BRENDA - Enzyme Database show
show all sequences of 1.1.1.136

Vi antigen biosynthesis in Salmonella typhi: characterization of UDP-N-acetylglucosamine C-6 dehydrogenase (TviB) and UDP-N-acetylglucosaminuronic acid C-4 epimerase (TviC)

Zhang, H.; Zhou, Y.; Bao, H.; Liu, H.W.; Biochemistry 45, 8163-8173 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene tviB located in the viaB locus, genetic organization, expression as C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
Salmonella enterica subsp. enterica serovar Typhi
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.077
-
UDP-N-acetyl-D-glucosamine
pH 7.5, 25C, recombinant enzyme
Salmonella enterica subsp. enterica serovar Typhi
0.276
-
NAD+
pH 7.5, 25C, recombinant enzyme
Salmonella enterica subsp. enterica serovar Typhi
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Salmonella enterica subsp. enterica serovar Typhi
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
47675
-
3 * 47675, subunit amino acid sequence calculation
Salmonella enterica subsp. enterica serovar Typhi
120500
-
recombinant enzyme, gel filtration
Salmonella enterica subsp. enterica serovar Typhi
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-N-acetyl-D-glucosamine + NAD+ + H2O
Salmonella enterica subsp. enterica serovar Typhi
the enzyme is involved in the biosynthesis of the Vi antigen, a clinically important vaccine antigen, in Salmonella typhi, pathway overview
UDP-N-acetyl-2-amino-2-deoxy-D-glucuronate + NADH
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Salmonella enterica subsp. enterica serovar Typhi
-
ATCC 700931, gene tviB
-
Purification (Commentary)
Commentary
Organism
recombinant C-terminally His6-tagged TviB from Escherichia coli strain BL21(DE3) by two steps of anion exchange chromatography, affinity chromatography, and gel filtration, inactive oligomeric forms of the enzym eis removed in gel filtration, to near homogeneity
Salmonella enterica subsp. enterica serovar Typhi
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Salmonella enterica subsp. enterica serovar Typhi
Storage Stability
Storage Stability
Organism
-80C, purified recombinant enzyme, in presence of 10 mM DTT, stable, even to multiple freeze-thaw cycles
Salmonella enterica subsp. enterica serovar Typhi
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
determination of substrate specificity, no or poor activity with UDP-D-glucose, UDP-D-galactose, and UDP-D-galactosamine, overview
667737
Salmonella enterica subsp. enterica serovar Typhi
?
-
-
-
-
UDP-N-acetyl-D-glucosamine + NAD+ + H2O
-
667737
Salmonella enterica subsp. enterica serovar Typhi
UDP-N-acetyl-2-amino-2-deoxy-D-glucuronate + NADH
product determination by NMR
-
-
?
UDP-N-acetyl-D-glucosamine + NAD+ + H2O
the enzyme is involved in the biosynthesis of the Vi antigen, a clinically important vaccine antigen, in Salmonella typhi, pathway overview
667737
Salmonella enterica subsp. enterica serovar Typhi
UDP-N-acetyl-2-amino-2-deoxy-D-glucuronate + NADH
-
-
-
?
Subunits
Subunits
Commentary
Organism
trimer
3 * 47675, subunit amino acid sequence calculation
Salmonella enterica subsp. enterica serovar Typhi
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
30
assay at
Salmonella enterica subsp. enterica serovar Typhi
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
15.5
-
NAD+
pH 7.5, 25C, recombinant enzyme
Salmonella enterica subsp. enterica serovar Typhi
15.5
-
UDP-N-acetyl-D-glucosamine
pH 7.5, 25C, recombinant enzyme
Salmonella enterica subsp. enterica serovar Typhi
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
-
Salmonella enterica subsp. enterica serovar Typhi
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
8.4
inactive above pH 8.4
Salmonella enterica subsp. enterica serovar Typhi
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Salmonella enterica subsp. enterica serovar Typhi
Cloned(Commentary) (protein specific)
Commentary
Organism
gene tviB located in the viaB locus, genetic organization, expression as C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
Salmonella enterica subsp. enterica serovar Typhi
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Salmonella enterica subsp. enterica serovar Typhi
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.077
-
UDP-N-acetyl-D-glucosamine
pH 7.5, 25C, recombinant enzyme
Salmonella enterica subsp. enterica serovar Typhi
0.276
-
NAD+
pH 7.5, 25C, recombinant enzyme
Salmonella enterica subsp. enterica serovar Typhi
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Salmonella enterica subsp. enterica serovar Typhi
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
47675
-
3 * 47675, subunit amino acid sequence calculation
Salmonella enterica subsp. enterica serovar Typhi
120500
-
recombinant enzyme, gel filtration
Salmonella enterica subsp. enterica serovar Typhi
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-N-acetyl-D-glucosamine + NAD+ + H2O
Salmonella enterica subsp. enterica serovar Typhi
the enzyme is involved in the biosynthesis of the Vi antigen, a clinically important vaccine antigen, in Salmonella typhi, pathway overview
UDP-N-acetyl-2-amino-2-deoxy-D-glucuronate + NADH
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant C-terminally His6-tagged TviB from Escherichia coli strain BL21(DE3) by two steps of anion exchange chromatography, affinity chromatography, and gel filtration, inactive oligomeric forms of the enzym eis removed in gel filtration, to near homogeneity
Salmonella enterica subsp. enterica serovar Typhi
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Salmonella enterica subsp. enterica serovar Typhi
Storage Stability (protein specific)
Storage Stability
Organism
-80C, purified recombinant enzyme, in presence of 10 mM DTT, stable, even to multiple freeze-thaw cycles
Salmonella enterica subsp. enterica serovar Typhi
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
determination of substrate specificity, no or poor activity with UDP-D-glucose, UDP-D-galactose, and UDP-D-galactosamine, overview
667737
Salmonella enterica subsp. enterica serovar Typhi
?
