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Literature summary for 1.1.1.140 extracted from

  • Alcantara, C.; Sarmiento-Rubiano, L.; Monedero, V.; Deutscher, J.; Perez-Martinez, G.; Yebra, M.
    Regulation of Lactobacillus casei sorbitol utilization genes requires DNA-binding transcriptional activator GutR and the conserved protein GutM (2008), Appl. Environ. Microbiol., 74, 5731-5740.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information gut genes are induced by sorbitol. GutR codes for a transcriptional activator of gut genes Lacticaseibacillus casei

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged GutR and GutM expressed in Escherichia coli M15(pREP4) Lacticaseibacillus casei

Protein Variants

Protein Variants Comment Organism
additional information mutant with a frameshift in gutR (strain BL285). The mutant loses the ability to ferment sorbitol, and transcription of the gut operon in cells grown with a mixture of ribose plus sorbitol is abolished. Sorbitol uptake rate in strain BL285 is 88fold lower than in the wild-type. GutB mutant (strain BL282) constitutively expresses the gut operon but is impaired in sorbitol uptake. GutM mutant (strain BL286) shows lower levels of gutFRMCBA transcription than the wild-type strain Lacticaseibacillus casei

Inhibitors

Inhibitors Comment Organism Structure
additional information gut genes are repressed by glucose Lacticaseibacillus casei

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane GutM Lacticaseibacillus casei 16020
-

Organism

Organism UniProt Comment Textmining
Lacticaseibacillus casei
-
ATCC 334 and BL23
-

Purification (Commentary)

Purification (Comment) Organism
GutR and GutM purified on Ni-NTA column Lacticaseibacillus casei

Storage Stability

Storage Stability Organism
-80°C, 10 mM NaH2PO4 buffer, pH 7.5, 45% glycerol, 10 mM beta-mercaptoethanol Lacticaseibacillus casei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-sorbitol 6-phosphate + NAD+
-
Lacticaseibacillus casei D-fructose 6-phosphate + NADH + H+
-
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Synonyms

Synonyms Comment Organism
GutF
-
Lacticaseibacillus casei
GutM
-
Lacticaseibacillus casei
GutR
-
Lacticaseibacillus casei
operon gutFRMCBA
-
Lacticaseibacillus casei
sorbitol-6-phosphate dehydrogenase
-
Lacticaseibacillus casei

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Lacticaseibacillus casei