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Literature summary for 1.1.1.132 extracted from
- Comparative characterization of two GDP-mannose dehydrogenase genes from Saccharina japonica (Laminariales, Phaeophyceae) (2016), BMC Plant Biol., 16, 62.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Saccharina japonica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | inhibitor of isoform GMD2 with 71.86% residual activity at 1 mM | Saccharina japonica |  |
| Mn2+ | - |
Saccharina japonica | |
| Zn2+ | potent inhibitor with 0.75% residual activity at 1 mM for both isoforms GMD1 and GMD2 | Saccharina japonica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.139 | - |
NAD+ | isoform GMD1, at pH 8.0 and 30°C | Saccharina japonica | |
| 0.177 | - |
GDP-D-mannose | isoform GMD2, at pH 8.3 and 20°C | Saccharina japonica | |
| 0.195 | - |
NAD+ | isoform GMD2, at pH 8.3 and 20°C | Saccharina japonica | |
| 0.289 | - |
GDP-D-mannose | isoform GMD1, at pH 8.0 and 30°C | Saccharina japonica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activator of isoform GMD1 with 178.05% activity at 1 mM | Saccharina japonica | |
| Mg2+ | 133.79% activity of isoform GMD1 at 1 mM, 120.25% activity of isoform GMD2 at 1 mM | Saccharina japonica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34460 | - |
- |
Saccharina japonica |
| 35200 | - |
- |
Saccharina japonica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-D-mannose + NAD+ + H2O | Saccharina japonica | - |
GDP-D-mannuronate + NADH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharina japonica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| amylose resin column chromatography | Saccharina japonica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-D-mannose + NAD+ + H2O | - |
Saccharina japonica | GDP-D-mannuronate + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34460, isoform GMD2, calculated from amino acid sequence | Saccharina japonica |
| ? | x * 35200, isoform GMD2, calculated from amino acid sequence | Saccharina japonica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GDP-mannose dehydrogenase | - |
Saccharina japonica |
| GMD1 | isoform | Saccharina japonica |
| GMD2 | isoform | Saccharina japonica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
isoform GMD1 | Saccharina japonica |
| 30 | - |
isoform GMD1 | Saccharina japonica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
isoform GMD1 | Saccharina japonica |
| 8.3 | - |
isoform GMD2 | Saccharina japonica |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Saccharina japonica | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Saccharina japonica | isoform GMD1, calculated from amino acid sequence | - |
5.2 |
| Saccharina japonica | isoform GMD2, calculated from amino acid sequence | - |
5.4 |
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Release 2023.1 (January 2023)
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