BRENDA - Enzyme Database show
show all sequences of 1.1.1.132

Purification and characterization of guanosine diphospho-D-mannose dehydrogenase. A key enzyme in the biosynthesis of alginate by Pseudomonas aeruginosa

Roychoudhury, S.; Mmay, T.B.; Gill, J.F.; Singh, S.K.; Feingold, D.S.; Chakrabarty, A.M.; J. Biol. Chem. 264, 9380-9385 (1989)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
ATP
; 26% inhibition at 1 mM
Pseudomonas aeruginosa
D-maltose
17% inhibition at 1 mM
Pseudomonas aeruginosa
GDP-D-glucose
; 25% inhibition at 1 mM
Pseudomonas aeruginosa
GMP
competitive, 52% inhibition at 1 mM; competitive, most potent
Pseudomonas aeruginosa
iodacetamide
40% inactivation within 30 min, complete reactivation by dithiothreitol
Pseudomonas aeruginosa
iodoacetamide
40% inactivation after 30 min preincubation
Pseudomonas aeruginosa
maltose
-
Pseudomonas aeruginosa
additional information
full reactivation by dithiothreitol (25 mM) after p-hydroxymercuribenzoate or iodoacetamide inactivation
Pseudomonas aeruginosa
p-hydroxymercuribenzoate
96% inactivation after 5 min preincubation; 96% inactivation within 5 min, complete reactivation by dithiothreitol
Pseudomonas aeruginosa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0149
-
GDP-D-mannose
; 24C, pH 8.0
Pseudomonas aeruginosa
0.185
-
NAD+
24C, pH 8.0; cosubstrate GDP-D-mannose
Pseudomonas aeruginosa
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Pseudomonas aeruginosa
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
48000
-
6 * 48000, SDS-PAGE
Pseudomonas aeruginosa
290000
-
gel filtration
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Pseudomonas aeruginosa
key enzyme in alginate biosynthesis
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas aeruginosa
-
; enzyme overproduced using a plasmid vector containing algD under control of the tac promoter; mucoid strain 8821
-
Purification (Commentary)
Commentary
Organism
pH/heat treatment acetone precipitation HPLC gel filtration chromatography
Pseudomonas aeruginosa
Renatured (Commentary)
Commentary
Organism
inactivation by iodacetamide or p-hydroxymercuribenzoate is completely reversible
Pseudomonas aeruginosa
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.068
-
-
Pseudomonas aeruginosa
1068
-
24C, pH 8.0
Pseudomonas aeruginosa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
GDP-D-mannose + NAD+ + H2O
-
285830
Pseudomonas aeruginosa
GDP-D-mannuronate + NADH
-
-
-
?
GDP-mannose + NAD+ + H2O
-
285830
Pseudomonas aeruginosa
GDP-mannuronate + NADH
-
285830
Pseudomonas aeruginosa
?
additional information
key enzyme in alginate biosynthesis
285830
Pseudomonas aeruginosa
?
-
-
-
-
additional information
no substrate: D-mannose, UDP-D-mannose, UDP-D-glucose, TDP-D-glucose, CDP-D-glucose
285830
Pseudomonas aeruginosa
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
hexamer
6 * 48000, SDS-PAGE
Pseudomonas aeruginosa
More
N-terminal amino acid sequence
Pseudomonas aeruginosa
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
50
-
-
Pseudomonas aeruginosa
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
50
-
above, 2-3 min, loss of activity
Pseudomonas aeruginosa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.7
-
-
Pseudomonas aeruginosa
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas aeruginosa
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0227
-
GMP
24C, pH 8.0
Pseudomonas aeruginosa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas aeruginosa
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ATP
; 26% inhibition at 1 mM
Pseudomonas aeruginosa
D-maltose
17% inhibition at 1 mM
Pseudomonas aeruginosa
GDP-D-glucose
; 25% inhibition at 1 mM
Pseudomonas aeruginosa
GMP
competitive, 52% inhibition at 1 mM; competitive, most potent
Pseudomonas aeruginosa
iodacetamide
40% inactivation within 30 min, complete reactivation by dithiothreitol
Pseudomonas aeruginosa
iodoacetamide
40% inactivation after 30 min preincubation
Pseudomonas aeruginosa
maltose
-
Pseudomonas aeruginosa
additional information
full reactivation by dithiothreitol (25 mM) after p-hydroxymercuribenzoate or iodoacetamide inactivation
Pseudomonas aeruginosa
p-hydroxymercuribenzoate
96% inactivation after 5 min preincubation; 96% inactivation within 5 min, complete reactivation by dithiothreitol
Pseudomonas aeruginosa
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0227
-
GMP
24C, pH 8.0
Pseudomonas aeruginosa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0149
-
GDP-D-mannose
; 24C, pH 8.0
Pseudomonas aeruginosa
0.185
-
NAD+
24C, pH 8.0; cosubstrate GDP-D-mannose
Pseudomonas aeruginosa
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Pseudomonas aeruginosa
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
48000
-
6 * 48000, SDS-PAGE
Pseudomonas aeruginosa
290000
-
gel filtration
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Pseudomonas aeruginosa
key enzyme in alginate biosynthesis
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
pH/heat treatment acetone precipitation HPLC gel filtration chromatography
Pseudomonas aeruginosa
Renatured (Commentary) (protein specific)
Commentary
Organism
inactivation by iodacetamide or p-hydroxymercuribenzoate is completely reversible
Pseudomonas aeruginosa
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.068
-
-
Pseudomonas aeruginosa
1068
-
24C, pH 8.0
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
GDP-D-mannose + NAD+ + H2O
-
285830
Pseudomonas aeruginosa
GDP-D-mannuronate + NADH
-
-
-
?
