BRENDA - Enzyme Database show
show all sequences of 1.1.1.133

Identification of an L-rhamnose synthetic pathway in two nucleocytoplasmic large DNA viruses

Parakkottil Chothi, M.; Duncan, G.; Armirotti, A.; Abergel, C.; Gurnon, J.; Van Etten, J.; Bernardi, C.; Damonte, G.; Tonetti, M.; J. Virol. 84, 8829-8838 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene L780, transcription profiling of UGER, phylogenetic analysis
Acanthamoeba polyphaga Mimivirus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.83
-
UDP-4-keto-6-deoxy-D-glucose
25°C, pH 7.5
Acanthamoeba polyphaga Mimivirus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
divalent metal ions do not alter activity
Acanthamoeba polyphaga Mimivirus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
x * 33000, UGER, SDS-PAGE
Acanthamoeba polyphaga Mimivirus
47000
-
gel filtration
Acanthamoeba polyphaga Mimivirus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acanthamoeba polyphaga Mimivirus
E3VXL5
gene L780
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0011
-
25°C, pH 7.5
Acanthamoeba polyphaga Mimivirus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-4-keto-6-deoxy-D-glucose + NADPH + H+
-
716065
Acanthamoeba polyphaga Mimivirus
UDP-L-rhamnose + NADP+
product identification by electrospray ionization-mass spectrometry and gas chromatography mass spectrometry
-
-
?
Subunits
Subunits
Commentary
Organism
monomer or dimer
x * 33000, UGER, SDS-PAGE
Acanthamoeba polyphaga Mimivirus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
42
-
stable for 30 min
Acanthamoeba polyphaga Mimivirus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8.5
-
Acanthamoeba polyphaga Mimivirus
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
specific
Acanthamoeba polyphaga Mimivirus
Cloned(Commentary) (protein specific)
Commentary
Organism
gene L780, transcription profiling of UGER, phylogenetic analysis
Acanthamoeba polyphaga Mimivirus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
specific
Acanthamoeba polyphaga Mimivirus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.83
-
UDP-4-keto-6-deoxy-D-glucose
25°C, pH 7.5
Acanthamoeba polyphaga Mimivirus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
divalent metal ions do not alter activity
Acanthamoeba polyphaga Mimivirus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
x * 33000, UGER, SDS-PAGE
Acanthamoeba polyphaga Mimivirus
47000
-
gel filtration
Acanthamoeba polyphaga Mimivirus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0011
-
25°C, pH 7.5
Acanthamoeba polyphaga Mimivirus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-4-keto-6-deoxy-D-glucose + NADPH + H+
-
716065
Acanthamoeba polyphaga Mimivirus
UDP-L-rhamnose + NADP+
product identification by electrospray ionization-mass spectrometry and gas chromatography mass spectrometry
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer or dimer
x * 33000, UGER, SDS-PAGE
Acanthamoeba polyphaga Mimivirus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
42
-
stable for 30 min
Acanthamoeba polyphaga Mimivirus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8.5
-
Acanthamoeba polyphaga Mimivirus
Other publictions for EC 1.1.1.133
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724529
Wang
Novel inhibitors of Mycobacter ...
Mycobacterium tuberculosis
Bioorg. Med. Chem. Lett.
21
7064-7067
2011
-
-
-
-
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4
-
-
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3
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4
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4
4
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-
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-
-
-
-
-
-
-
-
-
716065
Parakkottil Chothi
Identification of an L-rhamnos ...
Acanthamoeba polyphaga Mimivirus
J. Virol.
84
8829-8838
2010
-
-
1
-
-
-
-
1
-
1
2
-
-
1
-
-
-
-
-
-
1
-
1
1
-
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1
-
1
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1
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-
1
1
-
-
-
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1
-
1
2
-
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-
-
1
-
1
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
661486
Elling
An enzyme module system for th ...
Salmonella enterica
ChemBioChem
6
1423-1430
2005
-
1
1
-
-
-
-
-
-
-
-
1
-
1
-
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2
-
1
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1
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1
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1
1
1
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1
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2
-
1
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1
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-
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-
-
-
-
668041
Kang
Preparative synthesis of dTDP- ...
Mesorhizobium loti
Biotechnol. Bioeng.
93
21-27
2005
-
1
1
-
-
-
-
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1
-
3
-
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1
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1
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1
1
1
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1
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1
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657064
Watt
A bifunctional 3,5-epimerase/4 ...
Arabidopsis thaliana
Plant Physiol.
134
1337-1346
2004
-
-
1
-
-
-
1
2
-
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2
-
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1
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1
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4
-
1
4
1
1
1
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1
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2
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1
2
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1
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2
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2
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1
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4
-
1
4
1
1
1
-
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-
1
-
-
-
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-
-
657358
Blankenfeldt
Variation on a theme of SDR: d ...
Salmonella enterica
Structure
10
773-786
2002
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1
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1
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2
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285845
Nakano
Thymidine diphosphate-6-deoxy- ...
Aggregatibacter actinomycetemcomitans, Escherichia coli, Escherichia coli O45
J. Biol. Chem.
275
6806-6812
2000
-
-
1
-
-
-
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2
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6
-
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1
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1
2
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2
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1
2
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2
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1
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1
2
-
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-
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-
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-
-
-
-
285844
Giraud
-
Overexpression, purification, ...
Escherichia coli, Escherichia coli K12, Salmonella enterica, Shigella flexneri, Streptomyces mutans, Xanthomonas campestris
Acta Crystallogr. Sect. D
55
2043-2046
1999
-
-
1
1
-
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5
6
-
166
-
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1
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6
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5
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1
5
1
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5
6
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1
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6
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285846
Graninger
Characterization of dTDP-4-deh ...
Salmonella enterica
J. Biol. Chem.
274
25069-25077
1999
-
-
1
-
-
-
2
2
-
-
3
-
-
2
-
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1
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1
1
2
-
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2
2
2
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1
4
-
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1
4
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2
-
2
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3
-
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1
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1
1
2
-
-
-
2
2
2
-
1
-
-
-
-
-
-
-
285843
Melo
The mechanism of 6-deoxyhexose ...
Pseudomonas aeruginosa
J. Biol. Chem.
243
1475-1478
1968
-
-
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1
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-
1
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1
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1
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1
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1
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1
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1
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1
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