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Literature summary for 1.1.1.141 extracted from

  • Parent, M.; Madore, E.; MacLaren, L.A.; Fortier, M.A.
    15-Hydroxyprostaglandin dehydrogenase in the bovine endometrium during the oestrous cycle and early pregnancy (2006), Reproduction, 131, 573-582.
    View publication on PubMed
Search for more literature for 1.1.1.141

Cloned(Commentary)

Cloned (Comment) Organism
15-PGDH, DNA and amino acid sequence determination and analysis, functional overexpression of His-tagged lung enzyme in Escherichia coli Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ Bos taurus
-
 (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus Q309F3
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged lung enzyme from Escherichia coli by nickel affinity chromatography Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
glandular epithelium
-
 Bos taurus
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lung
-
 Bos taurus
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uterine endometrium the enzyme is expressed mainly in the glandular epithelium, enzyme activity during the oestrous cycle and early pregnancy, overview Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 enzyme activity in the endometrium during the oestrous cycle and early pregnancy, overview Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+
-
 Bos taurus (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+
-
 ?
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ i.e. 15-hydroxyprostaglandin or (15S)-PGE2 Bos taurus (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
15-hydroxyprostaglandin dehydrogenase
-
 Bos taurus
15-PGDH
-
 Bos taurus
NAD+ dependent 15-hydroxyprostaglandin dehydrogenase
-
 Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 assay at Bos taurus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Bos taurus