BRENDA - Enzyme Database show
show all sequences of 1.1.1.136

Biosynthesis of UDP-N-acetyl-D-glucosaminuronic acid and UDP-N-acetyl-D-mannosaminuronic acid in Micrococcus luteus

Kawamura, T.; Ichihara, N.; Sugiyama, S.; Yokota, H.; Ishimoto, N.; Ito, E.; J. Biochem. 98, 105-116 (1985)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
dithiothreitol
and the particulate fraction required for full activity
Micrococcus luteus
General Stability
General Stability
Organism
ammonium sulfate protects against inactivation during storage and incubation
Micrococcus luteus
monovalent cations protect against inactivation during incubation
Micrococcus luteus
Inhibitors
Inhibitors
Commentary
Organism
Structure
ethanol
10 mM, more than 90% inhibition
Micrococcus luteus
N-acetylglucosamine 1-phosphate
-
Micrococcus luteus
p-chloromercuribenzoate
0.027 mM, complete inhibition
Micrococcus luteus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.28
-
UDP-N-acetyl-D-glucosamine
-
Micrococcus luteus
1.43
-
NAD+
-
Micrococcus luteus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-N-acetyl-D-glucosamine + 2 NAD+ + H2O
Micrococcus luteus
-
UDP-N-acetyl-2-amino-2-deoxy-D-glucuronate + 2 NADH + 2 H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Micrococcus luteus
-
ATCC 4698
-
Purification (Commentary)
Commentary
Organism
partial
Micrococcus luteus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
41
-
-
Micrococcus luteus
Storage Stability
Storage Stability
Organism
4░C, 1 M (NH4)2SO4, stable for more than 3 weeks
Micrococcus luteus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-N-acetyl-D-glucosamine + 2 NAD+ + H2O
-
2273
Micrococcus luteus
UDP-N-acetyl-2-amino-2-deoxy-D-glucuronate + 2 NADH + 2 H+
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.8
-
above, Tris buffer
Micrococcus luteus
10
-
above, glycine-NaOH buffer
Micrococcus luteus
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Micrococcus luteus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
dithiothreitol
and the particulate fraction required for full activity
Micrococcus luteus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Micrococcus luteus
General Stability (protein specific)
General Stability
Organism
ammonium sulfate protects against inactivation during storage and incubation
Micrococcus luteus
monovalent cations protect against inactivation during incubation
Micrococcus luteus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ethanol
10 mM, more than 90% inhibition
Micrococcus luteus
N-acetylglucosamine 1-phosphate
-
Micrococcus luteus
p-chloromercuribenzoate
0.027 mM, complete inhibition
Micrococcus luteus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.28
-
UDP-N-acetyl-D-glucosamine
-
Micrococcus luteus
1.43
-
NAD+
-
Micrococcus luteus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-N-acetyl-D-glucosamine + 2 NAD+ + H2O
Micrococcus luteus
-
UDP-N-acetyl-2-amino-2-deoxy-D-glucuronate + 2 NADH + 2 H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Micrococcus luteus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
41
-
-
Micrococcus luteus
Storage Stability (protein specific)
Storage Stability
Organism
4░C, 1 M (NH4)2SO4, stable for more than 3 weeks
Micrococcus luteus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-N-acetyl-D-glucosamine + 2 NAD+ + H2O
-
2273
Micrococcus luteus
UDP-N-acetyl-2-amino-2-deoxy-D-glucuronate + 2 NADH + 2 H+
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.8
-
above, Tris buffer
Micrococcus luteus
10
-
above, glycine-NaOH buffer
Micrococcus luteus
Other publictions for EC 1.1.1.136
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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1
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1
1
1
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2
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-
2273
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Biosynthesis of UDP-N-acetyl-D ...
Micrococcus luteus
J. Biochem.
98
105-116
1985
1
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-
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-
2
3
2
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-
-
1
-
3
-
-
1
-
-
-
1
1
1
-
-
-
-
-
2
-
-
1
-
-
-
1
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-
1
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2
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3
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2
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1
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1
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2
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-
-
-
-
-
-
1
-
-
-
-
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-
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1
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-
1
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-
-
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-
285848
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-
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-
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1
2
-
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1
-
2
-
-
-
-
-
-
-
-
3
-
-
-
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-
1
-
-
2
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2
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1
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2
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1
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3
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1
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