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Literature summary for 1.1.1.14 extracted from

  • Negm, F.B.; Loescher, W.H.
    Detection and characterization of sorbitol dehydrogenase from apple callus tissue (1979), Plant Physiol., 64, 69-73.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
ZnSO4 reverses cysteine inhibition Malus domestica

Inhibitors

Inhibitors Comment Organism Structure
AgNO3
-
Malus domestica
cysteine strong effect on sorbitol oxidation, slight effect on fructose reduction, ZnSO4 reverses inhibition Malus domestica
HgCl2
-
Malus domestica
iodoacetate strong effect on sorbitol oxidation, weaker effect on fructose reduction Malus domestica
p-chloromercuribenzoate
-
Malus domestica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
37
-
D-xylitol
-
Malus domestica
86
-
D-sorbitol
-
Malus domestica
1500
-
D-fructose
-
Malus domestica

Organism

Organism UniProt Comment Textmining
Malus domestica
-
apple
-
Malus domestica
-
cv Granny Smith
-

Source Tissue

Source Tissue Comment Organism Textmining
mesocarp tissue culture Malus domestica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose + NADH + H+
-
Malus domestica D-sorbitol + NAD+
-
?
D-mannitol + NAD+
-
Malus domestica ?
-
?
D-ribitol + NAD+
-
Malus domestica ?
-
?
D-sorbitol + NAD+
-
Malus domestica D-fructose + NADH + H+
-
?
D-xylitol + NAD+
-
Malus domestica ?
-
?
L-arabitol + NAD+
-
Malus domestica ?
-
?
L-sorbose + NADH
-
Malus domestica ?
-
?
L-threitol + NAD+
-
Malus domestica ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Malus domestica

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 65 35°C: about 50% of activity maximum, 65°C: about 95% of activity maximum Malus domestica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.7 7 D-fructose reduction Malus domestica
9 10 sorbitol oxidation Malus domestica

pH Range

pH Minimum pH Maximum Comment Organism
4.7 8 at pH 4.7 and 8 about 50% of activity maximum, D-fructose reduction Malus domestica
8 11 at pH 8 and 11 about 50% of activity maximum, sorbitol oxidation Malus domestica

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5
-
at lower pH denaturation occurs Malus domestica

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Malus domestica
NADH
-
Malus domestica
NADP+ cannot replace NAD+ Malus domestica
NADPH less effective than NADH Malus domestica