BRENDA - Enzyme Database show
show all sequences of 1.1.1.133

A bifunctional 3,5-epimerase/4-keto reductase for nucleotide-rhamnose synthesis in Arabidopsis

Watt, G.; Leoff, C.; Harper, A.D.; Bar-Peled, M.; Plant Physiol. 134, 1337-1346 (2004)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Arabidopsis thaliana
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
not inhibitory: MgCl2, CaCl2, EDTA at 10mM
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.009
-
NADPH
pH 8.5, 30C
Arabidopsis thaliana
0.0169
-
dTDP-4-keto-6-deoxy-D-glucose
pH 8.5, 30C
Arabidopsis thaliana
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
x * 35500, deduced from gene sequence, x * 35000, SDS-PAGE
Arabidopsis thaliana
35500
-
x * 35500, deduced from gene sequence, x * 35000, SDS-PAGE
Arabidopsis thaliana
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q9LQ04
bifunctional 3,5-epimerase/4-keto reductase
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme
Arabidopsis thaliana
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
-
Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
additional information
present in all tissues examined
Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-
Storage Stability
Storage Stability
Organism
-20C, repeated freezing and thawing, loss of more than 60% of activity
Arabidopsis thaliana
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dTDP-4-dehydro-6-deoxy-D-glucose + NADH + H+
40% of the rate with NADPH
657064
Arabidopsis thaliana
dTDP-beta-L-rhamnose + NAD+
-
-
-
?
dTDP-4-dehydro-6-deoxy-D-glucose + NADPH
-
657064
Arabidopsis thaliana
dTDP-beta-L-rhamnose + NADP+
-
-
-
?
additional information
no substrate: GDP-4-keto-6-deoxy-mannose, UDP-glucose, dTDP-glucose, UDP-xylose, GDP-fucose
657064
Arabidopsis thaliana
?
-
-
-
-
UDP-4-dehydro-6-deoxy-D-glucose + NADPH
-
657064
Arabidopsis thaliana
UDP-beta-L-rhamnose + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 35500, deduced from gene sequence, x * 35000, SDS-PAGE
Arabidopsis thaliana
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
-
Arabidopsis thaliana
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
45
-
about 35% of maximum activity
Arabidopsis thaliana
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
7.5
-
Arabidopsis thaliana
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Arabidopsis thaliana
NADPH
-
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Arabidopsis thaliana
NADPH
-
Arabidopsis thaliana
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
not inhibitory: MgCl2, CaCl2, EDTA at 10mM
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.009
-
NADPH
pH 8.5, 30C
Arabidopsis thaliana
0.0169
-
dTDP-4-keto-6-deoxy-D-glucose
pH 8.5, 30C
Arabidopsis thaliana
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
x * 35500, deduced from gene sequence, x * 35000, SDS-PAGE
Arabidopsis thaliana
35500
-
x * 35500, deduced from gene sequence, x * 35000, SDS-PAGE
Arabidopsis thaliana
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme
Arabidopsis thaliana
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
-
Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
additional information
present in all tissues examined
Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-
Storage Stability (protein specific)
Storage Stability
Organism
-20C, repeated freezing and thawing, loss of more than 60% of activity
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dTDP-4-dehydro-6-deoxy-D-glucose + NADH + H+
40% of the rate with NADPH
657064
Arabidopsis thaliana
dTDP-beta-L-rhamnose + NAD+
-
-
-
?
dTDP-4-dehydro-6-deoxy-D-glucose + NADPH
-
657064
Arabidopsis thaliana
dTDP-beta-L-rhamnose + NADP+
-
-
-
?
additional information
no substrate: GDP-4-keto-6-deoxy-mannose, UDP-glucose, dTDP-glucose, UDP-xylose, GDP-fucose
657064
Arabidopsis thaliana
?
