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show all sequences of 1.1.1.136

Biochemical characterization of WbpA, a UDP-N-acetyl-D-glucosamine 6-dehydrogenase involved in O-antigen biosynthesis in Pseudomonas aeruginosa PAO1

Miller, W.L.; Wenzel, C.Q.; Daniels, C.; Larocque, S.; Brisson, J.R.; Lam, J.S.; J. Biol. Chem. 279, 37551-37558 (2004)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
tetramethylammonium, dimethylammonium or sodium slats do not substitute for NH4+ or K+
Pseudomonas aeruginosa
NH4+
or K+, required
Pseudomonas aeruginosa
Cloned(Commentary)
Commentary
Organism
-
Pseudomonas aeruginosa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
positive cooperativity with respect to substrate binding, Hill coefficient 1.8
Pseudomonas aeruginosa
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
K+
or NH4+, required
Pseudomonas aeruginosa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50300
-
3 * 50300, SDS-PAGE
Pseudomonas aeruginosa
153000
-
dynamic light scattering
Pseudomonas aeruginosa
162000
-
gel filtration
Pseudomonas aeruginosa
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas aeruginosa
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant protein with His-tag
Pseudomonas aeruginosa
Storage Stability
Storage Stability
Organism
-20C, 25% glycerol, stable for months
Pseudomonas aeruginosa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-N-acetyl-D-glucosamine + 2 NAD+ + H2O
-
656241
Pseudomonas aeruginosa
UDP-N-acetyl-2-amino-2-deoxy-D-glucuronate + 2 NADH + 2 H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
trimer
3 * 50300, SDS-PAGE
Pseudomonas aeruginosa
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
33
37
-
Pseudomonas aeruginosa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
9
-
Pseudomonas aeruginosa
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas aeruginosa
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Pseudomonas aeruginosa
isoelectric focusing
-
5.5
Pseudomonas aeruginosa
predicted from gene sequence
-
6
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
tetramethylammonium, dimethylammonium or sodium slats do not substitute for NH4+ or K+
Pseudomonas aeruginosa
NH4+
or K+, required
Pseudomonas aeruginosa
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas aeruginosa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas aeruginosa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
positive cooperativity with respect to substrate binding, Hill coefficient 1.8
Pseudomonas aeruginosa
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
K+
or NH4+, required
Pseudomonas aeruginosa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50300
-
3 * 50300, SDS-PAGE
Pseudomonas aeruginosa
153000
-
dynamic light scattering
Pseudomonas aeruginosa
162000
-
gel filtration
Pseudomonas aeruginosa
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant protein with His-tag
Pseudomonas aeruginosa
Storage Stability (protein specific)
Storage Stability
Organism
-20C, 25% glycerol, stable for months
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-N-acetyl-D-glucosamine + 2 NAD+ + H2O
-
656241
Pseudomonas aeruginosa
UDP-N-acetyl-2-amino-2-deoxy-D-glucuronate + 2 NADH + 2 H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
trimer
3 * 50300, SDS-PAGE
Pseudomonas aeruginosa
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
33
37
-
Pseudomonas aeruginosa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
9
-
Pseudomonas aeruginosa
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Pseudomonas aeruginosa
isoelectric focusing
-
5.5
Pseudomonas aeruginosa
predicted from gene sequence
-
6
Other publictions for EC 1.1.1.136
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725107
Wang
Structural and genetic charact ...
Escherichia coli
Glycobiology
22
1321-1331
2012
-
-
1
-
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2
-
7
2
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-
4
-
-
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-
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1
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1
1
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2
1
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-
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1
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2
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7
2
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-
-
-
-
1
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1
1
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2
1
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-
-
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-
-
-
-
-
712456
Gu
Biosynthesis of a new UDP-suga ...
Bacillus cereus, Bacillus cereus subsp. cytotoxis, NVH 391-98
J. Biol. Chem.
285
24825-24833
2010
-
-
1
-
-
-
-
-
-
-
1
-
-
8
-
-
-
-
-
-
-
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4
1
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1
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1
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4
1
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667737
Zhang
Vi antigen biosynthesis in Sal ...
Salmonella enterica subsp. enterica serovar Typhi
Biochemistry
45
8163-8173
2006
-
-
1
-
-
-
-
2
-
1
2
1
-
3
-
-
1
-
-
-
1
1
3
1
1
-
-
2
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
1
2
1
-
-
-
1
-
-
1
1
3
1
1
-
-
2
1
1
-
-
-
-
-
-
-
-
656241
Miller
Biochemical characterization o ...
Pseudomonas aeruginosa
J. Biol. Chem.
279
37551-37558
2004
2
-
1
-
-
-
-
1
-
1
3
-
-
3
-
-
1
-
-
-
-
1
1
1
1
-
-
-
1
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1
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2
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2
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1
1
-
-
-
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-
-
1
-
1
3
-
-
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-
1
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-
1
1
1
1
-
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1
-
-
2
-
-
-
-
-
-
2273
Kawamura
Biosynthesis of UDP-N-acetyl-D ...
Micrococcus luteus
J. Biochem.
98
105-116
1985
1
-
-
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-
2
3
2
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1
-
3
-
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1
-
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1
1
1
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2
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1
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1
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1
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2
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3
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2
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1
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1
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1
1
1
-
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2
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-
285850
Wheat
Cellular localization of uridi ...
Citrobacter freundii
J. Bacteriol.
127
1032-1035
1976
-
-
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1
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1
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285848
Fan
UDPacetylglucosamine dehydroge ...
Alcaligenes sp.
Arch. Biochem. Biophys.
135
45-49
1969
-
-
-
-
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1
2
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1
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2
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3
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1
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2
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2
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1
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2
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1
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3
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1
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