BRENDA - Enzyme Database show
show all sequences of 1.1.1.142

Enzymic epimerization of sequoyitol to D-pinitol in Trifolium incarnatum

Ruis, H.; Hoffmann-Ostendorf, O.; Eur. J. Biochem. 7, 442-448 (1969)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
21% inhibition at 0.1 mM
Trifolium incarnatum
Fe2+
25% inhibition at 0.1 mM
Trifolium incarnatum
p-chloromercuribenzoate
25% inhibition at 1 mM
Trifolium incarnatum
Phenylmercurinitrate
15% inhibition at 0.1 mM
Trifolium incarnatum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
gel filtration
Trifolium incarnatum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5D-5-O-methyl-chiro-inositol + NADP+
Trifolium incarnatum
A-site specificity
5D-5-O-methyl-2,3,5/4,6-pentahydroxycyclohexanone + NADPH + H+
-
Trifolium incarnatum
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Trifolium incarnatum
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Trifolium incarnatum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
plant
prior to formation of flowers
Trifolium incarnatum
-
Storage Stability
Storage Stability
Organism
2C, 0.01 M phosphate buffer, pH 7.2, little loss of activity within 3 weeks
Trifolium incarnatum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,4,6/3,5-pentahydroxycyclohexanone + NADPH
-
285940
Trifolium incarnatum
? + NADP+
-
285940
Trifolium incarnatum
?
5D-5-O-methyl-chiro-inositol + NADP+
A-site specificity
285940
Trifolium incarnatum
5D-5-O-methyl-2,3,5/4,6-pentahydroxycyclohexanone + NADPH + H+
-
285940
Trifolium incarnatum
r
D-2,3,5/4,6-pentahydroxycyclohexanone + NADPH
-
285940
Trifolium incarnatum
? + NADP+
-
285940
Trifolium incarnatum
?
dihydroxyacetone + NADPH
low activity
285940
Trifolium incarnatum
propanetriol + NADP+
-
285940
Trifolium incarnatum
?
DL-2,3,4,6/5-pentahydroxycyclohexanone + NADPH
low activity
285940
Trifolium incarnatum
? + NADP+
-
285940
Trifolium incarnatum
?
L-2,3,5/4,6-pentahydroxycyclohexanone + NADPH
low activity
285940
Trifolium incarnatum
? + NADP+
-
285940
Trifolium incarnatum
?
L-3-O-methyl-2,3,5/4,6-pentahydroxycyclohexanone + NADPH
low activity
285940
Trifolium incarnatum
? + NADP+
-
285940
Trifolium incarnatum
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
9
-
Trifolium incarnatum
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Trifolium incarnatum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Trifolium incarnatum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
21% inhibition at 0.1 mM
Trifolium incarnatum
Fe2+
25% inhibition at 0.1 mM
Trifolium incarnatum
p-chloromercuribenzoate
25% inhibition at 1 mM
Trifolium incarnatum
Phenylmercurinitrate
15% inhibition at 0.1 mM
Trifolium incarnatum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
gel filtration
Trifolium incarnatum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5D-5-O-methyl-chiro-inositol + NADP+
Trifolium incarnatum
A-site specificity
5D-5-O-methyl-2,3,5/4,6-pentahydroxycyclohexanone + NADPH + H+
-
Trifolium incarnatum
r
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Trifolium incarnatum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
plant
prior to formation of flowers
Trifolium incarnatum
-
Storage Stability (protein specific)
Storage Stability
Organism
2C, 0.01 M phosphate buffer, pH 7.2, little loss of activity within 3 weeks
Trifolium incarnatum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,4,6/3,5-pentahydroxycyclohexanone + NADPH
-
285940
Trifolium incarnatum
? + NADP+
-
285940
Trifolium incarnatum
?
5D-5-O-methyl-chiro-inositol + NADP+
A-site specificity
285940
Trifolium incarnatum
5D-5-O-methyl-2,3,5/4,6-pentahydroxycyclohexanone + NADPH + H+
-
285940
Trifolium incarnatum
r
D-2,3,5/4,6-pentahydroxycyclohexanone + NADPH
-
285940
Trifolium incarnatum
? + NADP+
-
285940
Trifolium incarnatum
?
dihydroxyacetone + NADPH
low activity
285940
Trifolium incarnatum
propanetriol + NADP+
-
285940
Trifolium incarnatum
?
DL-2,3,4,6/5-pentahydroxycyclohexanone + NADPH
low activity
285940
Trifolium incarnatum
? + NADP+
-
285940
Trifolium incarnatum
?
L-2,3,5/4,6-pentahydroxycyclohexanone + NADPH
low activity
285940
Trifolium incarnatum
? + NADP+
-
285940
Trifolium incarnatum
?
L-3-O-methyl-2,3,5/4,6-pentahydroxycyclohexanone + NADPH
low activity
285940
Trifolium incarnatum
? + NADP+
-
285940
Trifolium incarnatum
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
9
-
Trifolium incarnatum
Other publictions for EC 1.1.1.142
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
285940
Ruis
Enzymic epimerization of sequo ...
Trifolium incarnatum
Eur. J. Biochem.
7
442-448
1969
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4
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1
1
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1
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1
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1
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1
7
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1
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1
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1
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4
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1
1
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1
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1
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1
7
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1
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