.
r
perillyl alcohol + NAD+
rate of the reverse reaction is about 20% of the rate of the forward reaction, oxidizes a number of primary alcohols with the alcohol group allylic to an endocyclic double bond and a 6-membered ring, either aromatic or hydroaromatic
285942
Pseudomonas sp.
perillyl aldehyde + NADH
285942
Pseudomonas sp.
r
perillyl alcohol + NAD+
catalyzes dehydrogenation reaction which is a part of the catabolic sequence of alpha-pinenes and beta-pinenes, limonene, DELTA1-p-methene and p-cymene
285942
Pseudomonas sp.
perillyl aldehyde + NADH
285942
Pseudomonas sp.
perillyl alcohol + NAD+
e.g.: 8-hydroxyphellandrol, cumic alcohol
285942
Pseudomonas sp. PL-
perillyl aldehyde + NADH
285942
Pseudomonas sp. PL-
r
perillyl alcohol + NAD+
p-ethyl benzyl alcohol, p-methyl benzyl alcohol
285942
Pseudomonas sp. PL-
perillyl aldehyde + NADH
285942
Pseudomonas sp. PL-
r
perillyl alcohol + NAD+
rate of the reverse reaction is about 20% of the rate of the forward reaction, oxidizes a number of primary alcohols with the alcohol group allylic to an endocyclic double bond and a 6-membered ring, either aromatic or hydroaromatic
285942
Pseudomonas sp. PL-
perillyl aldehyde + NADH
285942
Pseudomonas sp. PL-
r
perillyl alcohol + NAD+
catalyzes dehydrogenation reaction which is a part of the catabolic sequence of alpha-pinenes and beta-pinenes, limonene, DELTA1-p-methene and p-cymene
285942
Pseudomonas sp. PL-
perillyl aldehyde + NADH
285942
Pseudomonas sp. PL-
pH Optimum (protein specific)
7
NADH + perillyl aldehyde
Pseudomonas sp.
9.4
NAD+ + perillyl alcohol
Pseudomonas sp.
pH Range (protein specific)
5
8
pH 5: about 35% of activity maximum, pH 8: about 70% of activity maximum, NADH + perillyl aldehyde
Pseudomonas sp.
6.3
10.5
at pH 6.3 and 10.5: about 10% of activity maximum, perillyl alcohol + NAD+
Pseudomonas sp.
Other publictions for EC 1.1.1.144
695811
Hoellrigl
TADH, the thermostable alcohol ...
Thermus sp., Thermus sp. ATN1
Appl. Microbiol. Biotechnol.
81
263-273
2008
-
-
-
-
-
-
-
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2
-
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2
-
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1
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1
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2
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285944
Gillooly
-
Production of a perillyl alcoh ...
Acinetobacter calcoaceticus
Biotechnol. Lett.
20
325-327
1998
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1
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1
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1
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1
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1
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1
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1
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1
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1
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285942
Ballal
Microbiological transformation ...
Pseudomonas sp., Pseudomonas sp. PL-
Indian J. Biochem.
5
1-6
1968
1
-
-
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10
2
-
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2
-
2
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1
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1
1
10
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2
2
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3
-
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1
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3
-
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10
-
2
-
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2
-
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-
1
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1
1
10
-
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-
2
2
-
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-
-
-
-
-
285943
Ballal
Perillyl alcohol dehydrogenase ...
Pseudomonas sp.
Biochem. Biophys. Res. Commun.
23
473-478
1966
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