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Literature summary for 1.1.1.14 extracted from

  • Yamaguchi, H.; Kanayama, Y.; Yamaki, S.
    Purification and properties of NAD-dependent sorbitol dehydrogenase from apple fruit (1994), Plant Cell Physiol., 35, 887-892.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
CaCl2
-
Malus domestica
HgCl2
-
Malus domestica
MgCl2
-
Malus domestica
ZnCl2
-
Malus domestica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
40.3
-
D-sorbitol
-
Malus domestica
215
-
D-fructose
-
Malus domestica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
gel filtration Malus domestica

Organism

Organism UniProt Comment Textmining
Malus domestica
-
apple
-
Malus domestica
-
cv. Ourin
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
Malus domestica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.105
-
-
Malus domestica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose + NADH + H+
-
Malus domestica D-sorbitol + NAD+
-
?
D-mannitol + NAD+
-
Malus domestica ?
-
?
D-ribitol + NAD+
-
Malus domestica ?
-
?
D-sorbitol + NAD+
-
Malus domestica D-fructose + NADH + H+
-
?
D-xylitol + NAD+
-
Malus domestica ?
-
?
ethanol + NAD+
-
Malus domestica acetaldehyde + NADH
-
?
L-arabitol + NAD+ low activity Malus domestica ?
-
?
L-threitol + NAD+
-
Malus domestica ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
D-fructose reduction Malus domestica
9.6
-
sorbitol oxidation Malus domestica

pH Range

pH Minimum pH Maximum Comment Organism
5 7 30% of maximal activity at pH 5.0 and 60% of maximal activity at pH 7.0, fructose reduction Malus domestica
9 10.5 less than 70% of maximal activity above and below, sorbitol oxidation Malus domestica

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Malus domestica
NADH
-
Malus domestica