Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
mucoid strains of cystic fibrosis patients 8821 and its high-alginate-producing spontaneously variant 8821M, enzyme overproduced using 2 different plasmid vectors containing algD under control of the tac promoter mobilized into both strains | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
increase of activity has only limited effect on the stimulation of alginate synthesis, existance of other rate-limiting enzymatic step suggested | Pseudomonas aeruginosa |
0.0058 | - |
+/-0.002, 8821/pMMB24, not IPTG induced | Pseudomonas aeruginosa |
0.0128 | - |
+/-0.0018, 8821/pVD211, not IPTG induced | Pseudomonas aeruginosa |
0.0226 | - |
+/-0.0025, 8821/pVD211, induced by 0.5 mM IPTG | Pseudomonas aeruginosa |
0.0265 | - |
+/-0.003, 8821/pVD211, induced by 3 mM IPTG | Pseudomonas aeruginosa |
0.028 | - |
+/-0.0029, 8821M/pMMB24, not IPTG induced | Pseudomonas aeruginosa |
0.0579 | - |
+/-0.0029, 8821/pVD211, not IPTG induced | Pseudomonas aeruginosa |
0.084 | - |
+/-0.0019, 8821/pVD211, induced by 0.5 mM IPTG | Pseudomonas aeruginosa |
0.0942 | - |
+/-0.004, 8821/pVD211, induced by 3 mM IPTG | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-mannose + NAD+ + H2O | - |
Pseudomonas aeruginosa | GDP-mannuronate + NADH | - |
ir |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas aeruginosa |