GDP-mannose + NAD+ + H2O
-
285830
Pseudomonas aeruginosa
GDP-mannuronate + NADH
-
285830
Pseudomonas aeruginosa
?
additional information
key enzyme in alginate biosynthesis
285830
Pseudomonas aeruginosa
?
-
-
-
-
additional information
no substrate: D-mannose, UDP-D-mannose, UDP-D-glucose, TDP-D-glucose, CDP-D-glucose
285830
Pseudomonas aeruginosa
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
hexamer
6 * 48000, SDS-PAGE
Pseudomonas aeruginosa
More
N-terminal amino acid sequence
Pseudomonas aeruginosa
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
50
-
-
Pseudomonas aeruginosa
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
50
-
above, 2-3 min, loss of activity
Pseudomonas aeruginosa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.7
-
-
Pseudomonas aeruginosa
Other publictions for EC 1.1.1.132
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725424
Tenhaken
Characterization of GDP-mannos ...
Ectocarpus siliculosus
J. Biol. Chem.
286
16707-16715
2011
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1
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1
2
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7
3
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1
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1
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1
1
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2
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1
1
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1
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1
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2
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7
3
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1
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1
1
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2
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1
1
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725757
Manzo
Post-transcriptional regulatio ...
Azotobacter vinelandii
J. Mol. Microbiol. Biotechnol.
21
147-159
2011
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1
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1
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1
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-
691949
Li
Effects of ambroxol on alginat ...
Pseudomonas aeruginosa
Curr. Microbiol.
57
1-7
2008
-
-
-
-
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1
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1
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4
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1
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1
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1
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1
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-
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-
701412
Shi
Detection of the key enzyme of ...
Vibrio sp. QY102
World J. Microbiol. Biotechnol.
24
1613-1615
2008
-
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1
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1
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667316
Kimmel
Inactivation of GDP-mannose de ...
Pseudomonas aeruginosa
Arch. Biochem. Biophys.
441
132-140
2005
-
-
1
-
2
-
1
6
-
-
-
1
-
1
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1
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1
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2
1
1
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1
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1
1
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1
1
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2
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1
1
6
-
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1
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1
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1
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
654702
Snook
Crystal structure of GDP-manno ...
Pseudomonas aeruginosa
Biochemistry
42
4658-4668
2003
-
-
-
1
-
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-
-
-
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1
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1
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1
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1
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-
654666
Naught
Allosterism and cooperativity ...
Pseudomonas aeruginosa
Biochemistry
41
9637-9645
2002
-
-
-
-
-
-
-
2
-
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1
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1
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1
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2
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285841
Mitsuya
Influence of macrolides on gua ...
Pseudomonas aeruginosa
J. Infect. Chemother.
6
45-50
2000
-
-
-
-
-
-
2
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-
7
-
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1
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1
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1
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2
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1
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285836
Tavares
Pattern of changes in the acti ...
Pseudomonas aeruginosa
Res. Microbiol.
150
105-116
1999
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1
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1
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1
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285838
Tatnell
Overexpression of the GDP-mann ...
Pseudomonas aeruginosa
Biochem. Soc. Trans.
24
405S
1996
-
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-
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1
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16
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1
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16
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1
1
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285842
Tatnell
GDP-mannose dehydrogenase is t ...
Pseudomonas aeruginosa
Microbiology
140
1745-1754
1994
-
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-
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1
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1
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41
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1
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1
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1
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41
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285839
Elloumi
-
Inhibitors of GDP-mannose dehy ...
Pseudomonas aeruginosa
Eur. J. Med. Chem.
27
149-154
1992
-
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11
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11
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285840
Roychoudhury
Characterization of guanosine ...
Pseudomonas aeruginosa
J. Biol. Chem.
267
990-996
1992
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1
1
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1
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4
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285837
Martins
Alginate biosynthesis in mucoi ...
Pseudomonas aeruginosa
Enzyme Microb. Technol.
13
385-389
1991
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9
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285830
Roychoudhury
Purification and characterizat ...
Pseudomonas aeruginosa
J. Biol. Chem.
264
9380-9385
1989
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9
2
1
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1
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4
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1
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4
2
1
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1
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1
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1
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9
1
2
1
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2
1
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1
1
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2
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4
2
1
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1
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1
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285833
Deretic
Gene algD coding for GDPmannos ...
Pseudomonas aeruginosa
J. Bacteriol.
169
351-358
1987
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2
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285832
Pugashetti
GDPmannose dehydrogenase and b ...
Pseudomonas aeruginosa
J. Bacteriol.
153
1107-1110
1983
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285834
Horan
-
Studies on some enzymes of alg ...
Azotobacter vinelandii
J. Gen. Microbiol.
129
2985-2990
1983
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1
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2
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1
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1
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1
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2
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1
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285835
Couperwhite
Polysaccharide production and ...
Azotobacter vinelandii
Antonie van Leeuwenhoek
41
25-32
1975
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1
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1
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1
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1
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285829
Preiss
-
GDP-mannose dehydrogenase from ...
Arthrobacter sp., Arthrobacter sp. NRRL B1973
Methods Enzymol.
8
285-287
1966
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3
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4
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1
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1
1
4
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1
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1
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1
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3
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1
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1
1
4
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1
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285831
Preiss
Sugar nucleotide reactions in ...
Arthrobacter sp.
J. Biol. Chem.
239
3127-3132
1964
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3
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1
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1
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1
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