BRENDA - Enzyme Database show
show all sequences of 1.1.1.129

L-threonic acid dehydrogenase: Purification and properties

Aspen, A.J.; Jakoby, W.B.; J. Biol. Chem. 239, 710-713 (1964)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
hydrazine
inhibitory at 100 mM
Pseudomonas sp.
hydroxylamine
inhibitory at 100 mM
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-threonate + NAD+
Pseudomonas sp.
-
3-dehydro-L-threonate + NADH
-
Pseudomonas sp.
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
-
-
Purification (Commentary)
Commentary
Organism
100fold purification, calcium phosphate gel
Pseudomonas sp.
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
inducible by growing in L-threonate-containing medium
Pseudomonas sp.
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
124
-
-
Pseudomonas sp.
Storage Stability
Storage Stability
Organism
-15°C, 30 mM Tris-HCl, pH 7.5, 5 mM 2-mercaptoethanol, 0.5 M NaCl, 44% retaining activity after 8 weeks
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-threonate + NAD+
-
285825
Pseudomonas sp.
3-dehydro-L-threonate + NADH
-
285825
Pseudomonas sp.
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10.8
-
oxidation reaction
Pseudomonas sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
8.9
11.5
50% of maximal activity at pH 8.9 and pH 11.5
Pseudomonas sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
cannot be replaced by NADP+
Pseudomonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
cannot be replaced by NADP+
Pseudomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
hydrazine
inhibitory at 100 mM
Pseudomonas sp.
hydroxylamine
inhibitory at 100 mM
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-threonate + NAD+
Pseudomonas sp.
-
3-dehydro-L-threonate + NADH
-
Pseudomonas sp.
?
Purification (Commentary) (protein specific)
Commentary
Organism
100fold purification, calcium phosphate gel
Pseudomonas sp.
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
inducible by growing in L-threonate-containing medium
Pseudomonas sp.
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
124
-
-
Pseudomonas sp.
Storage Stability (protein specific)
Storage Stability
Organism
-15°C, 30 mM Tris-HCl, pH 7.5, 5 mM 2-mercaptoethanol, 0.5 M NaCl, 44% retaining activity after 8 weeks
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-threonate + NAD+
-
285825
Pseudomonas sp.
3-dehydro-L-threonate + NADH
-
285825
Pseudomonas sp.
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10.8
-
oxidation reaction
Pseudomonas sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
8.9
11.5
50% of maximal activity at pH 8.9 and pH 11.5
Pseudomonas sp.
Other publictions for EC 1.1.1.129
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
285825
Aspen
L-threonic acid dehydrogenase: ...
Pseudomonas sp.
J. Biol. Chem.
239
710-713
1964
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