Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.14 extracted from

  • Bailey, J.P.; Renz, C.; McGuinness, E.T.
    Sorbitol dehydrogenase from horse liver: Purification, characterization and comparative properties (1981), Comp. Biochem. Physiol. B, 69, 909-914.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
NAD+ above 7 mM, substrate inhibition Equus caballus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
NADH
-
Equus caballus
0.041
-
NAD+
-
Equus caballus
0.24
-
D-sorbitol
-
Equus caballus
330
-
D-fructose
-
Equus caballus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
x * 26000, SDS-PAGE Ovis aries
26000
-
x * 26000, SDS-PAGE Equus caballus
106000
-
gel filtration Ovis aries
110000
-
gel filtration Equus caballus

Organism

Organism UniProt Comment Textmining
Equus caballus
-
-
-
Ovis aries
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Equus caballus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Ovis aries
-
liver
-
Equus caballus
-

Storage Stability

Storage Stability Organism
-20°C, stable for 4 months Equus caballus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose + NADH + H+
-
Ovis aries D-sorbitol + NAD+
-
?
D-fructose + NADH + H+
-
Equus caballus D-sorbitol + NAD+
-
?
D-galactitol + NAD+ low activity Equus caballus D-tagatose + NADH
-
?
D-mannitol + NAD+
-
Ovis aries ?
-
?
D-mannitol + NAD+
-
Equus caballus ?
-
?
D-ribitol + NAD+
-
Ovis aries ?
-
?
D-ribitol + NAD+
-
Equus caballus ?
-
?
D-sorbitol + NAD+
-
Ovis aries D-fructose + NADH + H+
-
?
D-sorbitol + NAD+
-
Equus caballus D-fructose + NADH + H+
-
?
D-xylitol + NAD+
-
Ovis aries ?
-
?
D-xylitol + NAD+
-
Equus caballus ?
-
?
L-arabitol + NAD+
-
Ovis aries ?
-
?
L-arabitol + NAD+ low activity Equus caballus ?
-
?
L-iditol + NAD+
-
Ovis aries ?
-
?
L-iditol + NAD+
-
Equus caballus ?
-
?
L-sorbose + NADH
-
Equus caballus ?
-
?

Subunits

Subunits Comment Organism
? x * 26000, SDS-PAGE Ovis aries
? x * 26000, SDS-PAGE Equus caballus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
30% loss of activity after 3 days, 70% loss of activity after 6 days, 100% loss of activity after 10 days Equus caballus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
D-fructose reduction Equus caballus
9.6
-
sorbitol oxidation Equus caballus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Ovis aries
NAD+ specific for Equus caballus
NADH
-
Ovis aries
NADH specific for Equus caballus