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Literature summary for 1.1.1.138 extracted from

  • Morton, N.; Dickerson, A.G.; Hammond, J.B.W.
    Mannitol metabolism in Agaricus bisporus: Purification and properties of mannitol dehydrogenase (1985), J. Gen. Microbiol., 131, 2885-2890.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
NADP+ D-fructose reduction, NADP+/NADPH ratio of 38 necessary for 50% inhibition Agaricus bisporus
NADPH D-mannitol oxidation, NADPH/NADP+ ratio of 0.1 necessary for 50% inhibition Agaricus bisporus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.036
-
NADP+ pH 7.0 Agaricus bisporus
0.0385
-
NADPH pH 7.0 Agaricus bisporus
0.082
-
NADP+ pH 10.25 Agaricus bisporus
16.2
-
D-mannitol pH 10.25 and pH 7.0 Agaricus bisporus
1200
-
D-fructose at pH 7.0 Agaricus bisporus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29500
-
4 * 29500, SDS-PAGE Agaricus bisporus
115000 130000 gel filtration, rate zonal ultracentrifugation Agaricus bisporus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-fructose + NADPH Agaricus bisporus
-
D-mannitol + NADP+
-
r
D-mannitol + NADP+ Agaricus bisporus
-
D-fructose + NADPH + H+
-
r

Organism

Organism UniProt Comment Textmining
Agaricus bisporus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Agaricus bisporus

Source Tissue

Source Tissue Comment Organism Textmining
fruit body
-
Agaricus bisporus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
194.4
-
-
Agaricus bisporus

Storage Stability

Storage Stability Organism
-20°C, 10 mM Tris-HCl, pH 7.5, 10 mM 2-mercaptoethanol, 0.05 mM PMSF, 50%, v/v, glycerol, 1 month Agaricus bisporus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose + NADPH
-
Agaricus bisporus D-mannitol + NADP+
-
r
D-mannitol + NADP+
-
Agaricus bisporus D-fructose + NADPH + H+
-
r
L-sorbitol + NADP+ 7.7% of the activity compared to D-mannitol Agaricus bisporus L-sorbose + NADPH
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 29500, SDS-PAGE Agaricus bisporus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
D-fructose reduction Agaricus bisporus
10.25
-
D-mannitol oxidation Agaricus bisporus

Cofactor

Cofactor Comment Organism Structure
NADP+ cannot be replaced by NAD+ Agaricus bisporus
NADPH cannot be replaced by NADH Agaricus bisporus