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show all sequences of 1.1.1.136

Structural and genetic characterization of the Escherichia coli O180 O antigen and identification of a UDP-GlcNAc 6-dehydrogenase

Wang, Q.; Perepelov, A.V.; Beutin, L.; Senchenkova, S.N.; Xu, Y.; Shashkov, A.S.; Ding, P.; Knirel, Y.A.; Feng, L.; Glycobiology 22, 1321-1331 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.84
-
UDP-N-acetyl-D-glucosamine
pH 8, 37°C
Escherichia coli
4.16
-
NAD+
pH 8, 37°C
Escherichia coli
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
inhibition
Escherichia coli
Fe3+
inhibition
Escherichia coli
K+
enhancing activity
Escherichia coli
Mn2+
inhibition
Escherichia coli
NH4+
enhancing activity
Escherichia coli
Ni2+
inhibition
Escherichia coli
Zn2+
inhibition
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50500
-
calculated from cDNA
Escherichia coli
51500
-
SDS-PAGE, His-tagged gnaA
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-N-acetyl-D-glucosamine + 2 NAD+ + H2O
-
725107
Escherichia coli
UDP-N-acetyl-2-amino-2-deoxy-D-glucuronate + 2 NADH + 2 H+
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Escherichia coli
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
50
-
no activity above 50°C
Escherichia coli
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.97
-
UDP-N-acetyl-D-glucosamine
pH 8, 37°C
Escherichia coli
1.41
-
NAD+
pH 8, 37°C
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.84
-
UDP-N-acetyl-D-glucosamine
pH 8, 37°C
Escherichia coli
4.16
-
NAD+
pH 8, 37°C
Escherichia coli
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
inhibition
Escherichia coli
Fe3+
inhibition
Escherichia coli
K+
enhancing activity
Escherichia coli
Mn2+
inhibition
Escherichia coli
NH4+
enhancing activity
Escherichia coli
Ni2+
inhibition
Escherichia coli
Zn2+
inhibition
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50500
-
calculated from cDNA
Escherichia coli
51500
-
SDS-PAGE, His-tagged gnaA
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-N-acetyl-D-glucosamine + 2 NAD+ + H2O
-
725107
Escherichia coli
UDP-N-acetyl-2-amino-2-deoxy-D-glucuronate + 2 NADH + 2 H+
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Escherichia coli
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
50
-
no activity above 50°C
Escherichia coli
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.97
-
UDP-N-acetyl-D-glucosamine
pH 8, 37°C
Escherichia coli
1.41
-
NAD+
pH 8, 37°C
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Escherichia coli
Other publictions for EC 1.1.1.136
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725107
Wang
Structural and genetic charact ...
Escherichia coli
Glycobiology
22
1321-1331
2012
-
-
1
-
-
-
-
2
-
7
2
-
-
4
-
-
-
-
-
-
-
-
1
-
1
1
-
2
1
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-
-
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-
1
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-
-
-
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-
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2
-
7
2
-
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-
-
-
-
-
-
1
-
1
1
-
2
1
-
-
-
-
-
-
-
-
-
712456
Gu
Biosynthesis of a new UDP-suga ...
Bacillus cereus, Bacillus cereus subsp. cytotoxis, NVH 391-98
J. Biol. Chem.
285
24825-24833
2010
-
-
1
-
-
-
-
-
-
-
1
-
-
8
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
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-
667737
Zhang
Vi antigen biosynthesis in Sal ...
Salmonella enterica subsp. enterica serovar Typhi
Biochemistry
45
8163-8173
2006
-
-
1
-
-
-
-
2
-
1
2
1
-
3
-
-
1
-
-
-
1
1
3
1
1
-
-
2
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
1
2
1
-
-
-
1
-
-
1
1
3
1
1
-
-
2
1
1
-
-
-
-
-
-
-
-
656241
Miller
Biochemical characterization o ...
Pseudomonas aeruginosa
J. Biol. Chem.
279
37551-37558
2004
2
-
1
-
-
-
-
1
-
1
3
-
-
3
-
-
1
-
-
-
-
1
1
1
1
-
-
-
1
-
-
1
-
2
-
2
-
1
1
-
-
-
-
-
-
1
-
1
3
-
-
-
-
1
-
-
-
1
1
1
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2273
Kawamura
Biosynthesis of UDP-N-acetyl-D ...
Micrococcus luteus
J. Biochem.
98
105-116
1985
1
-
-
-
-
2
3
2
-
-
-
1
-
3
-
-
1
-
-
-
1
1
1
-
-
-
-
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2
-
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1
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1
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1
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2
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3
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2
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1
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1
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1
1
1
-
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2
-
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-
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-
-
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-
285850
Wheat
Cellular localization of uridi ...
Citrobacter freundii
J. Bacteriol.
127
1032-1035
1976
-
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1
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2
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1
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1
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-
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285848
Fan
UDPacetylglucosamine dehydroge ...
Alcaligenes sp.
Arch. Biochem. Biophys.
135
45-49
1969
-
-
-
-
-
-
1
2
-
-
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1
-
2
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-
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3
-
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1
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2
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2
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1
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2
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1
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3
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1
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