Cloned (Comment) | Organism |
---|---|
gene mdh, DNA and amino acid sequence determination and compiational amnipulation, expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Limosilactobacillus reuteri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 93% inhibition at 10 mM within 20 min, reversible by Zn2+ which restores 66% of control activity at 20 mM | Limosilactobacillus reuteri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
NADPH | pH 5.4, 37°C, recombinant enzyme | Limosilactobacillus reuteri | |
0.24 | - |
NADH | pH 5.4, 37°C, recombinant enzyme | Limosilactobacillus reuteri | |
0.35 | - |
NADP+ | pH 6.2, 37°C, recombinant enzyme | Limosilactobacillus reuteri | |
9 | - |
NAD+ | pH 6.2, 37°C, recombinant enzyme | Limosilactobacillus reuteri | |
34 | - |
D-fructose | pH 5.4, 37°C, recombinant enzyme | Limosilactobacillus reuteri | |
54 | - |
D-mannitol | pH 6.2, 37°C, recombinant enzyme | Limosilactobacillus reuteri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | content determination at 20°C, 0.87 mol Zn2+ per subunit | Limosilactobacillus reuteri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37279 | - |
2 * 40000, recombinant enzyme, SDS-PAGE, 2 * 37279, recombinant His6-tagged enzyme, mass spectrometry | Limosilactobacillus reuteri |
40000 | - |
2 * 40000, recombinant enzyme, SDS-PAGE, 2 * 37279, recombinant His6-tagged enzyme, mass spectrometry | Limosilactobacillus reuteri |
75000 | - |
recombinant enzyme, gel filtration | Limosilactobacillus reuteri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol + NAD(P)+ | Limosilactobacillus reuteri | - |
D-fructose + NAD(P)H + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Limosilactobacillus reuteri | - |
strain ATCC 53608, gene mdh | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultratfiltration | Limosilactobacillus reuteri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.61 | - |
crude recombinant Escherichia coli cell extract | Limosilactobacillus reuteri |
124.6 | - |
purified recombinant enzyme | Limosilactobacillus reuteri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol + NAD(P)+ | - |
Limosilactobacillus reuteri | D-fructose + NAD(P)H + H+ | - |
r | |
D-mannitol + NAD+ | - |
Limosilactobacillus reuteri | D-fructose + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000, recombinant enzyme, SDS-PAGE, 2 * 37279, recombinant His6-tagged enzyme, mass spectrometry | Limosilactobacillus reuteri |
Synonyms | Comment | Organism |
---|---|---|
LRMDH | - |
Limosilactobacillus reuteri |
mannitol dehydrogenase | - |
Limosilactobacillus reuteri |
MDH | - |
Limosilactobacillus reuteri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
recombinant enzyme | Limosilactobacillus reuteri |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified recombinant enzyme, 5 min, complete inactivation | Limosilactobacillus reuteri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.4 | - |
reverse reaction, recombinant enzyme | Limosilactobacillus reuteri |
6.2 | - |
forward reaction, recombinant enzyme | Limosilactobacillus reuteri |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | reverse reaction, 81% of maximal activity at pH 5.0, 22% at pH 7.0, 72% at pH 5.6, and 60% at pH 7.4 | Limosilactobacillus reuteri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)+ | NADP+ is the preferred cofactor in the forward reaction | Limosilactobacillus reuteri | |
NAD(P)H | NADPH is the preferred cofactor in the reverse reaction | Limosilactobacillus reuteri |