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Literature summary for 1.1.1.14 extracted from

  • Leissing, N.; McGuinness, E.T.
    Sorbitol dehydrogenase from rat liver (1982), Methods Enzymol., 89, 135-140.
    View publication on PubMed

General Stability

General Stability Organism
0.1 mg/ml bovine serum albumin stabilizes at 4°C Rattus norvegicus
278 mM maltose stabilizes at 4°C Rattus norvegicus
6.8 M glycerol stabilizes at 4°C Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
D-fructose above 250 mM, substrate inhibition Rattus norvegicus
D-sorbitol above 49 mM, substrate inhibition Rattus norvegicus
NAD+ above 7 mM, substrate inhibition Rattus norvegicus
NADH above 0.4 mM, substrate inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.067
-
NADH
-
Rattus norvegicus
0.082
-
NAD+
-
Rattus norvegicus
0.38
-
D-sorbitol
-
Rattus norvegicus
136
-
D-fructose
-
Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
95000
-
gel filtration Rattus norvegicus
97000
-
disc gel electrophoresis Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Ovis aries
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-
liver
-
Ovis aries
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.47
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, 70% loss of activity after 1 week, 0.1 mg/ml bovine serum albumin stabilizes Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-arabitol + NAD+ D-arabinitol is identical, low activity Rattus norvegicus ?
-
?
D-arabitol + NAD+ D-arabinitol is identical, low activity Ovis aries ?
-
?
D-fructose + NADH + H+
-
Rattus norvegicus D-sorbitol + NAD+
-
?
D-fructose + NADH + H+
-
Ovis aries D-sorbitol + NAD+
-
?
D-mannitol + NAD+
-
Rattus norvegicus ?
-
?
D-mannitol + NAD+
-
Ovis aries ?
-
?
D-ribitol + NAD+
-
Rattus norvegicus ?
-
?
D-ribitol + NAD+
-
Ovis aries ?
-
?
D-sorbitol + NAD+
-
Rattus norvegicus D-fructose + NADH + H+
-
?
D-sorbitol + NAD+
-
Ovis aries D-fructose + NADH + H+
-
?
D-xylitol + NAD+
-
Rattus norvegicus ?
-
?
D-xylitol + NAD+
-
Ovis aries ?
-
?
L-sorbose + NADH
-
Rattus norvegicus ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 35000-40000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
22
-
5 h, stable Rattus norvegicus
60
-
10 min, 65% loss of activity Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.9
-
D-fructose reduction Rattus norvegicus
8.1 8.5 sorbitol oxidation Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Ovis aries
NAD+ specific for Rattus norvegicus
NADH
-
Ovis aries
NADH specific for Rattus norvegicus