Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | 26% inhibition at 1 mM | Pseudomonas aeruginosa | |
D-maltose | 17% inhibition at 1 mM | Pseudomonas aeruginosa | |
GDP-D-glucose | 25% inhibition at 1 mM | Pseudomonas aeruginosa | |
GMP | competitive, 52% inhibition at 1 mM; competitive, most potent | Pseudomonas aeruginosa | |
iodacetamide | 40% inactivation within 30 min, complete reactivation by dithiothreitol | Pseudomonas aeruginosa | |
iodoacetamide | 40% inactivation after 30 min preincubation | Pseudomonas aeruginosa | |
maltose | - |
Pseudomonas aeruginosa | |
additional information | full reactivation by dithiothreitol (25 mM) after p-hydroxymercuribenzoate or iodoacetamide inactivation | Pseudomonas aeruginosa | |
p-hydroxymercuribenzoate | 96% inactivation after 5 min preincubation; 96% inactivation within 5 min, complete reactivation by dithiothreitol | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0149 | - |
GDP-D-mannose | - |
Pseudomonas aeruginosa | |
0.0149 | - |
GDP-D-mannose | 24°C, pH 8.0 | Pseudomonas aeruginosa | |
0.185 | - |
NAD+ | cosubstrate GDP-D-mannose | Pseudomonas aeruginosa | |
0.185 | - |
NAD+ | 24°C, pH 8.0 | Pseudomonas aeruginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Pseudomonas aeruginosa | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
6 * 48000, SDS-PAGE | Pseudomonas aeruginosa |
290000 | - |
gel filtration | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas aeruginosa | key enzyme in alginate biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Pseudomonas aeruginosa | - |
mucoid strain 8821 | - |
Pseudomonas aeruginosa | - |
enzyme overproduced using a plasmid vector containing algD under control of the tac promoter | - |
Purification (Comment) | Organism |
---|---|
pH/heat treatment acetone precipitation HPLC gel filtration chromatography | Pseudomonas aeruginosa |
Renatured (Comment) | Organism |
---|---|
inactivation by iodacetamide or p-hydroxymercuribenzoate is completely reversible | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.068 | - |
- |
Pseudomonas aeruginosa |
1068 | - |
24°C, pH 8.0 | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-D-mannose + NAD+ + H2O | - |
Pseudomonas aeruginosa | GDP-D-mannuronate + NADH | - |
? | |
GDP-mannose + NAD+ + H2O | - |
Pseudomonas aeruginosa | GDP-mannuronate + NADH | - |
? | |
additional information | key enzyme in alginate biosynthesis | Pseudomonas aeruginosa | ? | - |
? | |
additional information | no substrate: D-mannose, UDP-D-mannose, UDP-D-glucose, TDP-D-glucose, CDP-D-glucose | Pseudomonas aeruginosa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 48000, SDS-PAGE | Pseudomonas aeruginosa |
More | N-terminal amino acid sequence | Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
above, 2-3 min, loss of activity | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
- |
Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas aeruginosa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0227 | - |
GMP | 24°C, pH 8.0 | Pseudomonas aeruginosa |