BRENDA - Enzyme Database show
show all sequences of 1.1.1.140

High-level production of the low-calorie sugar sorbitol by Lactobacillus plantarum through metabolic engineering

Ladero, V.; Ramos, A.; Wiersma, A.; Goffin, P.; Schanck, A.; Kleerebezem, M.; Hugenholtz, J.; Smid, E.J.; Hols, P.; Appl. Environ. Microbiol. 73, 1864-1872 (2007)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
constitutive expression of the two sorbitol-6-phosphate dehydrogenase genes srlD1 and srlD2 in a mutant strain deficient for both L- and D-lactate dehydrogenase activities. Both Stl6PDH enzymes are active, and high specific activity can be detected in the overexpressing strains. Using resting cells under pH control with glucose as a substrate, both Stl6PDHs are capable of rerouting the glycolytic flux from fructose-6-phosphate toward sorbitol production with a remarkably high efficiency of 61 to 65% glucose conversion
Lactobacillus plantarum
Cloned(Commentary)
Commentary
Organism
constitutive expression in Lactobacillus plantarum
Lactobacillus plantarum
Engineering
Amino acid exchange
Commentary
Organism
additional information
constitutive expression of the two sorbitol-6-phosphate dehydrogenase genes srlD1 and srlD2 in a mutant strain deficient for both L- and D-lactate dehydrogenase activities. Both Stl6PDH enzymes are active, and high specific activity can be detected in the overexpressing strains. Using resting cells under pH control with glucose as a substrate, both Stl6PDHs are capable of rerouting the glycolytic flux from fructose-6-phosphate toward sorbitol production with a remarkably high efficiency of 61 to 65% glucose conversion
Lactobacillus plantarum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lactobacillus plantarum
-
-
-
Application (protein specific)
Application
Commentary
Organism
synthesis
constitutive expression of the two sorbitol-6-phosphate dehydrogenase genes srlD1 and srlD2 in a mutant strain deficient for both L- and D-lactate dehydrogenase activities. Both Stl6PDH enzymes are active, and high specific activity can be detected in the overexpressing strains. Using resting cells under pH control with glucose as a substrate, both Stl6PDHs are capable of rerouting the glycolytic flux from fructose-6-phosphate toward sorbitol production with a remarkably high efficiency of 61 to 65% glucose conversion
Lactobacillus plantarum
Cloned(Commentary) (protein specific)
Commentary
Organism
constitutive expression in Lactobacillus plantarum
Lactobacillus plantarum
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
constitutive expression of the two sorbitol-6-phosphate dehydrogenase genes srlD1 and srlD2 in a mutant strain deficient for both L- and D-lactate dehydrogenase activities. Both Stl6PDH enzymes are active, and high specific activity can be detected in the overexpressing strains. Using resting cells under pH control with glucose as a substrate, both Stl6PDHs are capable of rerouting the glycolytic flux from fructose-6-phosphate toward sorbitol production with a remarkably high efficiency of 61 to 65% glucose conversion
Lactobacillus plantarum
Other publictions for EC 1.1.1.140
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
726181
Liang
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Malus domestica
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904-914
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695750
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Lactobacillus casei
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1
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Metabolic engineering for biop ...
Lactobacillus plantarum
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684551
Ladero
High-level production of the l ...
Lactobacillus plantarum
Appl. Environ. Microbiol.
73
1864-1872
2007
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1
1
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1
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690053
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Expression and cloning of the ...
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406-412
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5
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669655
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Sorbitol synthesis by an engin ...
Lactobacillus casei
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285910
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Temperature-dependent fermenta ...
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285911
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Sorbitol-6-phosphate dehydroge ...
Nicotiana tabacum
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117
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1
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285903
Sadegh Roohi
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Regulation of sorbitol metabol ...
Clostridium pasteurianum
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2207-2215
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285907
Novotny
Purification and properties of ...
Escherichia coli, Escherichia coli LJ378
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159
986-990
1984
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285904
Dills
Regulation of hexitol cataboli ...
Streptococcus mutans
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285909
Hirai
Purification and characteristi ...
Eriobotrya japonica
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67
221-224
1981
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285908
Hirai
Sorbitol-6-phosphate dehydroge ...
Eriobotrya japonica
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63
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1979
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285905
Du Toit
The isolation and characteriza ...
Clostridium pasteurianum
Biochim. Biophys. Acta
206
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1970
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10
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285906
Horwitz
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D-mannitol 1-phosphate dehydro ...
Enterobacter aerogenes
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9
150-155
1966
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285902
Liss
D-Mannitol 1-phosphate dehydro ...
Enterobacter aerogenes
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237
1342-1350
1962
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4
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