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show all sequences of 1.1.1.143

Enzymic epimerization of sequoyitol to D-pinitol in Trifolium incarnatum

Ruis, H.; Hoffmann-Ostendorf, O.; Eur. J. Biochem. 7, 442-448 (1969)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
DTT
5fold activation
Trifolium incarnatum
GSH
5fold activation
Trifolium incarnatum
L-cysteine
5fold activation at 1.3 mM
Trifolium incarnatum
General Stability
General Stability
Organism
very unstable
Trifolium incarnatum
Inhibitors
Inhibitors
Commentary
Organism
Structure
iodoacetate
85% inhibition at 1 mM
Trifolium incarnatum
p-chloromercuribenzoate
complete inhibition at 1 mM
Trifolium incarnatum
Phenylmercurinitrate
complete inhibition at 1 mM
Trifolium incarnatum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
gel filtration
Trifolium incarnatum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5-O-methyl-myo-inositol + NAD+
Trifolium incarnatum
B-specificity
5D-5-O-methyl-2,3,5/4,6-pentahydroxycyclohexanone + NADH
-
Trifolium incarnatum
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Trifolium incarnatum
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Trifolium incarnatum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
plant
prior to the formation of flowers
Trifolium incarnatum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,4,6/3,5-pentahydroxycyclohexanone + NADH
-
285940
Trifolium incarnatum
? + NAD+
-
285940
Trifolium incarnatum
?
5-O-methyl-myo-inositol + NAD+
B-specificity
285940
Trifolium incarnatum
5D-5-O-methyl-2,3,5/4,6-pentahydroxycyclohexanone + NADH
-
285940
Trifolium incarnatum
r
DL-2,3,4,6/5-pentahydroxycyclohexanone + NADH
low activity
285940
Trifolium incarnatum
? + NAD+
-
285940
Trifolium incarnatum
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
9
-
Trifolium incarnatum
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Trifolium incarnatum
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
DTT
5fold activation
Trifolium incarnatum
GSH
5fold activation
Trifolium incarnatum
L-cysteine
5fold activation at 1.3 mM
Trifolium incarnatum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Trifolium incarnatum
General Stability (protein specific)
General Stability
Organism
very unstable
Trifolium incarnatum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
iodoacetate
85% inhibition at 1 mM
Trifolium incarnatum
p-chloromercuribenzoate
complete inhibition at 1 mM
Trifolium incarnatum
Phenylmercurinitrate
complete inhibition at 1 mM
Trifolium incarnatum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
gel filtration
Trifolium incarnatum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5-O-methyl-myo-inositol + NAD+
Trifolium incarnatum
B-specificity
5D-5-O-methyl-2,3,5/4,6-pentahydroxycyclohexanone + NADH
-
Trifolium incarnatum
r
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Trifolium incarnatum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
plant
prior to the formation of flowers
Trifolium incarnatum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,4,6/3,5-pentahydroxycyclohexanone + NADH
-
285940
Trifolium incarnatum
? + NAD+
-
285940
Trifolium incarnatum
?
5-O-methyl-myo-inositol + NAD+
B-specificity
285940
Trifolium incarnatum
5D-5-O-methyl-2,3,5/4,6-pentahydroxycyclohexanone + NADH
-
285940
Trifolium incarnatum
r
DL-2,3,4,6/5-pentahydroxycyclohexanone + NADH
low activity
285940
Trifolium incarnatum
? + NAD+
-
285940
Trifolium incarnatum
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
9
-
Trifolium incarnatum
Other publictions for EC 1.1.1.143
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
285940
Ruis
Enzymic epimerization of sequo ...
Trifolium incarnatum
Eur. J. Biochem.
7
442-448
1969
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