BRENDA - Enzyme Database show
show all sequences of 1.1.1.133

Preparative synthesis of dTDP-L-rhamnose through combined enzymatic pathways

Kang, Y.; Yang, Y.; Lee, K.; Lee, S.; Sohng, J.K.; Lee, H.C.; Liou, K.; Kim, B.; Biotechnol. Bioeng. 93, 21-27 (2005)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
six enzymes, including the dTDP-4-keto-rhamnose reductase, are involved in the pathway and are prepared by recombinant expression in Escherichia coli for large scale production of O-antigen precursor sTDP-L-rhamnose in a one-pot reaction, overview
Mesorhizobium loti
Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Mesorhizobium loti
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dTDP-6-deoxy-L-mannose + NADP+
Mesorhizobium loti
-
dTDP-4-dehydro-6-deoxy-L-mannose + NADPH
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mesorhizobium loti
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dTDP-6-deoxy-L-mannose + NADP+
-
668041
Mesorhizobium loti
dTDP-4-dehydro-6-deoxy-L-mannose + NADPH
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Mesorhizobium loti
Application (protein specific)
Application
Commentary
Organism
synthesis
six enzymes, including the dTDP-4-keto-rhamnose reductase, are involved in the pathway and are prepared by recombinant expression in Escherichia coli for large scale production of O-antigen precursor sTDP-L-rhamnose in a one-pot reaction, overview
Mesorhizobium loti
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Mesorhizobium loti
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Mesorhizobium loti
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dTDP-6-deoxy-L-mannose + NADP+
Mesorhizobium loti
-
dTDP-4-dehydro-6-deoxy-L-mannose + NADPH
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dTDP-6-deoxy-L-mannose + NADP+
-
668041
Mesorhizobium loti
dTDP-4-dehydro-6-deoxy-L-mannose + NADPH
-
-
-
?
Other publictions for EC 1.1.1.133
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724529
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668041
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Preparative synthesis of dTDP- ...
Mesorhizobium loti
Biotechnol. Bioeng.
93
21-27
2005
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1
1
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657064
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Plant Physiol.
134
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Structure
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773-786
2002
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285845
Nakano
Thymidine diphosphate-6-deoxy- ...
Aggregatibacter actinomycetemcomitans, Escherichia coli, Escherichia coli O45
J. Biol. Chem.
275
6806-6812
2000
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285844
Giraud
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Overexpression, purification, ...
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Acta Crystallogr. Sect. D
55
2043-2046
1999
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166
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285846
Graninger
Characterization of dTDP-4-deh ...
Salmonella enterica
J. Biol. Chem.
274
25069-25077
1999
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285843
Melo
The mechanism of 6-deoxyhexose ...
Pseudomonas aeruginosa
J. Biol. Chem.
243
1475-1478
1968
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