BRENDA - Enzyme Database show
show all sequences of 1.1.1.130

beta-Keto-L-gulonic acid as an intermediate in the bacterial metabolism of ascorbic acid

Volk, W.A.; Larsen, J.L.; J. Biol. Chem. 237, 2454-2457 (1962)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
p-chloromercuribenzoate
32% inhibition at 1 mM, not reversal by GSH
Klebsiella aerogenes
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.016
-
NADH
-
Klebsiella aerogenes
0.022
-
NADPH
-
Klebsiella aerogenes
1.16
-
(4R,5S)-4,5,6-trihydroxy-2,3-dioxohexanoate
-
Klebsiella aerogenes
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(4R,5S)-4,5,6-trihydroxy-2,3-dioxohexanoate + NAD(P)H
Klebsiella aerogenes
-
3-dehydro-L-gulonate + NAD(P)+
-
Klebsiella aerogenes
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Klebsiella aerogenes
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Klebsiella aerogenes
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Klebsiella aerogenes
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
8.06
-
-
Klebsiella aerogenes
Storage Stability
Storage Stability
Organism
0°C, 0.03 M Tris-HCl, pH 7.7, 0.62 mg protein/ml, 85% retaining activity in 4 weeks
Klebsiella aerogenes
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(4R,5S)-4,5,6-trihydroxy-2,3-dioxohexanoate + NAD(P)H
-
285827
Klebsiella aerogenes
3-dehydro-L-gulonate + NAD(P)+
-
285827
Klebsiella aerogenes
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Klebsiella aerogenes
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.2
8.4
50% of maximal activity at pH 5.2 and pH 8.4
Klebsiella aerogenes
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
twice effective compared to NADPH
Klebsiella aerogenes
NADPH
NADH 2fold more effective
Klebsiella aerogenes
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
twice effective compared to NADPH
Klebsiella aerogenes
NADPH
NADH 2fold more effective
Klebsiella aerogenes
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
p-chloromercuribenzoate
32% inhibition at 1 mM, not reversal by GSH
Klebsiella aerogenes
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.016
-
NADH
-
Klebsiella aerogenes
0.022
-
NADPH
-
Klebsiella aerogenes
1.16
-
(4R,5S)-4,5,6-trihydroxy-2,3-dioxohexanoate
-
Klebsiella aerogenes
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(4R,5S)-4,5,6-trihydroxy-2,3-dioxohexanoate + NAD(P)H
Klebsiella aerogenes
-
3-dehydro-L-gulonate + NAD(P)+
-
Klebsiella aerogenes
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Klebsiella aerogenes
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Klebsiella aerogenes
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
8.06
-
-
Klebsiella aerogenes
Storage Stability (protein specific)
Storage Stability
Organism
0°C, 0.03 M Tris-HCl, pH 7.7, 0.62 mg protein/ml, 85% retaining activity in 4 weeks
Klebsiella aerogenes
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(4R,5S)-4,5,6-trihydroxy-2,3-dioxohexanoate + NAD(P)H
-
285827
Klebsiella aerogenes
3-dehydro-L-gulonate + NAD(P)+
-
285827
Klebsiella aerogenes
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Klebsiella aerogenes
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.2
8.4
50% of maximal activity at pH 5.2 and pH 8.4
Klebsiella aerogenes
Other publictions for EC 1.1.1.130
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
285827
Volk
beta-Keto-L-gulonic acid as an ...
Klebsiella aerogenes
J. Biol. Chem.
237
2454-2457
1962
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