BRENDA - Enzyme Database show
show all sequences of 1.1.1.133

Overexpression, purification, crystallization and preliminary structural study of dTDP-6-deoxy-L-lyxo-4-hexulose reductase (RmlD), the fourth enzyme of the dTDP-L-rhamnose synthesis pathway, from Salmonella enterica serovar typhimurium

Giraud, M.F.; McMiken, H.J.; Leonard, G.A.; Messner, P.; Whitfield, C.; Naismith, J.H.; Acta Crystallogr. Sect. D 55, 2043-2046 (1999)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
native overexpressed in Escherichia coli BL21(DE3) and selenomethionine-enriched in B834(lambdaDE3)
Salmonella enterica
Crystallization (Commentary)
Crystallization
Organism
sitting drop vapour-diffusion method, 6 d
Salmonella enterica
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32500
-
HPLC
Salmonella enterica
32540
-
mass spectrometry, native protein
Salmonella enterica
32560
-
deduced from sequence
Salmonella enterica
32730
-
mass spectrometry, selenomethionine-enriched protein
Salmonella enterica
49500
-
light-scattering
Salmonella enterica
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH
Escherichia coli
synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria
dTDP-L-rhamnose + NADP+
-
Escherichia coli
?
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH
Xanthomonas campestris
synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria
dTDP-L-rhamnose + NADP+
-
Xanthomonas campestris
?
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH
Salmonella enterica
synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria
dTDP-L-rhamnose + NADP+
-
Salmonella enterica
?
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH
Shigella flexneri
synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria
dTDP-L-rhamnose + NADP+
-
Shigella flexneri
?
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH
Streptomyces mutans
synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria
dTDP-L-rhamnose + NADP+
-
Streptomyces mutans
?
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH
Escherichia coli K12
synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria
dTDP-L-rhamnose + NADP+
-
Escherichia coli K12
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
K12
-
Escherichia coli K12
-
K12
-
Salmonella enterica
-
-
-
Shigella flexneri
-
-
-
Streptomyces mutans
-
-
-
Xanthomonas campestris
-
-
-
Purification (Commentary)
Commentary
Organism
anion-exchange, hydrophobic chromatography
Salmonella enterica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH
synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria
285844
Escherichia coli
dTDP-L-rhamnose + NADP+
-
285844
Escherichia coli
?
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH
synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria
285844
Xanthomonas campestris
dTDP-L-rhamnose + NADP+
-
285844
Xanthomonas campestris
?
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH
synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria
285844
Salmonella enterica
dTDP-L-rhamnose + NADP+
-
285844
Salmonella enterica
?
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH
synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria
285844
Shigella flexneri
dTDP-L-rhamnose + NADP+
-
285844
Shigella flexneri
?
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH
synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria
285844
Streptomyces mutans
dTDP-L-rhamnose + NADP+
-
285844
Streptomyces mutans
?
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH
synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria
285844
Escherichia coli K12
dTDP-L-rhamnose + NADP+
-
285844
Escherichia coli K12
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Shigella flexneri
NADPH
-
Streptomyces mutans
NADPH
-
Xanthomonas campestris
NADPH
-
Escherichia coli
NADPH
-
Salmonella enterica
Cloned(Commentary) (protein specific)
Commentary
Organism
native overexpressed in Escherichia coli BL21(DE3) and selenomethionine-enriched in B834(lambdaDE3)
Salmonella enterica
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Shigella flexneri
NADPH
-
Streptomyces mutans
NADPH
-
Xanthomonas campestris
NADPH
-
Escherichia coli
NADPH
-
Salmonella enterica
Crystallization (Commentary) (protein specific)
Crystallization
Organism
sitting drop vapour-diffusion method, 6 d
Salmonella enterica
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32500
-
HPLC
Salmonella enterica
32540
-
mass spectrometry, native protein
Salmonella enterica
32560
-
deduced from sequence
Salmonella enterica
32730
-
mass spectrometry, selenomethionine-enriched protein
Salmonella enterica
49500
-
light-scattering
Salmonella enterica
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH
Escherichia coli
synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria
dTDP-L-rhamnose + NADP+
-
Escherichia coli
?
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH
Xanthomonas campestris
synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria
dTDP-L-rhamnose + NADP+
-
Xanthomonas campestris
?
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH
Salmonella enterica
synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria
dTDP-L-rhamnose + NADP+
-
Salmonella enterica
?
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH
Shigella flexneri
synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria
dTDP-L-rhamnose + NADP+
-
Shigella flexneri
?
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH
Streptomyces mutans
synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria
dTDP-L-rhamnose + NADP+
-
Streptomyces mutans
?
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH
Escherichia coli K12
synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria
dTDP-L-rhamnose + NADP+
-
Escherichia coli K12
?
Purification (Commentary) (protein specific)
Commentary
Organism
anion-exchange, hydrophobic chromatography
Salmonella enterica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH
synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria
285844
Escherichia coli
dTDP-L-rhamnose + NADP+
-
285844
Escherichia coli
?
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH
synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria
285844
Xanthomonas campestris
dTDP-L-rhamnose + NADP+
-
285844
Xanthomonas campestris
?
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH
synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria
285844
Salmonella enterica
dTDP-L-rhamnose + NADP+
-
285844
Salmonella enterica
?
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH
synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria
285844
Shigella flexneri
dTDP-L-rhamnose + NADP+
-
285844
Shigella flexneri
?
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH
synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria
285844
Streptomyces mutans
dTDP-L-rhamnose + NADP+
-
285844
Streptomyces mutans
?
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH
synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria
285844
Escherichia coli K12
dTDP-L-rhamnose + NADP+
-
285844
Escherichia coli K12
?
Other publictions for EC 1.1.1.133
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724529
Wang
Novel inhibitors of Mycobacter ...
Mycobacterium tuberculosis
Bioorg. Med. Chem. Lett.
21
7064-7067
2011
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716065
Parakkottil Chothi
Identification of an L-rhamnos ...
Acanthamoeba polyphaga Mimivirus
J. Virol.
84
8829-8838
2010
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661486
Elling
An enzyme module system for th ...
Salmonella enterica
ChemBioChem
6
1423-1430
2005
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668041
Kang
Preparative synthesis of dTDP- ...
Mesorhizobium loti
Biotechnol. Bioeng.
93
21-27
2005
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1
1
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657064
Watt
A bifunctional 3,5-epimerase/4 ...
Arabidopsis thaliana
Plant Physiol.
134
1337-1346
2004
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1
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1
2
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2
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1
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1
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4
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4
1
1
1
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1
2
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1
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4
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4
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1
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657358
Blankenfeldt
Variation on a theme of SDR: d ...
Salmonella enterica
Structure
10
773-786
2002
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1
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285845
Nakano
Thymidine diphosphate-6-deoxy- ...
Aggregatibacter actinomycetemcomitans, Escherichia coli, Escherichia coli O45
J. Biol. Chem.
275
6806-6812
2000
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1
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285844
Giraud
-
Overexpression, purification, ...
Escherichia coli, Escherichia coli K12, Salmonella enterica, Shigella flexneri, Streptomyces mutans, Xanthomonas campestris
Acta Crystallogr. Sect. D
55
2043-2046
1999
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1
1
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5
6
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166
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285846
Graninger
Characterization of dTDP-4-deh ...
Salmonella enterica
J. Biol. Chem.
274
25069-25077
1999
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285843
Melo
The mechanism of 6-deoxyhexose ...
Pseudomonas aeruginosa
J. Biol. Chem.
243
1475-1478
1968
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