BRENDA - Enzyme Database show
show all sequences of 1.1.1.140

Purification and properties of D-mannitol-1-phosphate dehydrogenase and D-glucitol-6-phosphate dehydrogenase from Escherichia coli

Novotny, M.J.; Reizer, J.; Esch, F.; Saier, M.H.; J. Bacteriol. 159, 986-990 (1984)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
cloning and subcloning of the gut operon and gutD gene
Escherichia coli
Inhibitors
Inhibitors
Commentary
Organism
Structure
diethyl dicarbonate
100% inactivation
Escherichia coli
N-ethylmaleimide
about 90% inactivation
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
NAD+
-
Escherichia coli
3.3
-
D-sorbitol 6-phosphate
-
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
26000
-
4 * 26000, SDS-PAGE
Escherichia coli
117000
-
gel filtration
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
strain LJ378
-
Escherichia coli LJ378
-
strain LJ378
-
Purification (Commentary)
Commentary
Organism
-
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
180
-
-
Escherichia coli
Storage Stability
Storage Stability
Organism
-20C, more than 4 months, no appreciable loss of activity
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-sorbitol 6-phosphate + NAD+
high specificity for NAD+
285907
Escherichia coli
D-fructose 6-phosphate + NADH + H+
-
-
-
?
D-sorbitol 6-phosphate + NAD+
high specificity for D-sorbitol 6-phosphate
285907
Escherichia coli
D-fructose 6-phosphate + NADH + H+
-
-
-
?
D-sorbitol 6-phosphate + NAD+
high specificity for NAD+
285907
Escherichia coli LJ378
D-fructose 6-phosphate + NADH + H+
-
-
-
?
D-sorbitol 6-phosphate + NAD+
high specificity for D-sorbitol 6-phosphate
285907
Escherichia coli LJ378
D-fructose 6-phosphate + NADH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 26000, SDS-PAGE
Escherichia coli
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
additional information
-
assay at room temperature
Escherichia coli
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
40
-
20 min, 85% loss of activity
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.7
-
-
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no activity with NADP+
Escherichia coli
NAD+
specific for
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
cloning and subcloning of the gut operon and gutD gene
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no activity with NADP+
Escherichia coli
NAD+
specific for
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
diethyl dicarbonate
100% inactivation
Escherichia coli
N-ethylmaleimide
about 90% inactivation
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
NAD+
-
Escherichia coli
3.3
-
D-sorbitol 6-phosphate
-
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
26000
-
4 * 26000, SDS-PAGE
Escherichia coli
117000
-
gel filtration
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
180
-
-
Escherichia coli
Storage Stability (protein specific)
Storage Stability
Organism
-20C, more than 4 months, no appreciable loss of activity
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-sorbitol 6-phosphate + NAD+
high specificity for NAD+
285907
Escherichia coli
D-fructose 6-phosphate + NADH + H+
-
-
-
?
D-sorbitol 6-phosphate + NAD+
high specificity for D-sorbitol 6-phosphate
285907
Escherichia coli
D-fructose 6-phosphate + NADH + H+
-
-
-
?
D-sorbitol 6-phosphate + NAD+
high specificity for NAD+
285907
Escherichia coli LJ378
D-fructose 6-phosphate + NADH + H+
-
-
-
?
D-sorbitol 6-phosphate + NAD+
high specificity for D-sorbitol 6-phosphate
285907
Escherichia coli LJ378
D-fructose 6-phosphate + NADH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 26000, SDS-PAGE
Escherichia coli
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
additional information
-
assay at room temperature
Escherichia coli
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
40
-
20 min, 85% loss of activity
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.7
-
-
Escherichia coli
Other publictions for EC 1.1.1.140
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
726181
Liang
-
Genomic structure, sub-cellula ...
Malus domestica
Plant Mol. Biol. Rep.
30
904-914
2012
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1
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695750
Alcantara
Regulation of Lactobacillus ca ...
Lactobacillus casei
Appl. Environ. Microbiol.
74
5731-5740
2008
1
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697515
Akinterinwa
Metabolic engineering for biop ...
Lactobacillus plantarum
Curr. Opin. Biotechnol.
19
461-467
2008
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684551
Ladero
High-level production of the l ...
Lactobacillus plantarum
Appl. Environ. Microbiol.
73
1864-1872
2007
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1
1
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1
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3
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688138
Duangsrisai
-
Presence and expression of NAD ...
Fragaria x ananassa
J. Hortic. Sci. Biotechnol.
82
191-198
2007
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1
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690053
Kim
-
Expression and cloning of the ...
Pyrus pyrifolia
Sci. Hortic.
112
406-412
2007
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1
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5
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1
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1
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1
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1
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1
5
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1
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5
5
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1
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1
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1
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1
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669655
Roux
Preliminary studies on the inh ...
Escherichia coli
J. Enzyme Inhib. Med. Chem.
21
187-192
2006
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1
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3
2
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1
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1
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1
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668664
Nissen
Sorbitol synthesis by an engin ...
Lactobacillus casei
FEMS Microbiol. Lett.
249
177-183
2005
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1
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1
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3
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1
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2
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657114
Kanamaru
Transgenic apple transformed b ...
Malus domestica
Plant Sci.
167
55-61
2004
-
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1
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1
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1
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1
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1
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2
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285910
Bouvet
Temperature-dependent fermenta ...
Escherichia coli
Appl. Environ. Microbiol.
65
4245-4247
1999
-
-
-
-
-
-
-
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3
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1
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1
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285911
Sheveleva
Sorbitol-6-phosphate dehydroge ...
Nicotiana tabacum
Plant Physiol.
117
831-839
1998
-
-
1
-
-
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-
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4
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285903
Sadegh Roohi
-
Regulation of sorbitol metabol ...
Clostridium pasteurianum
J. Gen. Microbiol.
133
2207-2215
1987
-
-
-
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285907
Novotny
Purification and properties of ...
Escherichia coli, Escherichia coli LJ378
J. Bacteriol.
159
986-990
1984
-
-
1
-
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2
2
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2
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3
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1
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1
1
4
1
1
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1
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1
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2
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1
2
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2
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2
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2
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1
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1
1
4
1
1
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1
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1
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285904
Dills
Regulation of hexitol cataboli ...
Streptococcus mutans
J. Bacteriol.
153
861-866
1983
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285909
Hirai
Purification and characteristi ...
Eriobotrya japonica
Plant Physiol.
67
221-224
1981
-
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9
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285908
Hirai
Sorbitol-6-phosphate dehydroge ...
Eriobotrya japonica
Plant Physiol.
63
715-717
1979
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285905
Du Toit
The isolation and characteriza ...
Clostridium pasteurianum
Biochim. Biophys. Acta
206
333-342
1970
-
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2
10
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1
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2
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1
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4
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1
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3
1
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3
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3
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2
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10
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1
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4
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1
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3
1
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285906
Horwitz
-
D-mannitol 1-phosphate dehydro ...
Enterobacter aerogenes
Methods Enzymol.
9
150-155
1966
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1
3
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1
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1
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1
3
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1
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285902
Liss
D-Mannitol 1-phosphate dehydro ...
Enterobacter aerogenes
J. Biol. Chem.
237
1342-1350
1962
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1
4
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1
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4
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1
1
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