-
-
-
-
UDP-4-dehydro-6-deoxy-D-glucose + NADPH
-
657064
Arabidopsis thaliana
UDP-beta-L-rhamnose + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 35500, deduced from gene sequence, x * 35000, SDS-PAGE
Arabidopsis thaliana
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
-
Arabidopsis thaliana
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
45
-
about 35% of maximum activity
Arabidopsis thaliana
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
7.5
-
Arabidopsis thaliana
Other publictions for EC 1.1.1.133
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724529
Wang
Novel inhibitors of Mycobacter ...
Mycobacterium tuberculosis
Bioorg. Med. Chem. Lett.
21
7064-7067
2011
-
-
-
-
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4
-
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3
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4
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4
4
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-
716065
Parakkottil Chothi
Identification of an L-rhamnos ...
Acanthamoeba polyphaga Mimivirus
J. Virol.
84
8829-8838
2010
-
-
1
-
-
-
-
1
-
1
2
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1
-
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-
-
-
-
1
-
1
1
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1
-
1
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1
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1
1
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-
-
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-
1
-
1
2
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-
-
1
-
1
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
661486
Elling
An enzyme module system for th ...
Salmonella enterica
ChemBioChem
6
1423-1430
2005
-
1
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
1
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1
-
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1
-
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1
1
1
-
-
-
-
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-
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1
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-
-
2
-
1
-
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-
1
-
-
-
-
-
-
-
-
-
668041
Kang
Preparative synthesis of dTDP- ...
Mesorhizobium loti
Biotechnol. Bioeng.
93
21-27
2005
-
1
1
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
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1
1
1
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-
-
-
-
-
-
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-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657064
Watt
A bifunctional 3,5-epimerase/4 ...
Arabidopsis thaliana
Plant Physiol.
134
1337-1346
2004
-
-
1
-
-
-
1
2
-
-
2
-
-
1
-
-
1
-
-
4
-
1
4
1
1
1
-
-
-
1
-
2
-
-
-
-
-
1
2
-
-
-
-
1
-
2
-
-
2
-
-
-
-
1
-
4
-
1
4
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
657358
Blankenfeldt
Variation on a theme of SDR: d ...
Salmonella enterica
Structure
10
773-786
2002
-
-
-
1
-
-
-
-
-
1
-
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2
-
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1
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1
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285845
Nakano
Thymidine diphosphate-6-deoxy- ...
Aggregatibacter actinomycetemcomitans, Escherichia coli, Escherichia coli O45
J. Biol. Chem.
275
6806-6812
2000
-
-
1
-
-
-
-
-
-
-
2
-
-
6
-
-
1
-
-
-
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1
2
-
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-
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-
2
-
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1
2
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2
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1
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1
2
-
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-
-
-
-
-
-
-
-
-
285844
Giraud
-
Overexpression, purification, ...
Escherichia coli, Escherichia coli K12, Salmonella enterica, Shigella flexneri, Streptomyces mutans, Xanthomonas campestris
Acta Crystallogr. Sect. D
55
2043-2046
1999
-
-
1
1
-
-
-
-
-
-
5
6
-
166
-
-
1
-
-
-
-
-
6
-
-
-
-
-
-
-
-
5
-
-
-
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1
5
1
-
-
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5
6
-
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1
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-
-
6
-
-
-
-
-
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-
-
-
-
-
-
-
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-
285846
Graninger
Characterization of dTDP-4-deh ...
Salmonella enterica
J. Biol. Chem.
274
25069-25077
1999
-
-
1
-
-
-
2
2
-
-
3
-
-
2
-
-
1
-
-
-
1
1
2
-
-
-
2
2
2
-
1
4
-
-
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-
1
4
-
-
-
-
2
-
2
-
-
3
-
-
-
-
1
-
-
1
1
2
-
-
-
2
2
2
-
1
-
-
-
-
-
-
-
285843
Melo
The mechanism of 6-deoxyhexose ...
Pseudomonas aeruginosa
J. Biol. Chem.
243
1475-1478
1968
-
-
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1
-
-
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1
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1
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1
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1
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1
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1
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1
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1
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