-
-
-
-
UDP-N-acetyl-D-glucosamine + NAD+ + H2O
-
667737
Salmonella enterica subsp. enterica serovar Typhi
UDP-N-acetyl-2-amino-2-deoxy-D-glucuronate + NADH
product determination by NMR
-
-
?
UDP-N-acetyl-D-glucosamine + NAD+ + H2O
the enzyme is involved in the biosynthesis of the Vi antigen, a clinically important vaccine antigen, in Salmonella typhi, pathway overview
667737
Salmonella enterica subsp. enterica serovar Typhi
UDP-N-acetyl-2-amino-2-deoxy-D-glucuronate + NADH
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
trimer
3 * 47675, subunit amino acid sequence calculation
Salmonella enterica subsp. enterica serovar Typhi
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
30
assay at
Salmonella enterica subsp. enterica serovar Typhi
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
15.5
-
NAD+
pH 7.5, 25C, recombinant enzyme
Salmonella enterica subsp. enterica serovar Typhi
15.5
-
UDP-N-acetyl-D-glucosamine
pH 7.5, 25C, recombinant enzyme
Salmonella enterica subsp. enterica serovar Typhi
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
-
Salmonella enterica subsp. enterica serovar Typhi
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
8.4
inactive above pH 8.4
Salmonella enterica subsp. enterica serovar Typhi
Other publictions for EC 1.1.1.136
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725107
Wang
Structural and genetic charact ...
Escherichia coli
Glycobiology
22
1321-1331
2012
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1
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2
-
7
2
-
-
4
-
-
-
-
-
-
-
-
1
-
1
1
-
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
7
2
-
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-
-
-
-
-
1
-
1
1
-
2
1
-
-
-
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-
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-
712456
Gu
Biosynthesis of a new UDP-suga ...
Bacillus cereus, Bacillus cereus subsp. cytotoxis, NVH 391-98
J. Biol. Chem.
285
24825-24833
2010
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-
1
-
-
-
-
-
-
-
1
-
-
8
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
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-
1
-
-
-
-
-
-
-
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1
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-
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-
4
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
667737
Zhang
Vi antigen biosynthesis in Sal ...
Salmonella enterica subsp. enterica serovar Typhi
Biochemistry
45
8163-8173
2006
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-
1
-
-
-
-
2
-
1
2
1
-
3
-
-
1
-
-
-
1
1
3
1
1
-
-
2
1
1
-
1
-
-
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-
-
1
1
-
-
-
-
-
-
2
-
1
2
1
-
-
-
1
-
-
1
1
3
1
1
-
-
2
1
1
-
-
-
-
-
-
-
-
656241
Miller
Biochemical characterization o ...
Pseudomonas aeruginosa
J. Biol. Chem.
279
37551-37558
2004
2
-
1
-
-
-
-
1
-
1
3
-
-
3
-
-
1
-
-
-
-
1
1
1
1
-
-
-
1
-
-
1
-
2
-
2
-
1
1
-
-
-
-
-
-
1
-
1
3
-
-
-
-
1
-
-
-
1
1
1
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2273
Kawamura
Biosynthesis of UDP-N-acetyl-D ...
Micrococcus luteus
J. Biochem.
98
105-116
1985
1
-
-
-
-
2
3
2
-
-
-
1
-
3
-
-
1
-
-
-
1
1
1
-
-
-
-
-
2
-
-
1
-
-
-
1
-
-
1
-
-
2
-
3
-
2
-
-
-
1
-
-
-
1
-
-
1
1
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
285850
Wheat
Cellular localization of uridi ...
Citrobacter freundii
J. Bacteriol.
127
1032-1035
1976
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
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1
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1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285848
Fan
UDPacetylglucosamine dehydroge ...
Alcaligenes sp.
Arch. Biochem. Biophys.
135
45-49
1969
-
-
-
-
-
-
1
2
-
-
-
1
-
2
-
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3
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1
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2
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2
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1
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2
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1
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3
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1
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