Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ | Bos taurus | - |
(5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
native enzyme from lung by ammonium sulfate fractionation and acetone precipitation | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lung | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
purified enzyme | Bos taurus |
70 | - |
enzyme from lung | Bos taurus |
Storage Stability | Organism |
---|---|
-20°C | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ | - |
Bos taurus | (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+ | - |
? | |
(5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ | i.e. 15-hydroxyprostaglandin or (15S)-PGE2 | Bos taurus | (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+ | - |
? | |
11alpha,15(S)-dihydroxy-9-ketoprost-5,13-dienoic acid + NAD(P)+ | - |
Bos taurus | 11alpha-hydroxy-9,15-diketoprost-5,13-dienoic acid + NADH | - |
? | |
additional information | the enzyme catalyzes specifically the oxidation of the (15S)-hydroxy group of all natural prostaglandins except the B and 19-hydroxylated types | Bos taurus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
15-hydroxy prostaglandin dehydrogenase | - |
Bos taurus |
NAD+-15-hydroxy prostanoate oxidoreductase | - |
Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.7 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bos taurus |